Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation

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The mammalian sperm nucleus contains an unusually condensed chromatin, due to replacement of the majority of histones by protamines. However, soon after the spermatozoon penetrates the ooplasm at fertilization, decondensation of this densely packed chromatin must occur to allow formation of the male...

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Detalles Bibliográficos
Autores principales: Sanchez, M.C., Alvarez Sedo, C., Julianelli, V.L., Romanato, M., Calvo, L., Calvo, J.C., Fontana, V.A.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2013
Materias:
Chromatin decondensation
Dermatan sulfate
Heparin
Murine spermatozoa
Synergism
dermatan sulfate
glutathione
glycosaminoglycan
heparin
animal cell
article
chromatin
chromatin decondensation'
controlled study
dose response
in vitro study
male
mouse
nonhuman
phase contrast microscopy
priority journal
spermatozoon
transmission electron microscopy
Animals
Chromatin
Dermatan Sulfate
Dose-Response Relationship, Drug
Male
Mice
Microscopy, Electron
Spermatozoa
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19396368_v59_n2_p82_Sanchez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paperaa:paper_19396368_v59_n2_p82_Sanchez2023-06-12T16:51:52Z Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation Syst. Biology Reprod. Med. 2013;59(2):82-90 Sanchez, M.C. Alvarez Sedo, C. Julianelli, V.L. Romanato, M. Calvo, L. Calvo, J.C. Fontana, V.A. Chromatin decondensation Dermatan sulfate Heparin Murine spermatozoa Synergism dermatan sulfate glutathione glycosaminoglycan heparin animal cell article chromatin chromatin decondensation' controlled study dose response in vitro study male mouse nonhuman phase contrast microscopy priority journal spermatozoon transmission electron microscopy Animals Chromatin Dermatan Sulfate Dose-Response Relationship, Drug Heparin Male Mice Microscopy, Electron Spermatozoa The mammalian sperm nucleus contains an unusually condensed chromatin, due to replacement of the majority of histones by protamines. However, soon after the spermatozoon penetrates the ooplasm at fertilization, decondensation of this densely packed chromatin must occur to allow formation of the male pronucleus and syngamy. Decondensation is accomplished by protamine disulfide bond reduction by oocyte glutathione and replacement of protamines by oocyte histones with the aid of an acceptor molecule. Previous results from our laboratory have demonstrated that heparan sulfate (HS) present in the ooplasm functions as protamine acceptor during human sperm decondensation in vivo. In the present paper, we analyze the role of heparin, structural analogue of HS, and dermatan sulfate (DS) in murine sperm chromatin decondensation in vitro, including the possibility of a synergistic effect between both glycosaminoglycans. Decondensation was assessed under phase contrast microscopy following incubation of murine spermatozoa with glutathione and either heparin, DS, or a combination of both. Ultrastructural changes taking place during decondensation were analyzed by transmission electron microscopy. Both glycosaminoglycans were able to promote the decondensation of murine spermatozoa in vitro but the decondensing ability of heparin was significantly higher. Use of both glycosaminoglycans together revealed the existence of a synergistic effect. Transmission electron microscopy analysis of decondensing spermatozoa supported these findings. Synergism between heparin and DS was observed both in capacitated and non-capacitated spermatozoa but decondensation kinetics was faster in the former. The results obtained indicate a new potential role for dermatan sulfate in murine sperm decondensation at fertilization and provide evidence of differences in the degree of chromatin condensation throughout the murine sperm nucleus. © 2013 Informa Healthcare USA, Inc. Fil:Julianelli, V.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romanato, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fontana, V.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19396368_v59_n2_p82_Sanchez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic Chromatin decondensation
Dermatan sulfate
Heparin
Murine spermatozoa
Synergism
dermatan sulfate
glutathione
glycosaminoglycan
heparin
animal cell
article
chromatin
chromatin decondensation'
controlled study
dose response
in vitro study
male
mouse
nonhuman
phase contrast microscopy
priority journal
spermatozoon
transmission electron microscopy
Animals
Chromatin
Dermatan Sulfate
Dose-Response Relationship, Drug
Heparin
Male
Mice
Microscopy, Electron
Spermatozoa
spellingShingle Chromatin decondensation
Dermatan sulfate
Heparin
Murine spermatozoa
Synergism
dermatan sulfate
glutathione
glycosaminoglycan
heparin
animal cell
article
chromatin
chromatin decondensation'
controlled study
dose response
in vitro study
male
mouse
nonhuman
phase contrast microscopy
priority journal
spermatozoon
transmission electron microscopy
Animals
Chromatin
Dermatan Sulfate
Dose-Response Relationship, Drug
Heparin
Male
Mice
Microscopy, Electron
Spermatozoa
Sanchez, M.C.
Alvarez Sedo, C.
Julianelli, V.L.
Romanato, M.
Calvo, L.
Calvo, J.C.
Fontana, V.A.
Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
topic_facet Chromatin decondensation
Dermatan sulfate
Heparin
Murine spermatozoa
Synergism
dermatan sulfate
glutathione
glycosaminoglycan
heparin
animal cell
article
chromatin
chromatin decondensation'
controlled study
dose response
in vitro study
male
mouse
nonhuman
phase contrast microscopy
priority journal
spermatozoon
transmission electron microscopy
Animals
Chromatin
Dermatan Sulfate
Dose-Response Relationship, Drug
Heparin
Male
Mice
Microscopy, Electron
Spermatozoa
description The mammalian sperm nucleus contains an unusually condensed chromatin, due to replacement of the majority of histones by protamines. However, soon after the spermatozoon penetrates the ooplasm at fertilization, decondensation of this densely packed chromatin must occur to allow formation of the male pronucleus and syngamy. Decondensation is accomplished by protamine disulfide bond reduction by oocyte glutathione and replacement of protamines by oocyte histones with the aid of an acceptor molecule. Previous results from our laboratory have demonstrated that heparan sulfate (HS) present in the ooplasm functions as protamine acceptor during human sperm decondensation in vivo. In the present paper, we analyze the role of heparin, structural analogue of HS, and dermatan sulfate (DS) in murine sperm chromatin decondensation in vitro, including the possibility of a synergistic effect between both glycosaminoglycans. Decondensation was assessed under phase contrast microscopy following incubation of murine spermatozoa with glutathione and either heparin, DS, or a combination of both. Ultrastructural changes taking place during decondensation were analyzed by transmission electron microscopy. Both glycosaminoglycans were able to promote the decondensation of murine spermatozoa in vitro but the decondensing ability of heparin was significantly higher. Use of both glycosaminoglycans together revealed the existence of a synergistic effect. Transmission electron microscopy analysis of decondensing spermatozoa supported these findings. Synergism between heparin and DS was observed both in capacitated and non-capacitated spermatozoa but decondensation kinetics was faster in the former. The results obtained indicate a new potential role for dermatan sulfate in murine sperm decondensation at fertilization and provide evidence of differences in the degree of chromatin condensation throughout the murine sperm nucleus. © 2013 Informa Healthcare USA, Inc.
format Artículo
Artículo
publishedVersion
author Sanchez, M.C.
Alvarez Sedo, C.
Julianelli, V.L.
Romanato, M.
Calvo, L.
Calvo, J.C.
Fontana, V.A.
author_facet Sanchez, M.C.
Alvarez Sedo, C.
Julianelli, V.L.
Romanato, M.
Calvo, L.
Calvo, J.C.
Fontana, V.A.
author_sort Sanchez, M.C.
title Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
title_short Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
title_full Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
title_fullStr Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
title_full_unstemmed Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
title_sort dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
publishDate 2013
url http://hdl.handle.net/20.500.12110/paper_19396368_v59_n2_p82_Sanchez
work_keys_str_mv AT sanchezmc dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation
AT alvarezsedoc dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation
AT julianellivl dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation
AT romanatom dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation
AT calvol dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation
AT calvojc dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation
AT fontanava dermatansulfatesynergizeswithheparininmurinespermchromatindecondensation
_version_ 1769810148234100736

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