A slow RNA polymerase II affects alternative splicing in vivo
Changes in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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2003
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10972765_v12_n2_p525_DeLaMata |
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paperaa:paper_10972765_v12_n2_p525_DeLaMata2023-06-12T16:49:40Z A slow RNA polymerase II affects alternative splicing in vivo Mol. Cell 2003;12(2):525-532 De La Mata, M. Alonso, C.R. Kadener, S. Fededa, J.P. Blaustein, M. Pelisch, F. Cramer, P. Bentley, D. Kornblihtt, A.R. elongation factor fibronectin protein subunit RNA RNA polymerase II Adenovirus article cell culture Drosophila embryo cell exon gene genetic transcription genetic transfection Hox gene human in vivo study point mutation protein expression reverse transcription polymerase chain reaction RNA splicing RNA translation Western blotting Adenoviridae Changes in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation of the Drosophila pol II largest subunit confers on the enzyme a lower elongation rate. Here we show that expression of a human equivalent to Drosophila's C4 pol II in human cultured cells affects alternative splicing of the fibronectin EDI exon and adenovirus E1a pre-mRNA. Most importantly, resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4, which demonstrates the transcriptional control of alternative splicing on an endogenous gene. These results provide a direct proof for the elongation control of alternative splicing in vivo. Fil:De La Mata, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alonso, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kadener, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fededa, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pelisch, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cramer, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10972765_v12_n2_p525_DeLaMata |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
elongation factor fibronectin protein subunit RNA RNA polymerase II Adenovirus article cell culture Drosophila embryo cell exon gene genetic transcription genetic transfection Hox gene human in vivo study point mutation protein expression reverse transcription polymerase chain reaction RNA splicing RNA translation Western blotting Adenoviridae |
| spellingShingle |
elongation factor fibronectin protein subunit RNA RNA polymerase II Adenovirus article cell culture Drosophila embryo cell exon gene genetic transcription genetic transfection Hox gene human in vivo study point mutation protein expression reverse transcription polymerase chain reaction RNA splicing RNA translation Western blotting Adenoviridae De La Mata, M. Alonso, C.R. Kadener, S. Fededa, J.P. Blaustein, M. Pelisch, F. Cramer, P. Bentley, D. Kornblihtt, A.R. A slow RNA polymerase II affects alternative splicing in vivo |
| topic_facet |
elongation factor fibronectin protein subunit RNA RNA polymerase II Adenovirus article cell culture Drosophila embryo cell exon gene genetic transcription genetic transfection Hox gene human in vivo study point mutation protein expression reverse transcription polymerase chain reaction RNA splicing RNA translation Western blotting Adenoviridae |
| description |
Changes in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation of the Drosophila pol II largest subunit confers on the enzyme a lower elongation rate. Here we show that expression of a human equivalent to Drosophila's C4 pol II in human cultured cells affects alternative splicing of the fibronectin EDI exon and adenovirus E1a pre-mRNA. Most importantly, resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4, which demonstrates the transcriptional control of alternative splicing on an endogenous gene. These results provide a direct proof for the elongation control of alternative splicing in vivo. |
| format |
Artículo Artículo publishedVersion |
| author |
De La Mata, M. Alonso, C.R. Kadener, S. Fededa, J.P. Blaustein, M. Pelisch, F. Cramer, P. Bentley, D. Kornblihtt, A.R. |
| author_facet |
De La Mata, M. Alonso, C.R. Kadener, S. Fededa, J.P. Blaustein, M. Pelisch, F. Cramer, P. Bentley, D. Kornblihtt, A.R. |
| author_sort |
De La Mata, M. |
| title |
A slow RNA polymerase II affects alternative splicing in vivo |
| title_short |
A slow RNA polymerase II affects alternative splicing in vivo |
| title_full |
A slow RNA polymerase II affects alternative splicing in vivo |
| title_fullStr |
A slow RNA polymerase II affects alternative splicing in vivo |
| title_full_unstemmed |
A slow RNA polymerase II affects alternative splicing in vivo |
| title_sort |
slow rna polymerase ii affects alternative splicing in vivo |
| publishDate |
2003 |
| url |
http://hdl.handle.net/20.500.12110/paper_10972765_v12_n2_p525_DeLaMata |
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