Differential representation of sunflower ESTs in enriched organ-specific cDNA libraries in a small scale sequencing project

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Background: Subtractive hybridization methods are valuable tools for identifying differentially regulated genes in a given tissue avoiding redundant sequencing of clones representing the same expressed genes, maximizing detection of low abundant transcripts and thus, affecting the efficiency and cos...

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Detalles Bibliográficos
Autores principales:	Fernández, P., Paniego, N., Lew, S., Hopp, H.E., Heinz, R.A.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2003
Materias:	EST Helianthus annuus Organ-specific transcripts SSH Sunflower complementary DNA RNA agronomic trait article breeding controlled study cost effectiveness analysis developmental stage DNA library DNA sequence expressed sequence tag flower gene expression gene function gene identification gene isolation gene sequence genetic association genetic regulation genetic trait genetic transcription marker gene molecular cloning nonhuman nucleotide sequence plant genetics plant leaf plant root plant stem prediction redundancy analysis regulator gene sequence analysis sequence database sequence homology subtractive hybridization sunflower tissue specificity chemistry comparative study gene expression profiling gene expression regulation gene library genetics growth, development and aging methodology nucleic acid hybridization reproducibility sensitivity and specificity Helianthus DNA, Complementary Expressed Sequence Tags Flowers Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Gene Library Nucleic Acid Hybridization Plant Leaves Plant Roots Plant Stems Reproducibility of Results Sensitivity and Specificity Sequence Analysis, DNA
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_14712164_v4_n_p_Fernandez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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