Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol

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Fil: Kronberg, María Florencia. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología. Buenos Aires, Argentina.

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Autores principales:	Galvagno, Miguel Angel, Iannone, Leopoldo J., Bianchi, Jorgelina, Kronberg, María Florencia, Rost, Enrique, Carstens, María R., Cerrutti, Patricia
Formato:	article Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2011
Materias:	FATTY ACIDS GLYCEROL LIPASE YARROWIA LIPOLYTICA MUTANT BIODIESEL CARBON FATTY ACID OLIVE OIL ORGANIC NITROGEN TRIACYLGLYCEROL LIPASE BATCH FERMENTATION BATCH REACTOR BIOMASS PRODUCTION CONTROLLED STUDY ENZYME ACTIVITY ENZYME SYNTHESIS FERMENTATION OPTIMIZATION FUNGUS MUTANT NONHUMAN PROCESS OPTIMIZATION RESPONSE SURFACE METHOD YARROWIA LIPOLYTICA BIOFUELS BIOMASS BIOREACTORS CULTURE MEDIA CULTURE MEDIA, CONDITIONED DNA, FUNGAL DNA, INTERGENIC FERMENTATION FUNGAL PROTEINS GENES, FUNGAL HYPHAE INDUSTRIAL MICROBIOLOGY MYCOLOGY YARROWIA
Acceso en línea:	http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2011Galvagno
Aporte de:	FAUBA Digital - Facultad de Agronomía (UBA) de Universidad de Buenos Aires

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spelling	snrd:2011Galvagno2021-10-15T16:56:07Z Galvagno, Miguel Angel Iannone, Leopoldo J. Bianchi, Jorgelina Kronberg, María Florencia Rost, Enrique Carstens, María R. Cerrutti, Patricia 2011 Fil: Kronberg, María Florencia. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología. Buenos Aires, Argentina. Fil: Kronberg, María Florencia. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. Fil: Kronberg, María Florencia. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. The yeast Yarrowia lipolytica accumulates oils and is able to produce extracellular lipases when growing in different carbon sources including glycerol, the principal by-product of the biodiesel industry. In this study, biomass production of a novel mutant strain of Y. lipolytica was statistically optimized by Response Surface Methodology in media containing biodiesel-derived glycerol as main carbon source. This strain exhibited distinctive morphological and fatty acid profile characteristics, and showed an increased extracellular lipase activity. An organic source of nitrogen and the addition of 1.0 g/l olive oil were necessary for significant lipase production. Plackett-Burman and Central Composite Statistical Designs were employed for screening and optimization of fermentation in shaken flasks cultures, and the maximum values obtained were 16.1 g/l for biomass and 12.2 Units/ml for lipase, respectively. Optimized batch bioprocess was thereafter scaled in aerated bioreactors and the values reached for lipase specific activity after 95 percent of the glycerol had been consumed, were three-fold higher than those obtained in shaken flasks cultures. A sustainable bioprocess to obtain biomass and extracellular lipase activity was attained by maximizing the use of the by-products of biodiesel industry. application/pdf issn:0325-7541 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2011Galvagno eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 Revista argentina de microbiología Vol.43, no.3 218-225 http://www.aam.org.ar/ FATTY ACIDS GLYCEROL LIPASE YARROWIA LIPOLYTICA MUTANT BIODIESEL CARBON FATTY ACID OLIVE OIL ORGANIC NITROGEN TRIACYLGLYCEROL LIPASE BATCH FERMENTATION BATCH REACTOR BIOMASS PRODUCTION CONTROLLED STUDY ENZYME ACTIVITY ENZYME SYNTHESIS FERMENTATION OPTIMIZATION FUNGUS MUTANT NONHUMAN PROCESS OPTIMIZATION RESPONSE SURFACE METHOD YARROWIA LIPOLYTICA BIOFUELS BIOMASS BIOREACTORS CULTURE MEDIA CULTURE MEDIA, CONDITIONED DNA, FUNGAL DNA, INTERGENIC FERMENTATION FUNGAL PROTEINS GENES, FUNGAL HYPHAE INDUSTRIAL MICROBIOLOGY MYCOLOGY YARROWIA Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol article info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-140
collection	FAUBA Digital - Facultad de Agronomía (UBA)
language	Inglés
orig_language_str_mv	eng
topic	FATTY ACIDS GLYCEROL LIPASE YARROWIA LIPOLYTICA MUTANT BIODIESEL CARBON FATTY ACID OLIVE OIL ORGANIC NITROGEN TRIACYLGLYCEROL LIPASE BATCH FERMENTATION BATCH REACTOR BIOMASS PRODUCTION CONTROLLED STUDY ENZYME ACTIVITY ENZYME SYNTHESIS FERMENTATION OPTIMIZATION FUNGUS MUTANT NONHUMAN PROCESS OPTIMIZATION RESPONSE SURFACE METHOD YARROWIA LIPOLYTICA BIOFUELS BIOMASS BIOREACTORS CULTURE MEDIA CULTURE MEDIA, CONDITIONED DNA, FUNGAL DNA, INTERGENIC FERMENTATION FUNGAL PROTEINS GENES, FUNGAL HYPHAE INDUSTRIAL MICROBIOLOGY MYCOLOGY YARROWIA
spellingShingle	FATTY ACIDS GLYCEROL LIPASE YARROWIA LIPOLYTICA MUTANT BIODIESEL CARBON FATTY ACID OLIVE OIL ORGANIC NITROGEN TRIACYLGLYCEROL LIPASE BATCH FERMENTATION BATCH REACTOR BIOMASS PRODUCTION CONTROLLED STUDY ENZYME ACTIVITY ENZYME SYNTHESIS FERMENTATION OPTIMIZATION FUNGUS MUTANT NONHUMAN PROCESS OPTIMIZATION RESPONSE SURFACE METHOD YARROWIA LIPOLYTICA BIOFUELS BIOMASS BIOREACTORS CULTURE MEDIA CULTURE MEDIA, CONDITIONED DNA, FUNGAL DNA, INTERGENIC FERMENTATION FUNGAL PROTEINS GENES, FUNGAL HYPHAE INDUSTRIAL MICROBIOLOGY MYCOLOGY YARROWIA Galvagno, Miguel Angel Iannone, Leopoldo J. Bianchi, Jorgelina Kronberg, María Florencia Rost, Enrique Carstens, María R. Cerrutti, Patricia Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol
topic_facet	FATTY ACIDS GLYCEROL LIPASE YARROWIA LIPOLYTICA MUTANT BIODIESEL CARBON FATTY ACID OLIVE OIL ORGANIC NITROGEN TRIACYLGLYCEROL LIPASE BATCH FERMENTATION BATCH REACTOR BIOMASS PRODUCTION CONTROLLED STUDY ENZYME ACTIVITY ENZYME SYNTHESIS FERMENTATION OPTIMIZATION FUNGUS MUTANT NONHUMAN PROCESS OPTIMIZATION RESPONSE SURFACE METHOD YARROWIA LIPOLYTICA BIOFUELS BIOMASS BIOREACTORS CULTURE MEDIA CULTURE MEDIA, CONDITIONED DNA, FUNGAL DNA, INTERGENIC FERMENTATION FUNGAL PROTEINS GENES, FUNGAL HYPHAE INDUSTRIAL MICROBIOLOGY MYCOLOGY YARROWIA
description	Fil: Kronberg, María Florencia. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología. Buenos Aires, Argentina.
format	article Artículo Artículo publishedVersion publishedVersion
author	Galvagno, Miguel Angel Iannone, Leopoldo J. Bianchi, Jorgelina Kronberg, María Florencia Rost, Enrique Carstens, María R. Cerrutti, Patricia
author_facet	Galvagno, Miguel Angel Iannone, Leopoldo J. Bianchi, Jorgelina Kronberg, María Florencia Rost, Enrique Carstens, María R. Cerrutti, Patricia
author_sort	Galvagno, Miguel Angel
title	Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol
title_short	Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol
title_full	Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol
title_fullStr	Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol
title_full_unstemmed	Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol
title_sort	optimization of biomass production of a mutant of yarrowia lipolytica with an increased lipase activity using raw glycerol
publishDate	2011
url	http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2011Galvagno
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Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol

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