Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum

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Aims: To evaluate the antagonistic activity of Fusarium oxysporum nonpathogenic fungal strain S6 against the phytopathogenic fungus Sclerotinia sclerotiorum and to identify the antifungal compounds involved. Methods and Results: The antagonistic activity of Fusarium oxysporum strain S6 was determine...

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Autores principales:	Rodríguez, M.A., Cabrera, G., Godeas, A.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2006
Materias:	Antifungal activity Biocontrol Cyclosporine A Fusarium oxysporum Sclerotinia sclerotiorum Soybean cyclosporin A fungicide fungus antifungal activity article chromatography controlled study correlation analysis dilution evaluation fungal strain fungus culture gene repression greenhouse growth inhibition inoculation metabolite minimum inhibitory concentration nonhuman plant disease purification quantitative assay sclerotinia sclerotiorum soybean spectroscopy survival rate Antifungal Agents Ascomycota Colony Count, Microbial Culture Media Cyclosporine Food Microbiology Fusarium Microbial Sensitivity Tests Pest Control, Biological Soil Microbiology Soybeans Fungi Glycine max
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13645072_v100_n3_p575_Rodriguez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paperaa:paper_13645072_v100_n3_p575_Rodriguez2023-06-12T16:49:54Z Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum J. Appl. Microbiol. 2006;100(3):575-586 Rodríguez, M.A. Cabrera, G. Godeas, A. Antifungal activity Biocontrol Cyclosporine A Fusarium oxysporum Sclerotinia sclerotiorum Soybean cyclosporin A fungicide fungus antifungal activity article chromatography controlled study correlation analysis dilution evaluation fungal strain fungus fungus culture Fusarium oxysporum gene repression greenhouse growth inhibition inoculation metabolite minimum inhibitory concentration nonhuman plant disease purification quantitative assay sclerotinia sclerotiorum soybean spectroscopy survival rate Antifungal Agents Ascomycota Colony Count, Microbial Culture Media Cyclosporine Food Microbiology Fusarium Microbial Sensitivity Tests Pest Control, Biological Soil Microbiology Soybeans Fungi Fusarium oxysporum Glycine max Sclerotinia sclerotiorum Aims: To evaluate the antagonistic activity of Fusarium oxysporum nonpathogenic fungal strain S6 against the phytopathogenic fungus Sclerotinia sclerotiorum and to identify the antifungal compounds involved. Methods and Results: The antagonistic activity of Fusarium oxysporum strain S6 was determined in vitro by dual cultures. The metabolite responsible for the activity was isolated by chromatographic techniques, purified and identified by spectroscopic methods as cyclosporine A. The antifungal activity against the pathogen was correlated with the presence of this metabolite by a dilution assay and then quantified. Cyclosporine A caused both growth inhibition and suppression of sclerotia formation. In a greenhouse assay, a significant increase in the number of surviving soybean (Glycine max) plants was observed when S. sclerotiorum and F. oxysporum (S6) were inoculated together when compared with plants inoculated with S. sclerotiorum alone. Conclusion: Fusarium oxysporum (S6) may be a good fungal biological control agent for S. sclerotiorum and cyclosporine A is the responsible metabolite involved in its antagonistic activity in vitro. Significance and Impact of the Study: Cyclosporine A has not been previously described as an inhibitor of S. sclerotiorum. Its minimum inhibitory concentration (MIC) of 0·1 μg disc-1 makes it suitable to use as a biofungicide. In vivo experiments showed that F. oxysporum (S6) is a good candidate for the biocontrol of S. sclerotiorum in soybean. © 2006 The Society for Applied Microbiology. Fil:Rodríguez, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cabrera, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godeas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13645072_v100_n3_p575_Rodriguez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	Antifungal activity Biocontrol Cyclosporine A Fusarium oxysporum Sclerotinia sclerotiorum Soybean cyclosporin A fungicide fungus antifungal activity article chromatography controlled study correlation analysis dilution evaluation fungal strain fungus fungus culture Fusarium oxysporum gene repression greenhouse growth inhibition inoculation metabolite minimum inhibitory concentration nonhuman plant disease purification quantitative assay sclerotinia sclerotiorum soybean spectroscopy survival rate Antifungal Agents Ascomycota Colony Count, Microbial Culture Media Cyclosporine Food Microbiology Fusarium Microbial Sensitivity Tests Pest Control, Biological Soil Microbiology Soybeans Fungi Fusarium oxysporum Glycine max Sclerotinia sclerotiorum
spellingShingle	Antifungal activity Biocontrol Cyclosporine A Fusarium oxysporum Sclerotinia sclerotiorum Soybean cyclosporin A fungicide fungus antifungal activity article chromatography controlled study correlation analysis dilution evaluation fungal strain fungus fungus culture Fusarium oxysporum gene repression greenhouse growth inhibition inoculation metabolite minimum inhibitory concentration nonhuman plant disease purification quantitative assay sclerotinia sclerotiorum soybean spectroscopy survival rate Antifungal Agents Ascomycota Colony Count, Microbial Culture Media Cyclosporine Food Microbiology Fusarium Microbial Sensitivity Tests Pest Control, Biological Soil Microbiology Soybeans Fungi Fusarium oxysporum Glycine max Sclerotinia sclerotiorum Rodríguez, M.A. Cabrera, G. Godeas, A. Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum
topic_facet	Antifungal activity Biocontrol Cyclosporine A Fusarium oxysporum Sclerotinia sclerotiorum Soybean cyclosporin A fungicide fungus antifungal activity article chromatography controlled study correlation analysis dilution evaluation fungal strain fungus fungus culture Fusarium oxysporum gene repression greenhouse growth inhibition inoculation metabolite minimum inhibitory concentration nonhuman plant disease purification quantitative assay sclerotinia sclerotiorum soybean spectroscopy survival rate Antifungal Agents Ascomycota Colony Count, Microbial Culture Media Cyclosporine Food Microbiology Fusarium Microbial Sensitivity Tests Pest Control, Biological Soil Microbiology Soybeans Fungi Fusarium oxysporum Glycine max Sclerotinia sclerotiorum
description	Aims: To evaluate the antagonistic activity of Fusarium oxysporum nonpathogenic fungal strain S6 against the phytopathogenic fungus Sclerotinia sclerotiorum and to identify the antifungal compounds involved. Methods and Results: The antagonistic activity of Fusarium oxysporum strain S6 was determined in vitro by dual cultures. The metabolite responsible for the activity was isolated by chromatographic techniques, purified and identified by spectroscopic methods as cyclosporine A. The antifungal activity against the pathogen was correlated with the presence of this metabolite by a dilution assay and then quantified. Cyclosporine A caused both growth inhibition and suppression of sclerotia formation. In a greenhouse assay, a significant increase in the number of surviving soybean (Glycine max) plants was observed when S. sclerotiorum and F. oxysporum (S6) were inoculated together when compared with plants inoculated with S. sclerotiorum alone. Conclusion: Fusarium oxysporum (S6) may be a good fungal biological control agent for S. sclerotiorum and cyclosporine A is the responsible metabolite involved in its antagonistic activity in vitro. Significance and Impact of the Study: Cyclosporine A has not been previously described as an inhibitor of S. sclerotiorum. Its minimum inhibitory concentration (MIC) of 0·1 μg disc-1 makes it suitable to use as a biofungicide. In vivo experiments showed that F. oxysporum (S6) is a good candidate for the biocontrol of S. sclerotiorum in soybean. © 2006 The Society for Applied Microbiology.
format	Artículo Artículo publishedVersion
author	Rodríguez, M.A. Cabrera, G. Godeas, A.
author_facet	Rodríguez, M.A. Cabrera, G. Godeas, A.
author_sort	Rodríguez, M.A.
title	Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum
title_short	Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum
title_full	Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum
title_fullStr	Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum
title_full_unstemmed	Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum
title_sort	cyclosporine a from a nonpathogenic fusarium oxysporum suppressing sclerotinia sclerotiorum
publishDate	2006
url	http://hdl.handle.net/20.500.12110/paper_13645072_v100_n3_p575_Rodriguez
work_keys_str_mv	AT rodriguezma cyclosporineafromanonpathogenicfusariumoxysporumsuppressingsclerotiniasclerotiorum AT cabrerag cyclosporineafromanonpathogenicfusariumoxysporumsuppressingsclerotiniasclerotiorum AT godeasa cyclosporineafromanonpathogenicfusariumoxysporumsuppressingsclerotiniasclerotiorum
_version_	1769810133991292928

Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum

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