AHAS herbicide resistance endowing mutations : Effect on AHAS functionality and plant growth

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Fil: Yu, Qin. University of Western Australia (UWA). School of Agriculture and Environment. Australian Herbicide Resistance Initiative (AHRI). Crawley, Western Australia, Australia.

Detalles Bibliográficos
Autores principales: Yu, Qin, Han, Heping, Vila Aiub, Martín Miguel, Powles, Stephen B.
Formato: article Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2010
Materias:
AHAS RESISTANCE MUTATION
ENZYME KINETICS
HERBICIDE RESISTANCE
RELATIVE GROWTH RATE
ACETOLACTATE SYNTHASE
BRANCHED CHAIN AMINO ACID
HERBICIDE
DRUG ANTAGONISM
DRUG EFFECT
ENZYMOLOGY
GENETICS
GROWTH, DEVELOPMENT AND AGING
HOMOZYGOTE
LOLIUM
METABOLISM
MUTATION
AMINO ACID SUBSTITUTION
AMINO ACIDS, BRANCHED-CHAIN
HERBICIDES
LOLIUM RIGIDUM
Acceso en línea:http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2010Yu
Aporte de:
FAUBA Digital - Facultad de Agronomía (UBA) de Universidad de Buenos Aires
id snrd:2010Yu
record_format dspace
spelling snrd:2010Yu2021-10-15T16:56:07Z Yu, Qin Han, Heping Vila Aiub, Martín Miguel Powles, Stephen B. 2010 Fil: Yu, Qin. University of Western Australia (UWA). School of Agriculture and Environment. Australian Herbicide Resistance Initiative (AHRI). Crawley, Western Australia, Australia. Fil: Han, Heping. University of Western Australia (UWA). School of Agriculture and Environment. Australian Herbicide Resistance Initiative (AHRI). Crawley, Western Australia, Australia. Fil: Vila Aiub, Martín Miguel. University of Western Australia (UWA). School of Agriculture and Environment. Australian Herbicide Resistance Initiative (AHRI). Crawley, Western Australia, Australia. Fil: Vila Aiub, Martín Miguel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina. Fil: Vila Aiub, Martín Miguel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina. Fil: Powles, Stephen B. University of Western Australia (UWA). School of Agriculture and Environment. Australian Herbicide Resistance Initiative (AHRI). Crawley, Western Australia, Australia. Twenty-two amino acid substitutions at seven conserved amino acid residues in the acetohydroxyacid synthase (AHAS) gene have been identified to date that confer target-site resistance to AHAS-inhibiting herbicides in biotypes of field-evolved resistant weed species. However, the effect of resistance mutations on AHAS functionality and plant growth has been investigated for only a very few mutations. This research investigates the effect of various AHAS resistance mutations in Lolium rigidum on AHAS functionality and plant growth. The enzyme kinetics of AHAS from five purified L. rigidum populations, each homozygous for the resistance mutations Pro-197-Ala, Pro-197-Arg, Pro-197-Gln, Pro-197-Ser or Trp-574-Leu, were characterized and the pleiotropic effect of three mutations on plant growth was assessed via relative growth rate analysis. All these resistance mutations endowed a herbicide-resistant AHAS and most resulted in higher extractable AHAS activity, with no-to-minor changes in AHAS kinetics. The Pro-197-Arg mutation slightly (but significantly) increased the K m for pyruvate and remarkably increased sensitivity to feedback inhibition by branched chain amino acids. Whereas the Pro-197-Ser and Trp-574-Leu mutations exhibited no significant effects on plant growth, the Pro-197-Arg mutation resulted in lower growth rates. It is clear that, at least in L. rigidum, these five AHAS resistance mutations have no major impact on AHAS functionality and hence probably no plant resistance costs. These results, in part, explain why so many Pro-197 AHAS resistance mutations in AHAS have evolved and why the Pro-197-Ser and the Trp-574-Leu AHAS resistance mutations are frequently found in many weed species. application/pdf doi:10.1093/jxb/erq205 issn:0022-0957 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2010Yu eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 Journal of Experimental Botany Vol.61, no.14 3925-3934 https://academic.oup.com/jxb AHAS RESISTANCE MUTATION ENZYME KINETICS HERBICIDE RESISTANCE RELATIVE GROWTH RATE ACETOLACTATE SYNTHASE BRANCHED CHAIN AMINO ACID HERBICIDE DRUG ANTAGONISM DRUG EFFECT ENZYMOLOGY GENETICS GROWTH, DEVELOPMENT AND AGING HERBICIDE RESISTANCE HOMOZYGOTE LOLIUM METABOLISM MUTATION ACETOLACTATE SYNTHASE AMINO ACID SUBSTITUTION AMINO ACIDS, BRANCHED-CHAIN HERBICIDES HOMOZYGOTE LOLIUM RIGIDUM AHAS herbicide resistance endowing mutations : Effect on AHAS functionality and plant growth article info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-140
collection FAUBA Digital - Facultad de Agronomía (UBA)
language Inglés
orig_language_str_mv eng
topic AHAS RESISTANCE MUTATION
ENZYME KINETICS
HERBICIDE RESISTANCE
RELATIVE GROWTH RATE
ACETOLACTATE SYNTHASE
BRANCHED CHAIN AMINO ACID
HERBICIDE
DRUG ANTAGONISM
DRUG EFFECT
ENZYMOLOGY
GENETICS
GROWTH, DEVELOPMENT AND AGING
HERBICIDE RESISTANCE
HOMOZYGOTE
LOLIUM
METABOLISM
MUTATION
ACETOLACTATE SYNTHASE
AMINO ACID SUBSTITUTION
AMINO ACIDS, BRANCHED-CHAIN
HERBICIDES
HOMOZYGOTE
LOLIUM RIGIDUM
spellingShingle AHAS RESISTANCE MUTATION
ENZYME KINETICS
HERBICIDE RESISTANCE
RELATIVE GROWTH RATE
ACETOLACTATE SYNTHASE
BRANCHED CHAIN AMINO ACID
HERBICIDE
DRUG ANTAGONISM
DRUG EFFECT
ENZYMOLOGY
GENETICS
GROWTH, DEVELOPMENT AND AGING
HERBICIDE RESISTANCE
HOMOZYGOTE
LOLIUM
METABOLISM
MUTATION
ACETOLACTATE SYNTHASE
AMINO ACID SUBSTITUTION
AMINO ACIDS, BRANCHED-CHAIN
HERBICIDES
HOMOZYGOTE
LOLIUM RIGIDUM
Yu, Qin
Han, Heping
Vila Aiub, Martín Miguel
Powles, Stephen B.
AHAS herbicide resistance endowing mutations : Effect on AHAS functionality and plant growth
topic_facet AHAS RESISTANCE MUTATION
ENZYME KINETICS
HERBICIDE RESISTANCE
RELATIVE GROWTH RATE
ACETOLACTATE SYNTHASE
BRANCHED CHAIN AMINO ACID
HERBICIDE
DRUG ANTAGONISM
DRUG EFFECT
ENZYMOLOGY
GENETICS
GROWTH, DEVELOPMENT AND AGING
HERBICIDE RESISTANCE
HOMOZYGOTE
LOLIUM
METABOLISM
MUTATION
ACETOLACTATE SYNTHASE
AMINO ACID SUBSTITUTION
AMINO ACIDS, BRANCHED-CHAIN
HERBICIDES
HOMOZYGOTE
LOLIUM RIGIDUM
description Fil: Yu, Qin. University of Western Australia (UWA). School of Agriculture and Environment. Australian Herbicide Resistance Initiative (AHRI). Crawley, Western Australia, Australia.
format article
Artículo
Artículo
publishedVersion
publishedVersion
author Yu, Qin
Han, Heping
Vila Aiub, Martín Miguel
Powles, Stephen B.
author_facet Yu, Qin
Han, Heping
Vila Aiub, Martín Miguel
Powles, Stephen B.
author_sort Yu, Qin
title AHAS herbicide resistance endowing mutations : Effect on AHAS functionality and plant growth
title_short AHAS herbicide resistance endowing mutations : Effect on AHAS functionality and plant growth
title_full AHAS herbicide resistance endowing mutations : Effect on AHAS functionality and plant growth
title_fullStr AHAS herbicide resistance endowing mutations : Effect on AHAS functionality and plant growth
title_full_unstemmed AHAS herbicide resistance endowing mutations : Effect on AHAS functionality and plant growth
title_sort ahas herbicide resistance endowing mutations : effect on ahas functionality and plant growth
publishDate 2010
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2010Yu
work_keys_str_mv AT yuqin ahasherbicideresistanceendowingmutationseffectonahasfunctionalityandplantgrowth
AT hanheping ahasherbicideresistanceendowingmutationseffectonahasfunctionalityandplantgrowth
AT vilaaiubmartinmiguel ahasherbicideresistanceendowingmutationseffectonahasfunctionalityandplantgrowth
AT powlesstephenb ahasherbicideresistanceendowingmutationseffectonahasfunctionalityandplantgrowth
_version_ 1827450830194737152

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