Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium
Malachite green (MG) is a triphenylmethane dye used as a fungicide but also possesses a high toxicity to mammalian cells. The toxicity of MG to Fomes sclerodermeus and Phanerochaete chrysosporium was assessed. P. chrysosporium was highly sensitive to the dye and it was unable to grow on solid media...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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2004
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03781097_v231_n2_p205_Papinutti |
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paperaa:paper_03781097_v231_n2_p205_Papinutti2023-06-12T16:48:01Z Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium FEMS Microbiol. Lett. 2004;231(2):205-209 Papinutti, V.L. Forchiassin, F. Fomes sclerodermeus Laccase Ligninase Phanerochaete chrysosporium White rot fungus 1 hydroxybenzotriazole benzotriazole derivative dye laccase malachite green unclassified drug article chemical modification concentration response controlled study culture medium cytotoxicity decolorization detoxification enzyme activity Fomes sclerodermeus fungicidal activity fungus fungus growth nonhuman Phanerochaete priority journal reaction analysis reduction supernatant Chrysosporium Fomes Fungi Mammalia Phanerochaete chrysosporium Siproeta stelenes Malachite green (MG) is a triphenylmethane dye used as a fungicide but also possesses a high toxicity to mammalian cells. The toxicity of MG to Fomes sclerodermeus and Phanerochaete chrysosporium was assessed. P. chrysosporium was highly sensitive to the dye and it was unable to grow on solid media containing 64 μM of MG, lower concentrations caused a delay in growth. The radial growth of F. sclerodermeus was not affected at this concentration and up to 128 μM. In liquid media both fungi were more sensitive. F. sclerodermeus not only was able to grow in the presence of high concentrations of MG, but also it was able to decolorize and detoxify the dye. MG treated with supernatants containing high laccase activity in the presence or absence of 1-hydroxybenzotriazole (1-HBT) gave a colorless product (DMG) that was not toxic to P. chrysosporium and other white rot fungi tested. On the basis of the data of maximal absorbance, it is probable that the mechanism involved in the modification of the dye was different if 1-HBT was added to the reaction. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. Fil:Papinutti, V.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781097_v231_n2_p205_Papinutti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
Fomes sclerodermeus Laccase Ligninase Phanerochaete chrysosporium White rot fungus 1 hydroxybenzotriazole benzotriazole derivative dye laccase malachite green unclassified drug article chemical modification concentration response controlled study culture medium cytotoxicity decolorization detoxification enzyme activity Fomes sclerodermeus fungicidal activity fungus fungus growth nonhuman Phanerochaete priority journal reaction analysis reduction supernatant Chrysosporium Fomes Fungi Mammalia Phanerochaete chrysosporium Siproeta stelenes |
| spellingShingle |
Fomes sclerodermeus Laccase Ligninase Phanerochaete chrysosporium White rot fungus 1 hydroxybenzotriazole benzotriazole derivative dye laccase malachite green unclassified drug article chemical modification concentration response controlled study culture medium cytotoxicity decolorization detoxification enzyme activity Fomes sclerodermeus fungicidal activity fungus fungus growth nonhuman Phanerochaete priority journal reaction analysis reduction supernatant Chrysosporium Fomes Fungi Mammalia Phanerochaete chrysosporium Siproeta stelenes Papinutti, V.L. Forchiassin, F. Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium |
| topic_facet |
Fomes sclerodermeus Laccase Ligninase Phanerochaete chrysosporium White rot fungus 1 hydroxybenzotriazole benzotriazole derivative dye laccase malachite green unclassified drug article chemical modification concentration response controlled study culture medium cytotoxicity decolorization detoxification enzyme activity Fomes sclerodermeus fungicidal activity fungus fungus growth nonhuman Phanerochaete priority journal reaction analysis reduction supernatant Chrysosporium Fomes Fungi Mammalia Phanerochaete chrysosporium Siproeta stelenes |
| description |
Malachite green (MG) is a triphenylmethane dye used as a fungicide but also possesses a high toxicity to mammalian cells. The toxicity of MG to Fomes sclerodermeus and Phanerochaete chrysosporium was assessed. P. chrysosporium was highly sensitive to the dye and it was unable to grow on solid media containing 64 μM of MG, lower concentrations caused a delay in growth. The radial growth of F. sclerodermeus was not affected at this concentration and up to 128 μM. In liquid media both fungi were more sensitive. F. sclerodermeus not only was able to grow in the presence of high concentrations of MG, but also it was able to decolorize and detoxify the dye. MG treated with supernatants containing high laccase activity in the presence or absence of 1-hydroxybenzotriazole (1-HBT) gave a colorless product (DMG) that was not toxic to P. chrysosporium and other white rot fungi tested. On the basis of the data of maximal absorbance, it is probable that the mechanism involved in the modification of the dye was different if 1-HBT was added to the reaction. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. |
| format |
Artículo Artículo publishedVersion |
| author |
Papinutti, V.L. Forchiassin, F. |
| author_facet |
Papinutti, V.L. Forchiassin, F. |
| author_sort |
Papinutti, V.L. |
| title |
Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium |
| title_short |
Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium |
| title_full |
Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium |
| title_fullStr |
Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium |
| title_full_unstemmed |
Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium |
| title_sort |
modification of malachite green by fomes sclerodermeus and reduction of toxicity to phanerochaete chrysosporium |
| publishDate |
2004 |
| url |
http://hdl.handle.net/20.500.12110/paper_03781097_v231_n2_p205_Papinutti |
| work_keys_str_mv |
AT papinuttivl modificationofmalachitegreenbyfomessclerodermeusandreductionoftoxicitytophanerochaetechrysosporium AT forchiassinf modificationofmalachitegreenbyfomessclerodermeusandreductionoftoxicitytophanerochaetechrysosporium |
| _version_ |
1769810393049333760 |