Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression

Mostrar todas las versiones(3)

Dendritic cell (DC) and natural killer (NK) cell interactions are important for the regulation of innate and adaptive immunity, but their relevance during early pregnancy remains elusive. Using two different strategies to manipulate the frequency of NK cells and DC during gestation, we investigated...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	González, I.T., Barrientos, G., Freitag, N., Otto, T., Thijssen, V.L.J.L., Moschansky, P., von Kwiatkowski, P., Klapp, B.F., Winterhager, E., Bauersachs, S., Blois, S.M.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2012
Materias:	angiopoietin 1 glycoprotein p 15095 animal cell animal tissue article cell differentiation cell expansion cell interaction cell proliferation cell shape controlled study decidualization dendritic cell female first trimester pregnancy flow cytometry fluorescence activated cell sorting gene expression histopathology immunogenicity immunohistochemistry inflammation mouse natural killer cell nidation nonhuman pregnancy pregnancy disorder quantitative analysis reverse transcription polymerase chain reaction stroma cell T cell depletion uterine cervix Animals Dendritic Cells Enzyme-Linked Immunosorbent Assay Female Immunohistochemistry Killer Cells, Natural Male Mice Oligonucleotide Array Sequence Analysis Pregnancy Reverse Transcriptase Polymerase Chain Reaction Uterus
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_19326203_v7_n10_p_Gonzalez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_19326203_v7_n10_p_Gonzalez
record_format	dspace
spelling	paperaa:paper_19326203_v7_n10_p_Gonzalez2023-06-12T16:51:32Z Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression PLoS ONE 2012;7(10) González, I.T. Barrientos, G. Freitag, N. Otto, T. Thijssen, V.L.J.L. Moschansky, P. von Kwiatkowski, P. Klapp, B.F. Winterhager, E. Bauersachs, S. Blois, S.M. angiopoietin 1 glycoprotein p 15095 animal cell animal tissue article cell differentiation cell expansion cell interaction cell proliferation cell shape controlled study decidualization dendritic cell female first trimester pregnancy flow cytometry fluorescence activated cell sorting gene expression histopathology immunogenicity immunohistochemistry inflammation mouse natural killer cell nidation nonhuman pregnancy pregnancy disorder quantitative analysis reverse transcription polymerase chain reaction stroma cell T cell depletion uterine cervix Animals Dendritic Cells Enzyme-Linked Immunosorbent Assay Female Immunohistochemistry Killer Cells, Natural Male Mice Oligonucleotide Array Sequence Analysis Pregnancy Reverse Transcriptase Polymerase Chain Reaction Uterus Dendritic cell (DC) and natural killer (NK) cell interactions are important for the regulation of innate and adaptive immunity, but their relevance during early pregnancy remains elusive. Using two different strategies to manipulate the frequency of NK cells and DC during gestation, we investigated their relative impact on the decidualization process and on angiogenic responses that characterize murine implantation. Manipulation of the frequency of NK cells, DC or both lead to a defective decidual response characterized by decreased proliferation and differentiation of stromal cells. Whereas no detrimental effects were evident upon expansion of DC, NK cell ablation in such expanded DC mice severely compromised decidual development and led to early pregnancy loss. Pregnancy failure in these mice was associated with an unbalanced production of anti-angiogenic signals and most notably, with increased expression of genes related to inflammation and immunogenic activation of DC. Thus, NK cells appear to play an important role counteracting potential anomalies raised by DC expansion and overactivity in the decidua, becoming critical for normal pregnancy progression. © 2012 González et al. 2012 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v7_n10_p_Gonzalez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	angiopoietin 1 glycoprotein p 15095 animal cell animal tissue article cell differentiation cell expansion cell interaction cell proliferation cell shape controlled study decidualization dendritic cell female first trimester pregnancy flow cytometry fluorescence activated cell sorting gene expression histopathology immunogenicity immunohistochemistry inflammation mouse natural killer cell nidation nonhuman pregnancy pregnancy disorder quantitative analysis reverse transcription polymerase chain reaction stroma cell T cell depletion uterine cervix Animals Dendritic Cells Enzyme-Linked Immunosorbent Assay Female Immunohistochemistry Killer Cells, Natural Male Mice Oligonucleotide Array Sequence Analysis Pregnancy Reverse Transcriptase Polymerase Chain Reaction Uterus
spellingShingle	angiopoietin 1 glycoprotein p 15095 animal cell animal tissue article cell differentiation cell expansion cell interaction cell proliferation cell shape controlled study decidualization dendritic cell female first trimester pregnancy flow cytometry fluorescence activated cell sorting gene expression histopathology immunogenicity immunohistochemistry inflammation mouse natural killer cell nidation nonhuman pregnancy pregnancy disorder quantitative analysis reverse transcription polymerase chain reaction stroma cell T cell depletion uterine cervix Animals Dendritic Cells Enzyme-Linked Immunosorbent Assay Female Immunohistochemistry Killer Cells, Natural Male Mice Oligonucleotide Array Sequence Analysis Pregnancy Reverse Transcriptase Polymerase Chain Reaction Uterus González, I.T. Barrientos, G. Freitag, N. Otto, T. Thijssen, V.L.J.L. Moschansky, P. von Kwiatkowski, P. Klapp, B.F. Winterhager, E. Bauersachs, S. Blois, S.M. Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression
topic_facet	angiopoietin 1 glycoprotein p 15095 animal cell animal tissue article cell differentiation cell expansion cell interaction cell proliferation cell shape controlled study decidualization dendritic cell female first trimester pregnancy flow cytometry fluorescence activated cell sorting gene expression histopathology immunogenicity immunohistochemistry inflammation mouse natural killer cell nidation nonhuman pregnancy pregnancy disorder quantitative analysis reverse transcription polymerase chain reaction stroma cell T cell depletion uterine cervix Animals Dendritic Cells Enzyme-Linked Immunosorbent Assay Female Immunohistochemistry Killer Cells, Natural Male Mice Oligonucleotide Array Sequence Analysis Pregnancy Reverse Transcriptase Polymerase Chain Reaction Uterus
description	Dendritic cell (DC) and natural killer (NK) cell interactions are important for the regulation of innate and adaptive immunity, but their relevance during early pregnancy remains elusive. Using two different strategies to manipulate the frequency of NK cells and DC during gestation, we investigated their relative impact on the decidualization process and on angiogenic responses that characterize murine implantation. Manipulation of the frequency of NK cells, DC or both lead to a defective decidual response characterized by decreased proliferation and differentiation of stromal cells. Whereas no detrimental effects were evident upon expansion of DC, NK cell ablation in such expanded DC mice severely compromised decidual development and led to early pregnancy loss. Pregnancy failure in these mice was associated with an unbalanced production of anti-angiogenic signals and most notably, with increased expression of genes related to inflammation and immunogenic activation of DC. Thus, NK cells appear to play an important role counteracting potential anomalies raised by DC expansion and overactivity in the decidua, becoming critical for normal pregnancy progression. © 2012 González et al.
format	Artículo Artículo publishedVersion
author	González, I.T. Barrientos, G. Freitag, N. Otto, T. Thijssen, V.L.J.L. Moschansky, P. von Kwiatkowski, P. Klapp, B.F. Winterhager, E. Bauersachs, S. Blois, S.M.
author_facet	González, I.T. Barrientos, G. Freitag, N. Otto, T. Thijssen, V.L.J.L. Moschansky, P. von Kwiatkowski, P. Klapp, B.F. Winterhager, E. Bauersachs, S. Blois, S.M.
author_sort	González, I.T.
title	Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression
title_short	Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression
title_full	Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression
title_fullStr	Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression
title_full_unstemmed	Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression
title_sort	uterine nk cells are critical in shaping dc immunogenic functions compatible with pregnancy progression
publishDate	2012
url	http://hdl.handle.net/20.500.12110/paper_19326203_v7_n10_p_Gonzalez
work_keys_str_mv	AT gonzalezit uterinenkcellsarecriticalinshapingdcimmunogenicfunctionscompatiblewithpregnancyprogression AT barrientosg uterinenkcellsarecriticalinshapingdcimmunogenicfunctionscompatiblewithpregnancyprogression AT freitagn uterinenkcellsarecriticalinshapingdcimmunogenicfunctionscompatiblewithpregnancyprogression AT ottot uterinenkcellsarecriticalinshapingdcimmunogenicfunctionscompatiblewithpregnancyprogression AT thijssenvljl uterinenkcellsarecriticalinshapingdcimmunogenicfunctionscompatiblewithpregnancyprogression AT moschanskyp uterinenkcellsarecriticalinshapingdcimmunogenicfunctionscompatiblewithpregnancyprogression AT vonkwiatkowskip uterinenkcellsarecriticalinshapingdcimmunogenicfunctionscompatiblewithpregnancyprogression AT klappbf uterinenkcellsarecriticalinshapingdcimmunogenicfunctionscompatiblewithpregnancyprogression AT winterhagere uterinenkcellsarecriticalinshapingdcimmunogenicfunctionscompatiblewithpregnancyprogression AT bauersachss uterinenkcellsarecriticalinshapingdcimmunogenicfunctionscompatiblewithpregnancyprogression AT bloissm uterinenkcellsarecriticalinshapingdcimmunogenicfunctionscompatiblewithpregnancyprogression
_version_	1769810146610905088

Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression

Ejemplares similares