Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii

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Nucleotide variation was studied in a 1.1 kb section of the coding region of an Esterase gene (Est-A) that maps in the center of the segments rearranged by polymorphic inversions in the cactophilic Drosophila buzzatii. We examine 30 homozygous second-chromosome lines differing in gene arrangement an...

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Autores principales:	Gómez, G.A., Hasson, E.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2003
Materias:	Drosophila buzzatii Esterase-A Gene flow Inversion polymorphism Natural selection Nucleotide variation esterase amino acid substitution animal experiment article DNA polymorphism Drosophila drosophila buzzatii female gene conversion gene frequency gene mapping gene rearrangement genetic code genetic linkage genetic variability nonhuman nucleotide sequence polymorphic locus species differentiation theory Animals Base Sequence DNA DNA Primers Gene Rearrangement In Situ Hybridization Linkage (Genetics) Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Recombination, Genetic Sequence Alignment Sequence Homology, Nucleic Acid Variation (Genetics) Animalia Arachnida Drosophila koepferae Hexapoda
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_07374038_v20_n3_p410_Gomez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_07374038_v20_n3_p410_Gomez
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spelling	paperaa:paper_07374038_v20_n3_p410_Gomez2023-06-12T16:48:08Z Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii Mol. Biol. Evol. 2003;20(3):410-423 Gómez, G.A. Hasson, E. Drosophila buzzatii Esterase-A Gene flow Inversion polymorphism Natural selection Nucleotide variation esterase amino acid substitution animal experiment article DNA polymorphism Drosophila drosophila buzzatii female gene conversion gene frequency gene mapping gene rearrangement genetic code genetic linkage genetic variability nonhuman nucleotide sequence polymorphic locus species differentiation theory Animals Base Sequence DNA DNA Primers Drosophila Gene Rearrangement In Situ Hybridization Linkage (Genetics) Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Recombination, Genetic Sequence Alignment Sequence Homology, Nucleic Acid Variation (Genetics) Animalia Arachnida Drosophila buzzatii Drosophila koepferae Hexapoda Nucleotide variation was studied in a 1.1 kb section of the coding region of an Esterase gene (Est-A) that maps in the center of the segments rearranged by polymorphic inversions in the cactophilic Drosophila buzzatii. We examine 30 homozygous second-chromosome lines differing in gene arrangement and three D. koepferae isofemale lines as outgroups. Our data show that Est-A is a highly polymorphic gene at both synonymous and replacement sites. Significant departures from homogeneity in the distribution of the ratio of silent polymorphism to divergence predicted by the neutral theory reveals a local excess of silent polymorphism. This is consistent with the presence of two apparent narrow peaks of elevated silent polymorphism surrounding nonconservative amino acid substitutions. These polymorphisms as well as others at synonymous and nonsynonymous sites are shared with D. koepferae. We suggest that the presence of shared nucleotide polymorphisms is probably due to interspecific gene flow and/or balancing selection acting on replacement variants and/or to a decreased probability of loss of ancestral polymorphisms caused by linkage to an adaptive inversion polymorphism. Recurrent mutation and persistence of neutral ancestral polymorphisms cannot, however, be ruled out. The analysis of the distribution of nucleotide variation among the three chromosomal arrangements sampled reveals that derived arrangements (J and JZ3) are less polymorphic than the ancestral ST, and that the widely distributed ST and J arrangements are genetically differentiated. However, a significant number of polymorphisms are shared between arrangements, suggesting frequent exchange either from gene conversion or from double crossovers in heterokaryotypes. Finally, our present results in combination with data of sequence variation at the breakpoints of inversion J suggest that this old gene arrangement has risen in frequency in relatively recent times. Fil:Hasson, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07374038_v20_n3_p410_Gomez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	Drosophila buzzatii Esterase-A Gene flow Inversion polymorphism Natural selection Nucleotide variation esterase amino acid substitution animal experiment article DNA polymorphism Drosophila drosophila buzzatii female gene conversion gene frequency gene mapping gene rearrangement genetic code genetic linkage genetic variability nonhuman nucleotide sequence polymorphic locus species differentiation theory Animals Base Sequence DNA DNA Primers Drosophila Gene Rearrangement In Situ Hybridization Linkage (Genetics) Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Recombination, Genetic Sequence Alignment Sequence Homology, Nucleic Acid Variation (Genetics) Animalia Arachnida Drosophila buzzatii Drosophila koepferae Hexapoda
spellingShingle	Drosophila buzzatii Esterase-A Gene flow Inversion polymorphism Natural selection Nucleotide variation esterase amino acid substitution animal experiment article DNA polymorphism Drosophila drosophila buzzatii female gene conversion gene frequency gene mapping gene rearrangement genetic code genetic linkage genetic variability nonhuman nucleotide sequence polymorphic locus species differentiation theory Animals Base Sequence DNA DNA Primers Drosophila Gene Rearrangement In Situ Hybridization Linkage (Genetics) Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Recombination, Genetic Sequence Alignment Sequence Homology, Nucleic Acid Variation (Genetics) Animalia Arachnida Drosophila buzzatii Drosophila koepferae Hexapoda Gómez, G.A. Hasson, E. Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
topic_facet	Drosophila buzzatii Esterase-A Gene flow Inversion polymorphism Natural selection Nucleotide variation esterase amino acid substitution animal experiment article DNA polymorphism Drosophila drosophila buzzatii female gene conversion gene frequency gene mapping gene rearrangement genetic code genetic linkage genetic variability nonhuman nucleotide sequence polymorphic locus species differentiation theory Animals Base Sequence DNA DNA Primers Drosophila Gene Rearrangement In Situ Hybridization Linkage (Genetics) Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Recombination, Genetic Sequence Alignment Sequence Homology, Nucleic Acid Variation (Genetics) Animalia Arachnida Drosophila buzzatii Drosophila koepferae Hexapoda
description	Nucleotide variation was studied in a 1.1 kb section of the coding region of an Esterase gene (Est-A) that maps in the center of the segments rearranged by polymorphic inversions in the cactophilic Drosophila buzzatii. We examine 30 homozygous second-chromosome lines differing in gene arrangement and three D. koepferae isofemale lines as outgroups. Our data show that Est-A is a highly polymorphic gene at both synonymous and replacement sites. Significant departures from homogeneity in the distribution of the ratio of silent polymorphism to divergence predicted by the neutral theory reveals a local excess of silent polymorphism. This is consistent with the presence of two apparent narrow peaks of elevated silent polymorphism surrounding nonconservative amino acid substitutions. These polymorphisms as well as others at synonymous and nonsynonymous sites are shared with D. koepferae. We suggest that the presence of shared nucleotide polymorphisms is probably due to interspecific gene flow and/or balancing selection acting on replacement variants and/or to a decreased probability of loss of ancestral polymorphisms caused by linkage to an adaptive inversion polymorphism. Recurrent mutation and persistence of neutral ancestral polymorphisms cannot, however, be ruled out. The analysis of the distribution of nucleotide variation among the three chromosomal arrangements sampled reveals that derived arrangements (J and JZ3) are less polymorphic than the ancestral ST, and that the widely distributed ST and J arrangements are genetically differentiated. However, a significant number of polymorphisms are shared between arrangements, suggesting frequent exchange either from gene conversion or from double crossovers in heterokaryotypes. Finally, our present results in combination with data of sequence variation at the breakpoints of inversion J suggest that this old gene arrangement has risen in frequency in relatively recent times.
format	Artículo Artículo publishedVersion
author	Gómez, G.A. Hasson, E.
author_facet	Gómez, G.A. Hasson, E.
author_sort	Gómez, G.A.
title	Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
title_short	Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
title_full	Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
title_fullStr	Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
title_full_unstemmed	Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
title_sort	transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii
publishDate	2003
url	http://hdl.handle.net/20.500.12110/paper_07374038_v20_n3_p410_Gomez
work_keys_str_mv	AT gomezga transpecificpolymorphismsinaninversionlinkedesteraselocusindrosophilabuzzatii AT hassone transpecificpolymorphismsinaninversionlinkedesteraselocusindrosophilabuzzatii
_version_	1769810338035793920

Transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii

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