Molecular characterization of cryptic and sympatric lymnaeid species from the Galba/Fossaria group in Mendoza Province, Northern Patagonia, Argentina

Background: Freshwater lymnaeid snails can act as the intermediate hosts for trematode parasites such as the liver fluke Fasciola hepatica, that cause significant economic and biomedical burden worldwide, particularly through bovine fascioliasis. Transmission potential is tightly coupled to local co...

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Autores principales: Standley, C.J., Prepelitchi, L., Pietrokovsky, S.M., Issia, L., Stothard, J.R., Wisnivesky-Colli, C.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2013
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_17563305_v6_n1_p_Standley
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spelling paperaa:paper_17563305_v6_n1_p_Standley2023-06-12T16:51:19Z Molecular characterization of cryptic and sympatric lymnaeid species from the Galba/Fossaria group in Mendoza Province, Northern Patagonia, Argentina Parasites Vectors 2013;6(1) Standley, C.J. Prepelitchi, L. Pietrokovsky, S.M. Issia, L. Stothard, J.R. Wisnivesky-Colli, C. Argentina Fascioliasis Freshwater lymnaeid snails Galba Northern Patagonia Population genetics Taxonomy genomic DNA Argentina article DNA extraction Galba Galba truncatula Galba viatrix gene amplification haplotype intermediate host molecular phylogeny morphological trait nonhuman nucleotide sequence parasitology phylogenetic tree taxonomic identification unindexed sequence Bovinae Fasciola Fasciola hepatica Galba truncatula Gastropoda Lymnaeidae Trematoda Viatrix Animals Argentina Cluster Analysis Molecular Sequence Data Phylogeography Sequence Analysis, DNA Snails Background: Freshwater lymnaeid snails can act as the intermediate hosts for trematode parasites such as the liver fluke Fasciola hepatica, that cause significant economic and biomedical burden worldwide, particularly through bovine fascioliasis. Transmission potential is tightly coupled to local compatibility with snail hosts, so accurate identification of lymnaeid species is crucial for understanding disease risk, especially when invasive species are encountered. Mendoza Province, in Argentina, is a center of livestock production and also an area of endemic fascioliasis transmission. However, the distribution of lymnaeid species in the region is not well known. Methods. This study examined lymnaeid snails from seven localities in the Department of Malarguë, Mendoza Province, using morphological and molecular analyses and also describing ecological variables associated with snail presence. Results: While morphological characters identified two species of lymnaeid, Galba truncatula and G. viatrix, molecular data revealed a third, cryptic species, G. neotropica, which was sympatric with G. viatrix. G. truncatula was exclusively found in high altitude (>1900 meters above sea level [masl]) sites, whereas mixed G. neotropica/G. viatrix localities were at middle elevations (1300-1900 masl), and G. viatrix was found alone at the lowest altitude sites (<1300 masl). Phylogenetic analysis using two mitochondrial markers revealed G. neotropica and G. viatrix to be closely related, and given their morphological similarities, their validities as separate taxonomic entities should be questioned. Conclusions: This study highlights the need of a robust taxonomic framework for the identification of lymnaeid snails, incorporating molecular, morphological and ecological variables while avoiding nomenclature redundancy. As the three species observed here, including one alien invasive species, are considered hosts of varying susceptibility to Fasciola parasites, and given the economic importance of fascioliasis for livestock production, this research has critical importance for the ultimate aim of controlling disease transmission. © 2013 Standley et al.; licensee BioMed Central Ltd. Fil:Prepelitchi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pietrokovsky, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wisnivesky-Colli, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_17563305_v6_n1_p_Standley
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic Argentina
Fascioliasis
Freshwater lymnaeid snails
Galba
Northern Patagonia
Population genetics
Taxonomy
genomic DNA
Argentina
article
DNA extraction
Galba
Galba truncatula
Galba viatrix
gene amplification
haplotype
intermediate host
molecular phylogeny
morphological trait
nonhuman
nucleotide sequence
parasitology
phylogenetic tree
taxonomic identification
unindexed sequence
Bovinae
Fasciola
Fasciola hepatica
Galba truncatula
Gastropoda
Lymnaeidae
Trematoda
Viatrix
Animals
Argentina
Cluster Analysis
Molecular Sequence Data
Phylogeography
Sequence Analysis, DNA
Snails
spellingShingle Argentina
Fascioliasis
Freshwater lymnaeid snails
Galba
Northern Patagonia
Population genetics
Taxonomy
genomic DNA
Argentina
article
DNA extraction
Galba
Galba truncatula
Galba viatrix
gene amplification
haplotype
intermediate host
molecular phylogeny
morphological trait
nonhuman
nucleotide sequence
parasitology
phylogenetic tree
taxonomic identification
unindexed sequence
Bovinae
Fasciola
Fasciola hepatica
Galba truncatula
Gastropoda
Lymnaeidae
Trematoda
Viatrix
Animals
Argentina
Cluster Analysis
Molecular Sequence Data
Phylogeography
Sequence Analysis, DNA
Snails
Standley, C.J.
Prepelitchi, L.
Pietrokovsky, S.M.
Issia, L.
Stothard, J.R.
Wisnivesky-Colli, C.
Molecular characterization of cryptic and sympatric lymnaeid species from the Galba/Fossaria group in Mendoza Province, Northern Patagonia, Argentina
topic_facet Argentina
Fascioliasis
Freshwater lymnaeid snails
Galba
Northern Patagonia
Population genetics
Taxonomy
genomic DNA
Argentina
article
DNA extraction
Galba
Galba truncatula
Galba viatrix
gene amplification
haplotype
intermediate host
molecular phylogeny
morphological trait
nonhuman
nucleotide sequence
parasitology
phylogenetic tree
taxonomic identification
unindexed sequence
Bovinae
Fasciola
Fasciola hepatica
Galba truncatula
Gastropoda
Lymnaeidae
Trematoda
Viatrix
Animals
Argentina
Cluster Analysis
Molecular Sequence Data
Phylogeography
Sequence Analysis, DNA
Snails
description Background: Freshwater lymnaeid snails can act as the intermediate hosts for trematode parasites such as the liver fluke Fasciola hepatica, that cause significant economic and biomedical burden worldwide, particularly through bovine fascioliasis. Transmission potential is tightly coupled to local compatibility with snail hosts, so accurate identification of lymnaeid species is crucial for understanding disease risk, especially when invasive species are encountered. Mendoza Province, in Argentina, is a center of livestock production and also an area of endemic fascioliasis transmission. However, the distribution of lymnaeid species in the region is not well known. Methods. This study examined lymnaeid snails from seven localities in the Department of Malarguë, Mendoza Province, using morphological and molecular analyses and also describing ecological variables associated with snail presence. Results: While morphological characters identified two species of lymnaeid, Galba truncatula and G. viatrix, molecular data revealed a third, cryptic species, G. neotropica, which was sympatric with G. viatrix. G. truncatula was exclusively found in high altitude (>1900 meters above sea level [masl]) sites, whereas mixed G. neotropica/G. viatrix localities were at middle elevations (1300-1900 masl), and G. viatrix was found alone at the lowest altitude sites (<1300 masl). Phylogenetic analysis using two mitochondrial markers revealed G. neotropica and G. viatrix to be closely related, and given their morphological similarities, their validities as separate taxonomic entities should be questioned. Conclusions: This study highlights the need of a robust taxonomic framework for the identification of lymnaeid snails, incorporating molecular, morphological and ecological variables while avoiding nomenclature redundancy. As the three species observed here, including one alien invasive species, are considered hosts of varying susceptibility to Fasciola parasites, and given the economic importance of fascioliasis for livestock production, this research has critical importance for the ultimate aim of controlling disease transmission. © 2013 Standley et al.; licensee BioMed Central Ltd.
format Artículo
Artículo
publishedVersion
author Standley, C.J.
Prepelitchi, L.
Pietrokovsky, S.M.
Issia, L.
Stothard, J.R.
Wisnivesky-Colli, C.
author_facet Standley, C.J.
Prepelitchi, L.
Pietrokovsky, S.M.
Issia, L.
Stothard, J.R.
Wisnivesky-Colli, C.
author_sort Standley, C.J.
title Molecular characterization of cryptic and sympatric lymnaeid species from the Galba/Fossaria group in Mendoza Province, Northern Patagonia, Argentina
title_short Molecular characterization of cryptic and sympatric lymnaeid species from the Galba/Fossaria group in Mendoza Province, Northern Patagonia, Argentina
title_full Molecular characterization of cryptic and sympatric lymnaeid species from the Galba/Fossaria group in Mendoza Province, Northern Patagonia, Argentina
title_fullStr Molecular characterization of cryptic and sympatric lymnaeid species from the Galba/Fossaria group in Mendoza Province, Northern Patagonia, Argentina
title_full_unstemmed Molecular characterization of cryptic and sympatric lymnaeid species from the Galba/Fossaria group in Mendoza Province, Northern Patagonia, Argentina
title_sort molecular characterization of cryptic and sympatric lymnaeid species from the galba/fossaria group in mendoza province, northern patagonia, argentina
publishDate 2013
url http://hdl.handle.net/20.500.12110/paper_17563305_v6_n1_p_Standley
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