Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo

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Curcumin (diferuloylmethane) is the active ingredient of the spice plant Curcuma longa and has been shown to act anti-tumorigenic in different types of tumours. Therefore, we have studied its effect in pituitary tumour cell lines and adenomas. Proliferation of lactosomatotroph GH3 and somatotroph Mt...

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Autores principales:	Schaaf, C., Shan, B., Buchfelder, M., Losa, M., Kreutzer, J., Rachinger, W., Stalla, G.K., Schilling, T., Arzt, E., Perone, M.J., Renner, U.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2009
Materias:	7 aminodactinomycin caspase 3 cell cycle protein curcumin cyclin D1 cyclin dependent kinase 4 cyclin dependent kinase inhibitor 1B lipocortin 5 protein bcl 2 antineoplastic activity apoptosis article cancer cell culture cancer inhibition cell cycle arrest cell cycle G2 phase cell cycle M phase cell proliferation cell strain GH3 clinical article colony formation controlled study Curcuma longa DNA fragmentation fluorescence activated cell sorting human human cell hypophysis cell hypophysis tumor immunoblotting in vitro study in vivo study mouse nonhuman nude mouse Animals Antineoplastic Agents Apoptosis Blotting, Western Caspase 3 Cell Cycle Cell Line, Tumor Cell Proliferation Curcumin Cyclin D1 Cyclin-Dependent Kinase 4 Flow Cytometry Humans Male Mice Mice, Nude Pituitary Hormones Pituitary Neoplasms Rats
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13510088_v16_n4_p1339_Schaaf
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paperaa:paper_13510088_v16_n4_p1339_Schaaf2023-06-12T16:49:51Z Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo Endocr.-Relat. Cancer 2009;16(4):1339-1350 Schaaf, C. Shan, B. Buchfelder, M. Losa, M. Kreutzer, J. Rachinger, W. Stalla, G.K. Schilling, T. Arzt, E. Perone, M.J. Renner, U. 7 aminodactinomycin caspase 3 cell cycle protein curcumin cyclin D1 cyclin dependent kinase 4 cyclin dependent kinase inhibitor 1B lipocortin 5 protein bcl 2 antineoplastic activity apoptosis article cancer cell culture cancer inhibition cell cycle arrest cell cycle G2 phase cell cycle M phase cell proliferation cell strain GH3 clinical article colony formation controlled study Curcuma longa DNA fragmentation fluorescence activated cell sorting human human cell hypophysis cell hypophysis tumor immunoblotting in vitro study in vivo study mouse nonhuman nude mouse Animals Antineoplastic Agents Apoptosis Blotting, Western Caspase 3 Cell Cycle Cell Line, Tumor Cell Proliferation Curcumin Cyclin D1 Cyclin-Dependent Kinase 4 Flow Cytometry Humans Male Mice Mice, Nude Pituitary Hormones Pituitary Neoplasms Rats Curcumin (diferuloylmethane) is the active ingredient of the spice plant Curcuma longa and has been shown to act anti-tumorigenic in different types of tumours. Therefore, we have studied its effect in pituitary tumour cell lines and adenomas. Proliferation of lactosomatotroph GH3 and somatotroph MtT/S rat pituitary cells as well as of corticotroph AtT20 mouse pituitary cells was inhibited by curcumin in monolayer cell culture and in colony formation assay in soft agar. Fluorescence-activated cell sorting (FACS) analysis demonstrated curcumin-induced cell cycle arrest at G2/M. Analysis of cell cycle proteins by immunoblotting showed reduction in cyclin D1, cyclin-dependent kinase 4 and no change in p27kip. FACS analysis with Annexin V-FITC/7-aminoactinomycin D staining demonstrated curcumin-induced early apoptosis after 3, 6, 12 and 24 h treatment and nearly no necrosis. Induction of DNA fragmentation, reduction of Bcl-2 and enhancement of cleaved caspase-3 further confirmed induction of apoptosis by curcumin. Growth of GH3 tumours in athymic nude mice was suppressed by curcumin in vivo. In endocrine pituitary tumour cell lines, GH, ACTH and prolactin production were inhibited by curcumin. Studies in 25 human pituitary adenoma cell cultures have confirmed the antitumorigenic and hormone-suppressive effects of curcumin. Altogether, the results described in this report suggest this natural compound as a good candidate for therapeutic use on pituitary tumours. © 2009 Society for Endocrinology Printed in Great Britain. 2009 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13510088_v16_n4_p1339_Schaaf
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	7 aminodactinomycin caspase 3 cell cycle protein curcumin cyclin D1 cyclin dependent kinase 4 cyclin dependent kinase inhibitor 1B lipocortin 5 protein bcl 2 antineoplastic activity apoptosis article cancer cell culture cancer inhibition cell cycle arrest cell cycle G2 phase cell cycle M phase cell proliferation cell strain GH3 clinical article colony formation controlled study Curcuma longa DNA fragmentation fluorescence activated cell sorting human human cell hypophysis cell hypophysis tumor immunoblotting in vitro study in vivo study mouse nonhuman nude mouse Animals Antineoplastic Agents Apoptosis Blotting, Western Caspase 3 Cell Cycle Cell Line, Tumor Cell Proliferation Curcumin Cyclin D1 Cyclin-Dependent Kinase 4 Flow Cytometry Humans Male Mice Mice, Nude Pituitary Hormones Pituitary Neoplasms Rats
spellingShingle	7 aminodactinomycin caspase 3 cell cycle protein curcumin cyclin D1 cyclin dependent kinase 4 cyclin dependent kinase inhibitor 1B lipocortin 5 protein bcl 2 antineoplastic activity apoptosis article cancer cell culture cancer inhibition cell cycle arrest cell cycle G2 phase cell cycle M phase cell proliferation cell strain GH3 clinical article colony formation controlled study Curcuma longa DNA fragmentation fluorescence activated cell sorting human human cell hypophysis cell hypophysis tumor immunoblotting in vitro study in vivo study mouse nonhuman nude mouse Animals Antineoplastic Agents Apoptosis Blotting, Western Caspase 3 Cell Cycle Cell Line, Tumor Cell Proliferation Curcumin Cyclin D1 Cyclin-Dependent Kinase 4 Flow Cytometry Humans Male Mice Mice, Nude Pituitary Hormones Pituitary Neoplasms Rats Schaaf, C. Shan, B. Buchfelder, M. Losa, M. Kreutzer, J. Rachinger, W. Stalla, G.K. Schilling, T. Arzt, E. Perone, M.J. Renner, U. Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo
topic_facet	7 aminodactinomycin caspase 3 cell cycle protein curcumin cyclin D1 cyclin dependent kinase 4 cyclin dependent kinase inhibitor 1B lipocortin 5 protein bcl 2 antineoplastic activity apoptosis article cancer cell culture cancer inhibition cell cycle arrest cell cycle G2 phase cell cycle M phase cell proliferation cell strain GH3 clinical article colony formation controlled study Curcuma longa DNA fragmentation fluorescence activated cell sorting human human cell hypophysis cell hypophysis tumor immunoblotting in vitro study in vivo study mouse nonhuman nude mouse Animals Antineoplastic Agents Apoptosis Blotting, Western Caspase 3 Cell Cycle Cell Line, Tumor Cell Proliferation Curcumin Cyclin D1 Cyclin-Dependent Kinase 4 Flow Cytometry Humans Male Mice Mice, Nude Pituitary Hormones Pituitary Neoplasms Rats
description	Curcumin (diferuloylmethane) is the active ingredient of the spice plant Curcuma longa and has been shown to act anti-tumorigenic in different types of tumours. Therefore, we have studied its effect in pituitary tumour cell lines and adenomas. Proliferation of lactosomatotroph GH3 and somatotroph MtT/S rat pituitary cells as well as of corticotroph AtT20 mouse pituitary cells was inhibited by curcumin in monolayer cell culture and in colony formation assay in soft agar. Fluorescence-activated cell sorting (FACS) analysis demonstrated curcumin-induced cell cycle arrest at G2/M. Analysis of cell cycle proteins by immunoblotting showed reduction in cyclin D1, cyclin-dependent kinase 4 and no change in p27kip. FACS analysis with Annexin V-FITC/7-aminoactinomycin D staining demonstrated curcumin-induced early apoptosis after 3, 6, 12 and 24 h treatment and nearly no necrosis. Induction of DNA fragmentation, reduction of Bcl-2 and enhancement of cleaved caspase-3 further confirmed induction of apoptosis by curcumin. Growth of GH3 tumours in athymic nude mice was suppressed by curcumin in vivo. In endocrine pituitary tumour cell lines, GH, ACTH and prolactin production were inhibited by curcumin. Studies in 25 human pituitary adenoma cell cultures have confirmed the antitumorigenic and hormone-suppressive effects of curcumin. Altogether, the results described in this report suggest this natural compound as a good candidate for therapeutic use on pituitary tumours. © 2009 Society for Endocrinology Printed in Great Britain.
format	Artículo Artículo publishedVersion
author	Schaaf, C. Shan, B. Buchfelder, M. Losa, M. Kreutzer, J. Rachinger, W. Stalla, G.K. Schilling, T. Arzt, E. Perone, M.J. Renner, U.
author_facet	Schaaf, C. Shan, B. Buchfelder, M. Losa, M. Kreutzer, J. Rachinger, W. Stalla, G.K. Schilling, T. Arzt, E. Perone, M.J. Renner, U.
author_sort	Schaaf, C.
title	Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo
title_short	Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo
title_full	Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo
title_fullStr	Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo
title_full_unstemmed	Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo
title_sort	curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo
publishDate	2009
url	http://hdl.handle.net/20.500.12110/paper_13510088_v16_n4_p1339_Schaaf
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Curcumin acts as anti-tumorigenic and hormone-suppressive agent in murine and human pituitary tumour cells in vitro and in vivo

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