Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis
Plant beneficial microbes mediate biocontrol of diseases by interfering with pathogens or via strengthening the host. Although phytohormones, including cytokinins, are known to regulate plant development and physiology as well as plant immunity, their production by microorganisms has not been consid...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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2016
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| Acceso en línea: | http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2016grobkinsky |
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snrd:2016grobkinsky2021-10-15T16:56:07Z Grobkinsky, D. K. Tafner, R. Moreno, M. V. Stenglein, S. A. García de Salamone, I. E. Nelson, L. M. Novák, O. Strnad, M. Van der Graaff, E. Roitsch, T. 2016 Plant beneficial microbes mediate biocontrol of diseases by interfering with pathogens or via strengthening the host. Although phytohormones, including cytokinins, are known to regulate plant development and physiology as well as plant immunity, their production by microorganisms has not been considered as a biocontrol mechanism. Here we identify the ability of Pseudomonas fluorescens G20-18 to efficiently control P. syringae infection in Arabidopsis, allowing maintenance of tissue integrity and ultimately biomass yield. Microbial cytokinin production was identified as a key determinant for this biocontrol effect on the hemibiotrophic bacterial pathogen. While cytokinindeficient loss-of-function mutants of G20-18 exhibit impaired biocontrol, functional complementation with cytokinin biosynthetic genes restores cytokinin-mediated biocontrol, which is correlated with differential cytokinin levels in planta. Arabidopsis mutant analyses revealed the necessity of functional plant cytokinin perception and salicylic acid-dependent defence signalling for this biocontrol mechanism. These results demonstrate microbial cytokinin production as a novel microbebased, hormone-mediated concept of biocontrol. This mechanism provides a basis to potentially develop novel, integrated plant protection strategies combining promotion of growth, a favourable physiological status and activation of fine-tuned direct defence and abiotic stress resilience. application/pdf 10.1038/srep23310 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2016grobkinsky eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 Scientific Reports Vol.6 art.23310,11p. http://www.nature.com PSEUDOMONAS FLUORESCENS CYTOKININ PRODUCTION Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-140 |
| collection |
FAUBA Digital - Facultad de Agronomía (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
PSEUDOMONAS FLUORESCENS CYTOKININ PRODUCTION |
| spellingShingle |
PSEUDOMONAS FLUORESCENS CYTOKININ PRODUCTION Grobkinsky, D. K. Tafner, R. Moreno, M. V. Stenglein, S. A. García de Salamone, I. E. Nelson, L. M. Novák, O. Strnad, M. Van der Graaff, E. Roitsch, T. Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis |
| topic_facet |
PSEUDOMONAS FLUORESCENS CYTOKININ PRODUCTION |
| description |
Plant beneficial microbes mediate biocontrol of diseases by interfering with pathogens or via strengthening the host. Although phytohormones, including cytokinins, are known to regulate plant development and physiology as well as plant immunity, their production by microorganisms has not been considered as a biocontrol mechanism. Here we identify the ability of Pseudomonas fluorescens G20-18 to efficiently control P. syringae infection in Arabidopsis, allowing maintenance of tissue integrity and ultimately biomass yield. Microbial cytokinin production was identified as a key determinant for this biocontrol effect on the hemibiotrophic bacterial pathogen. While cytokinindeficient loss-of-function mutants of G20-18 exhibit impaired biocontrol, functional complementation with cytokinin biosynthetic genes restores cytokinin-mediated biocontrol, which is correlated with differential cytokinin levels in planta. Arabidopsis mutant analyses revealed the necessity of functional plant cytokinin perception and salicylic acid-dependent defence signalling for this biocontrol mechanism. These results demonstrate microbial cytokinin production as a novel microbebased, hormone-mediated concept of biocontrol. This mechanism provides a basis to potentially develop novel, integrated plant protection strategies combining promotion of growth, a favourable physiological status and activation of fine-tuned direct defence and abiotic stress resilience. |
| format |
Artículo Artículo publishedVersion publishedVersion |
| author |
Grobkinsky, D. K. Tafner, R. Moreno, M. V. Stenglein, S. A. García de Salamone, I. E. Nelson, L. M. Novák, O. Strnad, M. Van der Graaff, E. Roitsch, T. |
| author_facet |
Grobkinsky, D. K. Tafner, R. Moreno, M. V. Stenglein, S. A. García de Salamone, I. E. Nelson, L. M. Novák, O. Strnad, M. Van der Graaff, E. Roitsch, T. |
| author_sort |
Grobkinsky, D. K. |
| title |
Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis |
| title_short |
Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis |
| title_full |
Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis |
| title_fullStr |
Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis |
| title_full_unstemmed |
Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis |
| title_sort |
cytokinin production by pseudomonas fluorescens g20- 18 determines biocontrol activity against pseudomonas syringae in arabidopsis |
| publishDate |
2016 |
| url |
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2016grobkinsky |
| work_keys_str_mv |
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1790355001835520000 |