Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine

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Fil: Salvador, I. Centro de Tecnología Animal. Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA). Apdo. 187, 12400 Segorbe (Castellón), Spain.

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Autores principales: Salvador, I., Cebrian-Serrano, A., Salamone, Daniel Felipe, Silvestre, M. A.
Formato: article Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2011
Materias:
LOW EMBRYO CULTURE
SINGLE
BOVINAE
Acceso en línea:http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2011Salvador
Aporte de:
FAUBA Digital - Facultad de Agronomía (UBA) de Universidad de Buenos Aires
id snrd:2011Salvador
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spelling snrd:2011Salvador2021-10-15T16:56:07Z Salvador, I. Cebrian-Serrano, A. Salamone, Daniel Felipe Silvestre, M. A. 2011 Fil: Salvador, I. Centro de Tecnología Animal. Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA). Apdo. 187, 12400 Segorbe (Castellón), Spain. Fil: Cebrian-Serrano, A. Centro de Tecnología Animal. Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA). Apdo. 187, 12400 Segorbe (Castellón), Spain. Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal. Buenos Aires, Argentina. Fil: Silvestre, M. A. Centro de Tecnología Animal. Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA). Apdo. 187, 12400 Segorbe (Castellón), Spain. The aim of this study was to identify the in vitro development stage at which the culture of a single or low number (n = 5 or 10) of oocytes/embryos could impair development in comparison with culture in group (n = 50). In the Experiment 1, it was confirmed that single in vitro embryo production yielded lower cleavage and blastocyst rates than in group (49.4 vs. 83.0 percent; 0 percent vs. 37.8 percent, respectively; p less than 0.05). In Experiment 2 and 3, it was observed no effect on embryo development of culturing single or low number of oocytes during maturation and fertilization, respectively. In Experiment 4, it was observed a detrimental effect on blastocyst rate when cultured single or low number of embryos during post-fertilization in vitro culture (2.9; 10.2-10.8; 33.2 percent in single, low number of embryos (5-10), and controlgrouped, respectively; p less than 0.05). In Experiment 5, it was observed that the last part of the culture period (day 3 onwards) seemed to be more affected by the low number of embryos placed in culture. In conclusion, post-fertilization culture, especially on days 3 to 7 after fertilization, seems to be the most important stage for embryo development on single and/or low number (5-10) of embryos culture. application/pdf issn:1695-971X http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2011Salvador eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 Spanish Journal of Agricultural Research Vol.9, no.3 744-752 http://www.inia.es/ LOW EMBRYO CULTURE SINGLE BOVINAE Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine article info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-140
collection FAUBA Digital - Facultad de Agronomía (UBA)
language Inglés
orig_language_str_mv eng
topic LOW EMBRYO CULTURE
SINGLE
BOVINAE
spellingShingle LOW EMBRYO CULTURE
SINGLE
BOVINAE
Salvador, I.
Cebrian-Serrano, A.
Salamone, Daniel Felipe
Silvestre, M. A.
Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine
topic_facet LOW EMBRYO CULTURE
SINGLE
BOVINAE
description Fil: Salvador, I. Centro de Tecnología Animal. Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA). Apdo. 187, 12400 Segorbe (Castellón), Spain.
format article
Artículo
Artículo
publishedVersion
publishedVersion
author Salvador, I.
Cebrian-Serrano, A.
Salamone, Daniel Felipe
Silvestre, M. A.
author_facet Salvador, I.
Cebrian-Serrano, A.
Salamone, Daniel Felipe
Silvestre, M. A.
author_sort Salvador, I.
title Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine
title_short Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine
title_full Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine
title_fullStr Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine
title_full_unstemmed Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine
title_sort effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine
publishDate 2011
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2011Salvador
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AT silvestrema effectofnumberofoocytesandembryosoninvitrooocytematurationfertilizationandembryodevelopmentinbovine
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