Suppression of homologous and homeologous recombination by the bacterial MutS2 protein

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In addition to their role in DNA repair, recombination events are associated with processes aimed at providing the genetic variability needed for adaptation and evolution of a population. In bacteria, recombination is involved in the appearance of new variants by allowing the incorporation of exogen...

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Autores principales:	Pinto, A.V., Mathieu, A., Marsin, S., Veaute, X., Ielpi, L., Labigne, A., Radicella, J.P.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2005
Materias:	bacterial protein DNA protein MSH2 article controlled study DNA repair DNA structure enzyme activity genetic variability Helicobacter pylori mismatch repair nonhuman protein purification sequence homology Adenosine Triphosphatases Bacterial Proteins Base Pair Mismatch Binding Sites DNA Repair DNA, Bacterial DNA-Binding Proteins MutS DNA Mismatch-Binding Protein Recombination, Genetic Bacteria (microorganisms) Helicobacter
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10972765_v17_n1_p113_Pinto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paperaa:paper_10972765_v17_n1_p113_Pinto2023-06-12T16:49:40Z Suppression of homologous and homeologous recombination by the bacterial MutS2 protein Mol. Cell 2005;17(1):113-120 Pinto, A.V. Mathieu, A. Marsin, S. Veaute, X. Ielpi, L. Labigne, A. Radicella, J.P. bacterial protein DNA protein MSH2 article controlled study DNA repair DNA structure enzyme activity genetic variability Helicobacter pylori mismatch repair nonhuman protein purification sequence homology Adenosine Triphosphatases Bacterial Proteins Base Pair Mismatch Binding Sites DNA Repair DNA, Bacterial DNA-Binding Proteins Helicobacter pylori MutS DNA Mismatch-Binding Protein Recombination, Genetic Bacteria (microorganisms) Helicobacter Helicobacter pylori In addition to their role in DNA repair, recombination events are associated with processes aimed at providing the genetic variability needed for adaptation and evolution of a population. In bacteria, recombination is involved in the appearance of new variants by allowing the incorporation of exogenous DNA or the reshuffling of endogenous sequences. Here we show that HpMutS2, a protein belonging to the MutS2 family in Helicobacter pylori, is not involved in mismatch repair but inhibits homologous and homeologous recombination. Disruption of HpmutS2 leads to an increased efficiency of exogenous DNA incorporation. HpMutS2 has a selective affinity for DNA structures mimicking recombination intermediates with no specificity for homoduplex DNA or mismatches. The purified protein has an ATPase activity stimulated by the same DNA structures. Finally, we show that HpMutS2 inhibits DNA strand exchange reactions in vitro. Thus, MutS2 proteins are candidates for controlling recombination and therefore genetic diversity in bacteria. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Radicella, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10972765_v17_n1_p113_Pinto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	bacterial protein DNA protein MSH2 article controlled study DNA repair DNA structure enzyme activity genetic variability Helicobacter pylori mismatch repair nonhuman protein purification sequence homology Adenosine Triphosphatases Bacterial Proteins Base Pair Mismatch Binding Sites DNA Repair DNA, Bacterial DNA-Binding Proteins Helicobacter pylori MutS DNA Mismatch-Binding Protein Recombination, Genetic Bacteria (microorganisms) Helicobacter Helicobacter pylori
spellingShingle	bacterial protein DNA protein MSH2 article controlled study DNA repair DNA structure enzyme activity genetic variability Helicobacter pylori mismatch repair nonhuman protein purification sequence homology Adenosine Triphosphatases Bacterial Proteins Base Pair Mismatch Binding Sites DNA Repair DNA, Bacterial DNA-Binding Proteins Helicobacter pylori MutS DNA Mismatch-Binding Protein Recombination, Genetic Bacteria (microorganisms) Helicobacter Helicobacter pylori Pinto, A.V. Mathieu, A. Marsin, S. Veaute, X. Ielpi, L. Labigne, A. Radicella, J.P. Suppression of homologous and homeologous recombination by the bacterial MutS2 protein
topic_facet	bacterial protein DNA protein MSH2 article controlled study DNA repair DNA structure enzyme activity genetic variability Helicobacter pylori mismatch repair nonhuman protein purification sequence homology Adenosine Triphosphatases Bacterial Proteins Base Pair Mismatch Binding Sites DNA Repair DNA, Bacterial DNA-Binding Proteins Helicobacter pylori MutS DNA Mismatch-Binding Protein Recombination, Genetic Bacteria (microorganisms) Helicobacter Helicobacter pylori
description	In addition to their role in DNA repair, recombination events are associated with processes aimed at providing the genetic variability needed for adaptation and evolution of a population. In bacteria, recombination is involved in the appearance of new variants by allowing the incorporation of exogenous DNA or the reshuffling of endogenous sequences. Here we show that HpMutS2, a protein belonging to the MutS2 family in Helicobacter pylori, is not involved in mismatch repair but inhibits homologous and homeologous recombination. Disruption of HpmutS2 leads to an increased efficiency of exogenous DNA incorporation. HpMutS2 has a selective affinity for DNA structures mimicking recombination intermediates with no specificity for homoduplex DNA or mismatches. The purified protein has an ATPase activity stimulated by the same DNA structures. Finally, we show that HpMutS2 inhibits DNA strand exchange reactions in vitro. Thus, MutS2 proteins are candidates for controlling recombination and therefore genetic diversity in bacteria.
format	Artículo Artículo publishedVersion
author	Pinto, A.V. Mathieu, A. Marsin, S. Veaute, X. Ielpi, L. Labigne, A. Radicella, J.P.
author_facet	Pinto, A.V. Mathieu, A. Marsin, S. Veaute, X. Ielpi, L. Labigne, A. Radicella, J.P.
author_sort	Pinto, A.V.
title	Suppression of homologous and homeologous recombination by the bacterial MutS2 protein
title_short	Suppression of homologous and homeologous recombination by the bacterial MutS2 protein
title_full	Suppression of homologous and homeologous recombination by the bacterial MutS2 protein
title_fullStr	Suppression of homologous and homeologous recombination by the bacterial MutS2 protein
title_full_unstemmed	Suppression of homologous and homeologous recombination by the bacterial MutS2 protein
title_sort	suppression of homologous and homeologous recombination by the bacterial muts2 protein
publishDate	2005
url	http://hdl.handle.net/20.500.12110/paper_10972765_v17_n1_p113_Pinto
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_version_	1769810040543248384

Suppression of homologous and homeologous recombination by the bacterial MutS2 protein

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