Transport Properties of Melanosomes along Microtubules Interpreted by a Tug-of-War Model with Loose Mechanical Coupling
In this work, we explored theoretically the transport of organelles driven along microtubules by molecular motors of opposed polarities using a stochastic model that considers a Langevin dynamics for the cargo, independent cargo-motor linkers and stepping motion for the motors. It has been recently...
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paperaa:paper_19326203_v7_n8_p_Bouzat2023-06-12T16:51:42Z Transport Properties of Melanosomes along Microtubules Interpreted by a Tug-of-War Model with Loose Mechanical Coupling PLoS ONE 2012;7(8) Bouzat, S. Levi, V. Bruno, L. molecular motor article cell adhesion cell aggregation cell motility cell stimulation cellular parameters controlled study dispersion in vitro study intracellular transport mechanical coupling melanophore melanosome microtubule nonhuman stochastic model theoretical model tug of war model velocity Xenopus laevis Animals Biological Transport Biomechanics Kinetics Mechanical Processes Melanosomes Microtubules Models, Biological Molecular Motor Proteins Movement Stochastic Processes Xenopus laevis Xenopus laevis In this work, we explored theoretically the transport of organelles driven along microtubules by molecular motors of opposed polarities using a stochastic model that considers a Langevin dynamics for the cargo, independent cargo-motor linkers and stepping motion for the motors. It has been recently proposed that the stiffness of the motor plays an important role when multiple motors collectively transport a cargo. Therefore, we considered in our model the recently reported values for the stiffness of the cargo-motor linker determined in living cells (~0.01 pN/nm, [1]) which is significantly lower than the motor stiffness obtained in in vitro assays and used in previous studies. Our model could reproduce the multimodal velocity distributions and typical trajectory characteristics including the properties of the reversions in the overall direction of motion observed during melanosome transport along microtubules in Xenopus laevis melanophores. Moreover, we explored the contribution of the different motility states of the cargo-motor system to the different modes of the velocity distributions and could identify the microscopic mechanisms of transport leading to trajectories compatible with those observed in living cells. Finally, by changing the attachment and detachment rates, the model could reproduce the different velocity distributions observed during melanosome transport along microtubules in Xenopus laevis melanophores stimulated for aggregation and dispersion. Our analysis suggests that active tug-of-war processes with loose mechanical coupling can account for several aspects of cargo transport along microtubules in living cells. © 2012 Bouzat et al. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v7_n8_p_Bouzat |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
language |
Inglés |
orig_language_str_mv |
eng |
topic |
molecular motor article cell adhesion cell aggregation cell motility cell stimulation cellular parameters controlled study dispersion in vitro study intracellular transport mechanical coupling melanophore melanosome microtubule nonhuman stochastic model theoretical model tug of war model velocity Xenopus laevis Animals Biological Transport Biomechanics Kinetics Mechanical Processes Melanosomes Microtubules Models, Biological Molecular Motor Proteins Movement Stochastic Processes Xenopus laevis Xenopus laevis |
spellingShingle |
molecular motor article cell adhesion cell aggregation cell motility cell stimulation cellular parameters controlled study dispersion in vitro study intracellular transport mechanical coupling melanophore melanosome microtubule nonhuman stochastic model theoretical model tug of war model velocity Xenopus laevis Animals Biological Transport Biomechanics Kinetics Mechanical Processes Melanosomes Microtubules Models, Biological Molecular Motor Proteins Movement Stochastic Processes Xenopus laevis Xenopus laevis Bouzat, S. Levi, V. Bruno, L. Transport Properties of Melanosomes along Microtubules Interpreted by a Tug-of-War Model with Loose Mechanical Coupling |
topic_facet |
molecular motor article cell adhesion cell aggregation cell motility cell stimulation cellular parameters controlled study dispersion in vitro study intracellular transport mechanical coupling melanophore melanosome microtubule nonhuman stochastic model theoretical model tug of war model velocity Xenopus laevis Animals Biological Transport Biomechanics Kinetics Mechanical Processes Melanosomes Microtubules Models, Biological Molecular Motor Proteins Movement Stochastic Processes Xenopus laevis Xenopus laevis |
description |
In this work, we explored theoretically the transport of organelles driven along microtubules by molecular motors of opposed polarities using a stochastic model that considers a Langevin dynamics for the cargo, independent cargo-motor linkers and stepping motion for the motors. It has been recently proposed that the stiffness of the motor plays an important role when multiple motors collectively transport a cargo. Therefore, we considered in our model the recently reported values for the stiffness of the cargo-motor linker determined in living cells (~0.01 pN/nm, [1]) which is significantly lower than the motor stiffness obtained in in vitro assays and used in previous studies. Our model could reproduce the multimodal velocity distributions and typical trajectory characteristics including the properties of the reversions in the overall direction of motion observed during melanosome transport along microtubules in Xenopus laevis melanophores. Moreover, we explored the contribution of the different motility states of the cargo-motor system to the different modes of the velocity distributions and could identify the microscopic mechanisms of transport leading to trajectories compatible with those observed in living cells. Finally, by changing the attachment and detachment rates, the model could reproduce the different velocity distributions observed during melanosome transport along microtubules in Xenopus laevis melanophores stimulated for aggregation and dispersion. Our analysis suggests that active tug-of-war processes with loose mechanical coupling can account for several aspects of cargo transport along microtubules in living cells. © 2012 Bouzat et al. |
format |
Artículo Artículo publishedVersion |
author |
Bouzat, S. Levi, V. Bruno, L. |
author_facet |
Bouzat, S. Levi, V. Bruno, L. |
author_sort |
Bouzat, S. |
title |
Transport Properties of Melanosomes along Microtubules Interpreted by a Tug-of-War Model with Loose Mechanical Coupling |
title_short |
Transport Properties of Melanosomes along Microtubules Interpreted by a Tug-of-War Model with Loose Mechanical Coupling |
title_full |
Transport Properties of Melanosomes along Microtubules Interpreted by a Tug-of-War Model with Loose Mechanical Coupling |
title_fullStr |
Transport Properties of Melanosomes along Microtubules Interpreted by a Tug-of-War Model with Loose Mechanical Coupling |
title_full_unstemmed |
Transport Properties of Melanosomes along Microtubules Interpreted by a Tug-of-War Model with Loose Mechanical Coupling |
title_sort |
transport properties of melanosomes along microtubules interpreted by a tug-of-war model with loose mechanical coupling |
publishDate |
2012 |
url |
http://hdl.handle.net/20.500.12110/paper_19326203_v7_n8_p_Bouzat |
work_keys_str_mv |
AT bouzats transportpropertiesofmelanosomesalongmicrotubulesinterpretedbyatugofwarmodelwithloosemechanicalcoupling AT leviv transportpropertiesofmelanosomesalongmicrotubulesinterpretedbyatugofwarmodelwithloosemechanicalcoupling AT brunol transportpropertiesofmelanosomesalongmicrotubulesinterpretedbyatugofwarmodelwithloosemechanicalcoupling |
_version_ |
1769810251478990848 |