Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores
The pathways for putrescine biosynthesis and the effects of polyamine biosynthesis inhibitors on the germination and hyphal development of Gigaspora rosea spores were investigated. Incubation of spores with different radioactive substrates demonstrated that both arginine and ornithine decarboxylase...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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2004
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03781097_v230_n1_p115_Sannazzaro |
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paperaa:paper_03781097_v230_n1_p115_Sannazzaro2023-06-12T16:48:00Z Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores FEMS Microbiol. Lett. 2004;230(1):115-121 Sannazzaro, A.I. Álvarez, C.L. Menéndez, A.B. Pieckenstain, F.L. Albertó, E.O. Ruiz, O.A. Arbuscular mycorrhizal fungus Arginine decarboxylase Cyclohexylamine Ornithine decarboxylase α-Difluoromethylarginine α-Difluoromethylornithine alpha difluoromethylarginine arginine decarboxylase cycloheximide eflornithine enzyme inhibitor ornithine decarboxylase polyamine putrescine radioactive material spermidine spermidine synthase spermine article biosynthesis cell viability controlled study enzyme activity fungus fungus hyphae fungus spore germination Gigaspora rosea microbial pest control nonhuman plant disease polyamine synthesis priority journal quantitative analysis Fungi Gigaspora Gigaspora rosea The pathways for putrescine biosynthesis and the effects of polyamine biosynthesis inhibitors on the germination and hyphal development of Gigaspora rosea spores were investigated. Incubation of spores with different radioactive substrates demonstrated that both arginine and ornithine decarboxylase pathways participate in putrescine biosynthesis in G. rosea. Spermidine and spermine were the most abundant polyamines in this fungus. The putrescine biosynthesis inhibitors α-difluoromethylarginine and α-difluoromethylornithine, as well as the spermidine synthase inhibitor cyclohexylamine, slightly decreased polyamine levels. However, only the latter interfered with spore germination. The consequences of the use of putrescine biosynthesis inhibitors for the control of plant pathogenic fungi on the viability of G. rosea spores in soil are discussed. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. Fil:Menéndez, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pieckenstain, F.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Albertó, E.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781097_v230_n1_p115_Sannazzaro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
Arbuscular mycorrhizal fungus Arginine decarboxylase Cyclohexylamine Ornithine decarboxylase α-Difluoromethylarginine α-Difluoromethylornithine alpha difluoromethylarginine arginine decarboxylase cycloheximide eflornithine enzyme inhibitor ornithine decarboxylase polyamine putrescine radioactive material spermidine spermidine synthase spermine article biosynthesis cell viability controlled study enzyme activity fungus fungus hyphae fungus spore germination Gigaspora rosea microbial pest control nonhuman plant disease polyamine synthesis priority journal quantitative analysis Fungi Gigaspora Gigaspora rosea |
| spellingShingle |
Arbuscular mycorrhizal fungus Arginine decarboxylase Cyclohexylamine Ornithine decarboxylase α-Difluoromethylarginine α-Difluoromethylornithine alpha difluoromethylarginine arginine decarboxylase cycloheximide eflornithine enzyme inhibitor ornithine decarboxylase polyamine putrescine radioactive material spermidine spermidine synthase spermine article biosynthesis cell viability controlled study enzyme activity fungus fungus hyphae fungus spore germination Gigaspora rosea microbial pest control nonhuman plant disease polyamine synthesis priority journal quantitative analysis Fungi Gigaspora Gigaspora rosea Sannazzaro, A.I. Álvarez, C.L. Menéndez, A.B. Pieckenstain, F.L. Albertó, E.O. Ruiz, O.A. Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores |
| topic_facet |
Arbuscular mycorrhizal fungus Arginine decarboxylase Cyclohexylamine Ornithine decarboxylase α-Difluoromethylarginine α-Difluoromethylornithine alpha difluoromethylarginine arginine decarboxylase cycloheximide eflornithine enzyme inhibitor ornithine decarboxylase polyamine putrescine radioactive material spermidine spermidine synthase spermine article biosynthesis cell viability controlled study enzyme activity fungus fungus hyphae fungus spore germination Gigaspora rosea microbial pest control nonhuman plant disease polyamine synthesis priority journal quantitative analysis Fungi Gigaspora Gigaspora rosea |
| description |
The pathways for putrescine biosynthesis and the effects of polyamine biosynthesis inhibitors on the germination and hyphal development of Gigaspora rosea spores were investigated. Incubation of spores with different radioactive substrates demonstrated that both arginine and ornithine decarboxylase pathways participate in putrescine biosynthesis in G. rosea. Spermidine and spermine were the most abundant polyamines in this fungus. The putrescine biosynthesis inhibitors α-difluoromethylarginine and α-difluoromethylornithine, as well as the spermidine synthase inhibitor cyclohexylamine, slightly decreased polyamine levels. However, only the latter interfered with spore germination. The consequences of the use of putrescine biosynthesis inhibitors for the control of plant pathogenic fungi on the viability of G. rosea spores in soil are discussed. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. |
| format |
Artículo Artículo publishedVersion |
| author |
Sannazzaro, A.I. Álvarez, C.L. Menéndez, A.B. Pieckenstain, F.L. Albertó, E.O. Ruiz, O.A. |
| author_facet |
Sannazzaro, A.I. Álvarez, C.L. Menéndez, A.B. Pieckenstain, F.L. Albertó, E.O. Ruiz, O.A. |
| author_sort |
Sannazzaro, A.I. |
| title |
Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores |
| title_short |
Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores |
| title_full |
Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores |
| title_fullStr |
Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores |
| title_full_unstemmed |
Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores |
| title_sort |
ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on gigaspora rosea germinating spores |
| publishDate |
2004 |
| url |
http://hdl.handle.net/20.500.12110/paper_03781097_v230_n1_p115_Sannazzaro |
| work_keys_str_mv |
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1769810121563570176 |