TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana

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In plant sexual reproduction, water and solute movement are tightly regulated, suggesting the involvement of aquaporins. We previously identified TIP5;1 and TIP1;3 as the only Arabidopsis aquaporin genes that are selectively and highly expressed in mature pollen, and showed that they can transport b...

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Autores principales:	Soto, G., Fox, R., Ayub, N., Alleva, K., Guaimas, F., Erijman, E.J., Mazzella, A., Amodeo, G., Muschietti, J.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2010
Materias:	aquaporins mitochondria nitrogen metabolism pH sensing urea Aquaporins Arabidopsis Arabidopsis thaliana Double mutants In-vitro Plant sexual reproduction Pollen tube Remobilization Single mutant Solute movement Water transport Xenopus oocyte Amino acids Glycoproteins Metabolism Mitochondria pH sensors Urea Nitrogen aquaporin Arabidopsis protein nitrogen plant RNA TIP5;1 protein, Arabidopsis water article gene expression regulation genetics metabolism mitochondrion pH phylogeny pollen tube Arabidopsis Proteins Hydrogen-Ion Concentration Mutagenesis, Insertional Phylogeny Pollen Tube RNA, Plant Water
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09607412_v64_n6_p1038_Soto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_09607412_v64_n6_p1038_Soto
record_format	dspace
spelling	paperaa:paper_09607412_v64_n6_p1038_Soto2023-06-12T16:48:45Z TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana Plant J. 2010;64(6):1038-1047 Soto, G. Fox, R. Ayub, N. Alleva, K. Guaimas, F. Erijman, E.J. Mazzella, A. Amodeo, G. Muschietti, J. aquaporins mitochondria nitrogen metabolism pH sensing urea Aquaporins Arabidopsis Arabidopsis thaliana Double mutants In-vitro nitrogen metabolism pH sensing Plant sexual reproduction Pollen tube Remobilization Single mutant Solute movement Water transport Xenopus oocyte Amino acids Glycoproteins Metabolism Mitochondria pH sensors Urea Nitrogen aquaporin Arabidopsis protein nitrogen plant RNA TIP5;1 protein, Arabidopsis water Arabidopsis article gene expression regulation genetics metabolism mitochondrion pH phylogeny pollen tube Aquaporins Arabidopsis Arabidopsis Proteins Hydrogen-Ion Concentration Mitochondria Mutagenesis, Insertional Nitrogen Phylogeny Pollen Tube RNA, Plant Water Arabidopsis Arabidopsis thaliana In plant sexual reproduction, water and solute movement are tightly regulated, suggesting the involvement of aquaporins. We previously identified TIP5;1 and TIP1;3 as the only Arabidopsis aquaporin genes that are selectively and highly expressed in mature pollen, and showed that they can transport both water and urea when expressed in Xenopus oocytes. Here, we show that TIP5;1 has unusual characteristics, as its water transport activity is regulated by pH. Analysis of the water transport activity of a mutant version of TIP5;1 (TIP5;1-H131A) and amino acid alignment with other plant aquaporins regulated by pH suggested that a conserved motif is involved in pH sensing. GFP-TIP5;1 is located in the mitochondria of pollen tubes. The single mutants tip1;3 and tip5;1, as well as the tip1;3 tip5;1 double mutant, are fertile, but all mutants had shorter than normal pollen tubes when germinated in vitro in the absence of exogenous nitrogen. Thus, we propose that TIP5;1 and TIP1;3 are involved in nitrogen recycling in pollen tubes of Arabidopsis thaliana. © 2010 The Authors. The Plant Journal © 2010 Blackwell Publishing Ltd. Fil:Soto, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fox, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ayub, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mazzella, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muschietti, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09607412_v64_n6_p1038_Soto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	aquaporins mitochondria nitrogen metabolism pH sensing urea Aquaporins Arabidopsis Arabidopsis thaliana Double mutants In-vitro nitrogen metabolism pH sensing Plant sexual reproduction Pollen tube Remobilization Single mutant Solute movement Water transport Xenopus oocyte Amino acids Glycoproteins Metabolism Mitochondria pH sensors Urea Nitrogen aquaporin Arabidopsis protein nitrogen plant RNA TIP5;1 protein, Arabidopsis water Arabidopsis article gene expression regulation genetics metabolism mitochondrion pH phylogeny pollen tube Aquaporins Arabidopsis Arabidopsis Proteins Hydrogen-Ion Concentration Mitochondria Mutagenesis, Insertional Nitrogen Phylogeny Pollen Tube RNA, Plant Water Arabidopsis Arabidopsis thaliana
spellingShingle	aquaporins mitochondria nitrogen metabolism pH sensing urea Aquaporins Arabidopsis Arabidopsis thaliana Double mutants In-vitro nitrogen metabolism pH sensing Plant sexual reproduction Pollen tube Remobilization Single mutant Solute movement Water transport Xenopus oocyte Amino acids Glycoproteins Metabolism Mitochondria pH sensors Urea Nitrogen aquaporin Arabidopsis protein nitrogen plant RNA TIP5;1 protein, Arabidopsis water Arabidopsis article gene expression regulation genetics metabolism mitochondrion pH phylogeny pollen tube Aquaporins Arabidopsis Arabidopsis Proteins Hydrogen-Ion Concentration Mitochondria Mutagenesis, Insertional Nitrogen Phylogeny Pollen Tube RNA, Plant Water Arabidopsis Arabidopsis thaliana Soto, G. Fox, R. Ayub, N. Alleva, K. Guaimas, F. Erijman, E.J. Mazzella, A. Amodeo, G. Muschietti, J. TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana
topic_facet	aquaporins mitochondria nitrogen metabolism pH sensing urea Aquaporins Arabidopsis Arabidopsis thaliana Double mutants In-vitro nitrogen metabolism pH sensing Plant sexual reproduction Pollen tube Remobilization Single mutant Solute movement Water transport Xenopus oocyte Amino acids Glycoproteins Metabolism Mitochondria pH sensors Urea Nitrogen aquaporin Arabidopsis protein nitrogen plant RNA TIP5;1 protein, Arabidopsis water Arabidopsis article gene expression regulation genetics metabolism mitochondrion pH phylogeny pollen tube Aquaporins Arabidopsis Arabidopsis Proteins Hydrogen-Ion Concentration Mitochondria Mutagenesis, Insertional Nitrogen Phylogeny Pollen Tube RNA, Plant Water Arabidopsis Arabidopsis thaliana
description	In plant sexual reproduction, water and solute movement are tightly regulated, suggesting the involvement of aquaporins. We previously identified TIP5;1 and TIP1;3 as the only Arabidopsis aquaporin genes that are selectively and highly expressed in mature pollen, and showed that they can transport both water and urea when expressed in Xenopus oocytes. Here, we show that TIP5;1 has unusual characteristics, as its water transport activity is regulated by pH. Analysis of the water transport activity of a mutant version of TIP5;1 (TIP5;1-H131A) and amino acid alignment with other plant aquaporins regulated by pH suggested that a conserved motif is involved in pH sensing. GFP-TIP5;1 is located in the mitochondria of pollen tubes. The single mutants tip1;3 and tip5;1, as well as the tip1;3 tip5;1 double mutant, are fertile, but all mutants had shorter than normal pollen tubes when germinated in vitro in the absence of exogenous nitrogen. Thus, we propose that TIP5;1 and TIP1;3 are involved in nitrogen recycling in pollen tubes of Arabidopsis thaliana. © 2010 The Authors. The Plant Journal © 2010 Blackwell Publishing Ltd.
format	Artículo Artículo publishedVersion
author	Soto, G. Fox, R. Ayub, N. Alleva, K. Guaimas, F. Erijman, E.J. Mazzella, A. Amodeo, G. Muschietti, J.
author_facet	Soto, G. Fox, R. Ayub, N. Alleva, K. Guaimas, F. Erijman, E.J. Mazzella, A. Amodeo, G. Muschietti, J.
author_sort	Soto, G.
title	TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana
title_short	TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana
title_full	TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana
title_fullStr	TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana
title_full_unstemmed	TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana
title_sort	tip5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in arabidopsis thaliana
publishDate	2010
url	http://hdl.handle.net/20.500.12110/paper_09607412_v64_n6_p1038_Soto
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_version_	1769810237885251584

TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana

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