New algorithm to determine true colocalization in combination with image restoration and time-lapse confocal microscopy to map Kinases in mitochondria

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The subcellular localization and physiological functions of biomolecules are closely related and thus it is crucial to precisely determine the distribution of different molecules inside the intracellular structures. This is frequently accomplished by fluorescence microscopy with well-characterized m...

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Detalles Bibliográficos
Autores principales: Villalta, J.I., Galli, S., Iacaruso, M.F., Arciuch, V.G.A., Poderoso, J.J., Jares-Erijman, E.A., Pietrasanta, L.I.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2011
Materias:
mitogen activated protein kinase
biological marker
green fluorescent protein
accuracy
algorithm
article
automation
cellular distribution
confocal microscopy
controlled study
fluorescence imaging
human
human cell
image analysis
image reconstruction
mitochondrion
molecular imaging
optical resolution
protein localization
reliability
signal detection
time lapse imaging
animal
cell strain 3T3
fluorescence microscopy
HeLa cell
image processing
metabolism
methodology
mouse
physiology
plasmid
signal transduction
time
Algorithms
Animals
Biological Markers
Green Fluorescent Proteins
HeLa Cells
Humans
Image Processing, Computer-Assisted
MAP Kinase Signaling System
Mice
Microscopy, Confocal
Microscopy, Fluorescence
Mitochondria
NIH 3T3 Cells
Plasmids
Time Factors
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19326203_v6_n4_p_Villalta
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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