Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice

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A functional interaction between progesterone, Th2 cytokines and a suitable balance between nitric oxide and prostaglandins in the uterus is considered to have a major role in the success of embryo implantation and pregnancy. Non-obese diabetic (NOD) mice offer a suitable model to study the modulato...

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Autores principales:	Roca, V., Larocca, L., Calafat, M., Aisemberg, J., Meiss, R., Franchi, A.M., Leirós, C.P.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2006
Materias:	cytokine gamma interferon interleukin 10 interleukin 12 neuropeptide nitric oxide nitric oxide synthase progesterone prostaglandin prostaglandin synthase tumor necrosis factor alpha vasoactive intestinal polypeptide animal experiment animal model animal tissue article autoimmunity controlled study diabetes mellitus enzyme activity exocrine gland female impaired glucose tolerance mouse nidation nonhuman pregnancy priority journal proestrus protein interaction signal transduction Sjoegren syndrome smooth muscle relaxation Th1 cell Th2 cell uterus vasodilatation Animals Cyclooxygenase Inhibitors Diabetes Mellitus, Type 1 Dinoprostone Enzyme Activation Female Immunohistochemistry Indomethacin Interferon Type II Interleukin-10 Interleukin-12 Mice Mice, Inbred BALB C Mice, Inbred NOD Models, Animal Nitric Oxide Synthase omega-N-Methylarginine Pregnancy Prostaglandin-Endoperoxide Synthases Sjogren's Syndrome Th1 Cells Tumor Necrosis Factor-alpha Uterus Vasoactive Intestinal Peptide
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_14701626_v132_n6_p931_Roca
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paperaa:paper_14701626_v132_n6_p931_Roca2023-06-12T16:50:23Z Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice Reproduction 2006;132(6):931-938 Roca, V. Larocca, L. Calafat, M. Aisemberg, J. Meiss, R. Franchi, A.M. Leirós, C.P. cytokine gamma interferon interleukin 10 interleukin 12 neuropeptide nitric oxide nitric oxide synthase progesterone prostaglandin prostaglandin synthase tumor necrosis factor alpha vasoactive intestinal polypeptide animal experiment animal model animal tissue article autoimmunity controlled study diabetes mellitus enzyme activity exocrine gland female impaired glucose tolerance mouse nidation nonhuman pregnancy priority journal proestrus protein interaction signal transduction Sjoegren syndrome smooth muscle relaxation Th1 cell Th2 cell uterus vasodilatation Animals Cyclooxygenase Inhibitors Diabetes Mellitus, Type 1 Dinoprostone Enzyme Activation Female Immunohistochemistry Indomethacin Interferon Type II Interleukin-10 Interleukin-12 Mice Mice, Inbred BALB C Mice, Inbred NOD Models, Animal Nitric Oxide Synthase omega-N-Methylarginine Pregnancy Prostaglandin-Endoperoxide Synthases Sjogren's Syndrome Th1 Cells Tumor Necrosis Factor-alpha Uterus Vasoactive Intestinal Peptide A functional interaction between progesterone, Th2 cytokines and a suitable balance between nitric oxide and prostaglandins in the uterus is considered to have a major role in the success of embryo implantation and pregnancy. Non-obese diabetic (NOD) mice offer a suitable model to study the modulatory role of Th1 cytokines on uterus signalling and function, since at the prediabetic stage they develop a spontaneous Th1 autoimmune response against exocrine glands similar to Sjögren's syndrome. Vasoactive intestinal peptide (VIP) is a vasoactive neuro- and immunopeptide that promotes Th2 profiles and contributes to the smooth muscle relaxation and vasodilation. The aim of the present study was to investigate the activities of nitric oxide synthase and cyclo-oxygenase and the effect of VIP in the uterus of NOD mice with an emerging Th1 cytokine response. We present evidence of a reduced basal and VIP-stimulated activity of both enzymes in the uterus of NOD mice compared with normal BALB/c mice in proestrus. An altered functional interaction between both enzymes is also present in NOD mice at the time when increased levels of serum interleukin (IL)-12 and tumour necrosis factor-α but not interferon (IFN)-γ or IL-10 were detected. We conclude that signalling alterations in uteri of NOD mice are simultaneous to the onset of a systemic Th1 cytokine response. © 2006 Society for Reproduction and Fertility. Fil:Roca, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Larocca, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calafat, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Aisemberg, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Franchi, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Leirós, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14701626_v132_n6_p931_Roca
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	cytokine gamma interferon interleukin 10 interleukin 12 neuropeptide nitric oxide nitric oxide synthase progesterone prostaglandin prostaglandin synthase tumor necrosis factor alpha vasoactive intestinal polypeptide animal experiment animal model animal tissue article autoimmunity controlled study diabetes mellitus enzyme activity exocrine gland female impaired glucose tolerance mouse nidation nonhuman pregnancy priority journal proestrus protein interaction signal transduction Sjoegren syndrome smooth muscle relaxation Th1 cell Th2 cell uterus vasodilatation Animals Cyclooxygenase Inhibitors Diabetes Mellitus, Type 1 Dinoprostone Enzyme Activation Female Immunohistochemistry Indomethacin Interferon Type II Interleukin-10 Interleukin-12 Mice Mice, Inbred BALB C Mice, Inbred NOD Models, Animal Nitric Oxide Synthase omega-N-Methylarginine Pregnancy Prostaglandin-Endoperoxide Synthases Sjogren's Syndrome Th1 Cells Tumor Necrosis Factor-alpha Uterus Vasoactive Intestinal Peptide
spellingShingle	cytokine gamma interferon interleukin 10 interleukin 12 neuropeptide nitric oxide nitric oxide synthase progesterone prostaglandin prostaglandin synthase tumor necrosis factor alpha vasoactive intestinal polypeptide animal experiment animal model animal tissue article autoimmunity controlled study diabetes mellitus enzyme activity exocrine gland female impaired glucose tolerance mouse nidation nonhuman pregnancy priority journal proestrus protein interaction signal transduction Sjoegren syndrome smooth muscle relaxation Th1 cell Th2 cell uterus vasodilatation Animals Cyclooxygenase Inhibitors Diabetes Mellitus, Type 1 Dinoprostone Enzyme Activation Female Immunohistochemistry Indomethacin Interferon Type II Interleukin-10 Interleukin-12 Mice Mice, Inbred BALB C Mice, Inbred NOD Models, Animal Nitric Oxide Synthase omega-N-Methylarginine Pregnancy Prostaglandin-Endoperoxide Synthases Sjogren's Syndrome Th1 Cells Tumor Necrosis Factor-alpha Uterus Vasoactive Intestinal Peptide Roca, V. Larocca, L. Calafat, M. Aisemberg, J. Meiss, R. Franchi, A.M. Leirós, C.P. Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice
topic_facet	cytokine gamma interferon interleukin 10 interleukin 12 neuropeptide nitric oxide nitric oxide synthase progesterone prostaglandin prostaglandin synthase tumor necrosis factor alpha vasoactive intestinal polypeptide animal experiment animal model animal tissue article autoimmunity controlled study diabetes mellitus enzyme activity exocrine gland female impaired glucose tolerance mouse nidation nonhuman pregnancy priority journal proestrus protein interaction signal transduction Sjoegren syndrome smooth muscle relaxation Th1 cell Th2 cell uterus vasodilatation Animals Cyclooxygenase Inhibitors Diabetes Mellitus, Type 1 Dinoprostone Enzyme Activation Female Immunohistochemistry Indomethacin Interferon Type II Interleukin-10 Interleukin-12 Mice Mice, Inbred BALB C Mice, Inbred NOD Models, Animal Nitric Oxide Synthase omega-N-Methylarginine Pregnancy Prostaglandin-Endoperoxide Synthases Sjogren's Syndrome Th1 Cells Tumor Necrosis Factor-alpha Uterus Vasoactive Intestinal Peptide
description	A functional interaction between progesterone, Th2 cytokines and a suitable balance between nitric oxide and prostaglandins in the uterus is considered to have a major role in the success of embryo implantation and pregnancy. Non-obese diabetic (NOD) mice offer a suitable model to study the modulatory role of Th1 cytokines on uterus signalling and function, since at the prediabetic stage they develop a spontaneous Th1 autoimmune response against exocrine glands similar to Sjögren's syndrome. Vasoactive intestinal peptide (VIP) is a vasoactive neuro- and immunopeptide that promotes Th2 profiles and contributes to the smooth muscle relaxation and vasodilation. The aim of the present study was to investigate the activities of nitric oxide synthase and cyclo-oxygenase and the effect of VIP in the uterus of NOD mice with an emerging Th1 cytokine response. We present evidence of a reduced basal and VIP-stimulated activity of both enzymes in the uterus of NOD mice compared with normal BALB/c mice in proestrus. An altered functional interaction between both enzymes is also present in NOD mice at the time when increased levels of serum interleukin (IL)-12 and tumour necrosis factor-α but not interferon (IFN)-γ or IL-10 were detected. We conclude that signalling alterations in uteri of NOD mice are simultaneous to the onset of a systemic Th1 cytokine response. © 2006 Society for Reproduction and Fertility.
format	Artículo Artículo publishedVersion
author	Roca, V. Larocca, L. Calafat, M. Aisemberg, J. Meiss, R. Franchi, A.M. Leirós, C.P.
author_facet	Roca, V. Larocca, L. Calafat, M. Aisemberg, J. Meiss, R. Franchi, A.M. Leirós, C.P.
author_sort	Roca, V.
title	Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice
title_short	Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice
title_full	Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice
title_fullStr	Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice
title_full_unstemmed	Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice
title_sort	reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice
publishDate	2006
url	http://hdl.handle.net/20.500.12110/paper_14701626_v132_n6_p931_Roca
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_version_	1769810138791673856

Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice

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