Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates

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Fil: Di Salvo, Luciana Paula. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.

Detalles Bibliográficos
Autores principales: Di Salvo, Luciana Paula, García de Salamone, Inés Eugenia
Formato: article Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2012
Materias:
CARBON-SOURCE UTILIZATION
COMMUNITY-LEVEL PHYSIOLOGICAL PROFILES
FUNCTIONAL DIVERSITY
METABOLIC PROFILES
ANTHROPOGENIC EFFECT
BIOASSAY
BIOINDICATOR
BIOREMEDIATION
DIVERSITY INDEX
FUNCTIONAL GROUP
LABORATORY METHOD
METABOLISM
MICROBIAL ACTIVITY
MICROBIAL COMMUNITY
NUTRIENT USE
SOIL CARBON
SOIL ECOSYSTEM
SOIL MICROORGANISM
STANDARDIZATION
TAXONOMY
Acceso en línea:http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012DiSalvo
Aporte de:
FAUBA Digital - Facultad de Agronomía (UBA) de Universidad de Buenos Aires
id snrd:2012DiSalvo
record_format dspace
spelling snrd:2012DiSalvo2021-10-15T16:56:07Z Di Salvo, Luciana Paula García de Salamone, Inés Eugenia 2012 Fil: Di Salvo, Luciana Paula. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina. Fil: García de Salamone, Inés Eugenia. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina. Variation of soil-microbial communities are good bioindicators of human impacts in soils, such as different soils management or contamination. Considering that traditional methods of isolation and taxonomic analysis do not consider the functionality of the microbial community, Community-Level Physiological Profiles (CLPP) emerged as a complementary methodology to study microbial communities. Several studies have shown that Biolog® EcoPlates® are very useful for determining physiological differences between communities from different samples. However, commercial microplates have some disadvantages which led us to the idea of replacing them by microplates prepared in the laboratory (Laboratory's). Here, we compared both types of microplates using soil samples from a bioremediation assay. We compared a) the average well color development for each sample, b) the averages of absorbance values for each type of microplate, c) Principal Components, and d) Shannon-Weaver's diversity index (H). Although Laboratory's showed significantly lower Average absorbance values than EcoPlates®, the principal component analysis and diversity index did not differ between types of microplates. In conclusion, both types of microplates showed a relatively similar ability to detect differences in the CLPP of the treatments studied. Consequently, microplates prepared in laboratory are a reliable and economical tool to study the physiology of soil microbial communities. application/pdf issn:0327-5477 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012DiSalvo eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 Ecología austral Vol.22, no.2 129-136 https://asaeargentina.com.ar CARBON-SOURCE UTILIZATION COMMUNITY-LEVEL PHYSIOLOGICAL PROFILES FUNCTIONAL DIVERSITY METABOLIC PROFILES ANTHROPOGENIC EFFECT BIOASSAY BIOINDICATOR BIOREMEDIATION DIVERSITY INDEX FUNCTIONAL GROUP LABORATORY METHOD METABOLISM MICROBIAL ACTIVITY MICROBIAL COMMUNITY NUTRIENT USE SOIL CARBON SOIL ECOSYSTEM SOIL MICROORGANISM STANDARDIZATION TAXONOMY Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates article info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-140
collection FAUBA Digital - Facultad de Agronomía (UBA)
language Inglés
orig_language_str_mv eng
topic CARBON-SOURCE UTILIZATION
COMMUNITY-LEVEL PHYSIOLOGICAL PROFILES
FUNCTIONAL DIVERSITY
METABOLIC PROFILES
ANTHROPOGENIC EFFECT
BIOASSAY
BIOINDICATOR
BIOREMEDIATION
DIVERSITY INDEX
FUNCTIONAL GROUP
LABORATORY METHOD
METABOLISM
MICROBIAL ACTIVITY
MICROBIAL COMMUNITY
NUTRIENT USE
SOIL CARBON
SOIL ECOSYSTEM
SOIL MICROORGANISM
STANDARDIZATION
TAXONOMY
spellingShingle CARBON-SOURCE UTILIZATION
COMMUNITY-LEVEL PHYSIOLOGICAL PROFILES
FUNCTIONAL DIVERSITY
METABOLIC PROFILES
ANTHROPOGENIC EFFECT
BIOASSAY
BIOINDICATOR
BIOREMEDIATION
DIVERSITY INDEX
FUNCTIONAL GROUP
LABORATORY METHOD
METABOLISM
MICROBIAL ACTIVITY
MICROBIAL COMMUNITY
NUTRIENT USE
SOIL CARBON
SOIL ECOSYSTEM
SOIL MICROORGANISM
STANDARDIZATION
TAXONOMY
Di Salvo, Luciana Paula
García de Salamone, Inés Eugenia
Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates
topic_facet CARBON-SOURCE UTILIZATION
COMMUNITY-LEVEL PHYSIOLOGICAL PROFILES
FUNCTIONAL DIVERSITY
METABOLIC PROFILES
ANTHROPOGENIC EFFECT
BIOASSAY
BIOINDICATOR
BIOREMEDIATION
DIVERSITY INDEX
FUNCTIONAL GROUP
LABORATORY METHOD
METABOLISM
MICROBIAL ACTIVITY
MICROBIAL COMMUNITY
NUTRIENT USE
SOIL CARBON
SOIL ECOSYSTEM
SOIL MICROORGANISM
STANDARDIZATION
TAXONOMY
description Fil: Di Salvo, Luciana Paula. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.
format article
Artículo
Artículo
publishedVersion
publishedVersion
author Di Salvo, Luciana Paula
García de Salamone, Inés Eugenia
author_facet Di Salvo, Luciana Paula
García de Salamone, Inés Eugenia
author_sort Di Salvo, Luciana Paula
title Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates
title_short Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates
title_full Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates
title_fullStr Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates
title_full_unstemmed Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates
title_sort evaluation of soil-microbial communities by their clpp : standardization of a laboratory technique to replace commercial available microplates
publishDate 2012
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012DiSalvo
work_keys_str_mv AT disalvolucianapaula evaluationofsoilmicrobialcommunitiesbytheirclppstandardizationofalaboratorytechniquetoreplacecommercialavailablemicroplates
AT garciadesalamoneineseugenia evaluationofsoilmicrobialcommunitiesbytheirclppstandardizationofalaboratorytechniquetoreplacecommercialavailablemicroplates
_version_ 1827450788807442432

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