Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p

Mostrar todas las versiones(3)

The first specific precursor of porphyrin biosynthesis is δ-aminolevulinic acid. δ-Aminolevulinic acid enters Saccharomyces cerevisiae cells through the γ-aminobutyric acid specific permease Uga4p. It was described that this permease is inducible by γ-aminobutyric acid and its regulation involves se...

Descripción completa

Detalles Bibliográficos
Autores principales:	Garcia, S.C., Moretti, M.B., Batlle, A.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2000
Materias:	γ-Aminobutyric acid δ-Aminolevulinic acid Transcriptional factor Transport regulation Uga4 permease Yeast 4 aminobutyric acid aminolevulinic acid permease article controlled study gene expression regulation nonhuman priority journal promoter region protein transport Saccharomyces cerevisiae transcription regulation Alanine Aminolevulinic Acid beta-Galactosidase DNA-Binding Proteins Fungal Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Membrane Transport Proteins Organic Anion Transporters Promoter Regions (Genetics) Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03781097_v184_n2_p219_Garcia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_03781097_v184_n2_p219_Garcia
record_format	dspace
spelling	paperaa:paper_03781097_v184_n2_p219_Garcia2023-06-12T16:47:59Z Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p FEMS Microbiol. Lett. 2000;184(2):219-224 Garcia, S.C. Moretti, M.B. Batlle, A. γ-Aminobutyric acid δ-Aminolevulinic acid Transcriptional factor Transport regulation Uga4 permease Yeast 4 aminobutyric acid aminolevulinic acid permease article controlled study gene expression regulation nonhuman priority journal promoter region protein transport Saccharomyces cerevisiae transcription regulation Alanine Aminolevulinic Acid beta-Galactosidase DNA-Binding Proteins Fungal Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Membrane Transport Proteins Organic Anion Transporters Promoter Regions (Genetics) Recombinant Fusion Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae The first specific precursor of porphyrin biosynthesis is δ-aminolevulinic acid. δ-Aminolevulinic acid enters Saccharomyces cerevisiae cells through the γ-aminobutyric acid specific permease Uga4p. It was described that this permease is inducible by γ-aminobutyric acid and its regulation involves several specific and pleiotropic transcriptional factors. However, some studies showed that under certain growth conditions the synthesis of Uga4p was not dependent on the presence of γ-aminobutyric acid. To study the effect of the trans-acting factors Uga43p, Uga3p, Uga35p, Ure2p and Gln3p on the expression of UGA4, we measured γ-aminobutyric acid and δ-aminolevulinic acid uptake in yeast mutant cells, lacking one of these regulatory factors, grown under different conditions. Experiments analyzing the UGA4 promoter using a fusion construction UGA4::lacZ were also carried out. The results show that the constitutive expression of the UGA4 gene found in cells under certain growth conditions depends on the presence of Uga3p and Uga35p. In contrast, Gln3p and Ure2p do not seem to have any effect on this constitutive mechanism. Copyright (C) 2000 Federation of European Microbiological Societies. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2000 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781097_v184_n2_p219_Garcia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	γ-Aminobutyric acid δ-Aminolevulinic acid Transcriptional factor Transport regulation Uga4 permease Yeast 4 aminobutyric acid aminolevulinic acid permease article controlled study gene expression regulation nonhuman priority journal promoter region protein transport Saccharomyces cerevisiae transcription regulation Alanine Aminolevulinic Acid beta-Galactosidase DNA-Binding Proteins Fungal Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Membrane Transport Proteins Organic Anion Transporters Promoter Regions (Genetics) Recombinant Fusion Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae
spellingShingle	γ-Aminobutyric acid δ-Aminolevulinic acid Transcriptional factor Transport regulation Uga4 permease Yeast 4 aminobutyric acid aminolevulinic acid permease article controlled study gene expression regulation nonhuman priority journal promoter region protein transport Saccharomyces cerevisiae transcription regulation Alanine Aminolevulinic Acid beta-Galactosidase DNA-Binding Proteins Fungal Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Membrane Transport Proteins Organic Anion Transporters Promoter Regions (Genetics) Recombinant Fusion Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae Garcia, S.C. Moretti, M.B. Batlle, A. Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p
topic_facet	γ-Aminobutyric acid δ-Aminolevulinic acid Transcriptional factor Transport regulation Uga4 permease Yeast 4 aminobutyric acid aminolevulinic acid permease article controlled study gene expression regulation nonhuman priority journal promoter region protein transport Saccharomyces cerevisiae transcription regulation Alanine Aminolevulinic Acid beta-Galactosidase DNA-Binding Proteins Fungal Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Membrane Transport Proteins Organic Anion Transporters Promoter Regions (Genetics) Recombinant Fusion Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae
description	The first specific precursor of porphyrin biosynthesis is δ-aminolevulinic acid. δ-Aminolevulinic acid enters Saccharomyces cerevisiae cells through the γ-aminobutyric acid specific permease Uga4p. It was described that this permease is inducible by γ-aminobutyric acid and its regulation involves several specific and pleiotropic transcriptional factors. However, some studies showed that under certain growth conditions the synthesis of Uga4p was not dependent on the presence of γ-aminobutyric acid. To study the effect of the trans-acting factors Uga43p, Uga3p, Uga35p, Ure2p and Gln3p on the expression of UGA4, we measured γ-aminobutyric acid and δ-aminolevulinic acid uptake in yeast mutant cells, lacking one of these regulatory factors, grown under different conditions. Experiments analyzing the UGA4 promoter using a fusion construction UGA4::lacZ were also carried out. The results show that the constitutive expression of the UGA4 gene found in cells under certain growth conditions depends on the presence of Uga3p and Uga35p. In contrast, Gln3p and Ure2p do not seem to have any effect on this constitutive mechanism. Copyright (C) 2000 Federation of European Microbiological Societies.
format	Artículo Artículo publishedVersion
author	Garcia, S.C. Moretti, M.B. Batlle, A.
author_facet	Garcia, S.C. Moretti, M.B. Batlle, A.
author_sort	Garcia, S.C.
title	Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p
title_short	Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p
title_full	Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p
title_fullStr	Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p
title_full_unstemmed	Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p
title_sort	constitutive expression of the uga4 gene in saccharomyces cerevisiae depends on two positive-acting proteins, uga3p and uga35p
publishDate	2000
url	http://hdl.handle.net/20.500.12110/paper_03781097_v184_n2_p219_Garcia
work_keys_str_mv	AT garciasc constitutiveexpressionoftheuga4geneinsaccharomycescerevisiaedependsontwopositiveactingproteinsuga3panduga35p AT morettimb constitutiveexpressionoftheuga4geneinsaccharomycescerevisiaedependsontwopositiveactingproteinsuga3panduga35p AT batllea constitutiveexpressionoftheuga4geneinsaccharomycescerevisiaedependsontwopositiveactingproteinsuga3panduga35p
_version_	1769810392848007168

Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p

Ejemplares similares