MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2

Mostrar todas las versiones(3)

Prior to this work, we found that adrenal as well as extra-adrenal factors activate the response of renal l 11β-hydroxysteroid dehydrogenase 2 to stressful situations. These results -showing ways through which the organism hinders the pathological occupation of mineralocorticoid receptors by glucoco...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Zallocchi, M.L., Damasco, M.C., Calvo, J.C., Lantos, C.P., Matkovic', L.B.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2006
Materias:
11β-HSD2
Glucocorticoid
Mineralocorticoid
Serotonin
Stress hormones
11beta hydroxysteroid dehydrogenase
11beta hydroxysteroid dehydrogenase 2
corticosterone
dehydrocorticosterone
glucocorticoid
ketanserin
metitepine
mineralocorticoid receptor
serotonin
unclassified drug
animal cell
article
cell strain
chemical analysis
enzyme activation
enzyme activity
enzyme analysis
enzyme inhibition
enzyme substrate
epithelium cell
high performance liquid chromatography
hormone action
nephron
nonhuman
protein expression
11-beta-Hydroxysteroid Dehydrogenase Type 2
Animals
Cell Line
Corticosterone
Dogs
Enzyme Activation
Nephrons
Paracrine Communication
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03279545_v30_n3_p469_Zallocchi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_03279545_v30_n3_p469_Zallocchi
record_format dspace
spelling paperaa:paper_03279545_v30_n3_p469_Zallocchi2023-06-12T16:47:41Z MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2 Biocell 2006;30(3):469-477 Zallocchi, M.L. Damasco, M.C. Calvo, J.C. Lantos, C.P. Matkovic', L.B. 11β-HSD2 Glucocorticoid Mineralocorticoid Serotonin Stress hormones 11beta hydroxysteroid dehydrogenase 11beta hydroxysteroid dehydrogenase 2 corticosterone dehydrocorticosterone glucocorticoid ketanserin metitepine mineralocorticoid receptor serotonin unclassified drug animal cell article cell strain chemical analysis enzyme activation enzyme activity enzyme analysis enzyme inhibition enzyme substrate epithelium cell high performance liquid chromatography hormone action nephron nonhuman protein expression 11-beta-Hydroxysteroid Dehydrogenase Type 2 Animals Cell Line Corticosterone Dogs Enzyme Activation Nephrons Paracrine Communication Serotonin Prior to this work, we found that adrenal as well as extra-adrenal factors activate the response of renal l 11β-hydroxysteroid dehydrogenase 2 to stressful situations. These results -showing ways through which the organism hinders the pathological occupation of mineralocorticoid receptors by glucocorticoids leading to sodium retention and hypertension- prompted the present study on the nature of the above-mentioned extra-adrenal factors. Serotonin was chosen because of its properties as a widely distributed neurohormone, known to interact with glucocorticoids at many sites, also exhibiting increased levels and effects under stressful situations. We studied serotonin effects on 11β-hydroxysteroid dehydrogenase 2 activity in a cell line derived from distal nephron polarized-epithelium, employing 3H-corticosterone as substrate. The end-product, 3H-11- dehydrocorticosterone was separated from the substrate by HPLC and quantified. Serotonin stimulated 11β-hydroxysteroid dehydrogenase 2 activity only at 2nM and 25pM, the magnitude of the response depending also on substrate concentration. The stimulation was blocked by the specific inhibitors methiothepin and ketanserin. We postulate that the organism partially prevents renal mineralocorticoid receptor occupancy by glucocorticoids, circulating at enhanced levels under stressful situations, through serotonin-mediated catabolic regulation of the 11β-hydroxysteroid dehydrogenase 2 activity. Given many, mostly positive, interactions between both hormones, this might eventually pave the way to studies on a new regulatory axis. Fil:Zallocchi, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matkovic', L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03279545_v30_n3_p469_Zallocchi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic 11β-HSD2
Glucocorticoid
Mineralocorticoid
Serotonin
Stress hormones
11beta hydroxysteroid dehydrogenase
11beta hydroxysteroid dehydrogenase 2
corticosterone
dehydrocorticosterone
glucocorticoid
ketanserin
metitepine
mineralocorticoid receptor
serotonin
unclassified drug
animal cell
article
cell strain
chemical analysis
enzyme activation
enzyme activity
enzyme analysis
enzyme inhibition
enzyme substrate
epithelium cell
high performance liquid chromatography
hormone action
nephron
nonhuman
protein expression
11-beta-Hydroxysteroid Dehydrogenase Type 2
Animals
Cell Line
Corticosterone
Dogs
Enzyme Activation
Nephrons
Paracrine Communication
Serotonin
spellingShingle 11β-HSD2
Glucocorticoid
Mineralocorticoid
Serotonin
Stress hormones
11beta hydroxysteroid dehydrogenase
11beta hydroxysteroid dehydrogenase 2
corticosterone
dehydrocorticosterone
glucocorticoid
ketanserin
metitepine
mineralocorticoid receptor
serotonin
unclassified drug
animal cell
article
cell strain
chemical analysis
enzyme activation
enzyme activity
enzyme analysis
enzyme inhibition
enzyme substrate
epithelium cell
high performance liquid chromatography
hormone action
nephron
nonhuman
protein expression
11-beta-Hydroxysteroid Dehydrogenase Type 2
Animals
Cell Line
Corticosterone
Dogs
Enzyme Activation
Nephrons
Paracrine Communication
Serotonin
Zallocchi, M.L.
Damasco, M.C.
Calvo, J.C.
Lantos, C.P.
Matkovic', L.B.
MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2
topic_facet 11β-HSD2
Glucocorticoid
Mineralocorticoid
Serotonin
Stress hormones
11beta hydroxysteroid dehydrogenase
11beta hydroxysteroid dehydrogenase 2
corticosterone
dehydrocorticosterone
glucocorticoid
ketanserin
metitepine
mineralocorticoid receptor
serotonin
unclassified drug
animal cell
article
cell strain
chemical analysis
enzyme activation
enzyme activity
enzyme analysis
enzyme inhibition
enzyme substrate
epithelium cell
high performance liquid chromatography
hormone action
nephron
nonhuman
protein expression
11-beta-Hydroxysteroid Dehydrogenase Type 2
Animals
Cell Line
Corticosterone
Dogs
Enzyme Activation
Nephrons
Paracrine Communication
Serotonin
description Prior to this work, we found that adrenal as well as extra-adrenal factors activate the response of renal l 11β-hydroxysteroid dehydrogenase 2 to stressful situations. These results -showing ways through which the organism hinders the pathological occupation of mineralocorticoid receptors by glucocorticoids leading to sodium retention and hypertension- prompted the present study on the nature of the above-mentioned extra-adrenal factors. Serotonin was chosen because of its properties as a widely distributed neurohormone, known to interact with glucocorticoids at many sites, also exhibiting increased levels and effects under stressful situations. We studied serotonin effects on 11β-hydroxysteroid dehydrogenase 2 activity in a cell line derived from distal nephron polarized-epithelium, employing 3H-corticosterone as substrate. The end-product, 3H-11- dehydrocorticosterone was separated from the substrate by HPLC and quantified. Serotonin stimulated 11β-hydroxysteroid dehydrogenase 2 activity only at 2nM and 25pM, the magnitude of the response depending also on substrate concentration. The stimulation was blocked by the specific inhibitors methiothepin and ketanserin. We postulate that the organism partially prevents renal mineralocorticoid receptor occupancy by glucocorticoids, circulating at enhanced levels under stressful situations, through serotonin-mediated catabolic regulation of the 11β-hydroxysteroid dehydrogenase 2 activity. Given many, mostly positive, interactions between both hormones, this might eventually pave the way to studies on a new regulatory axis.
format Artículo
Artículo
publishedVersion
author Zallocchi, M.L.
Damasco, M.C.
Calvo, J.C.
Lantos, C.P.
Matkovic', L.B.
author_facet Zallocchi, M.L.
Damasco, M.C.
Calvo, J.C.
Lantos, C.P.
Matkovic', L.B.
author_sort Zallocchi, M.L.
title MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2
title_short MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2
title_full MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2
title_fullStr MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2
title_full_unstemmed MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2
title_sort mdck cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2
publishDate 2006
url http://hdl.handle.net/20.500.12110/paper_03279545_v30_n3_p469_Zallocchi
work_keys_str_mv AT zallocchiml mdckcellsexpressserotoninregulable11bhydroxysteroiddehydrogenasetype2
AT damascomc mdckcellsexpressserotoninregulable11bhydroxysteroiddehydrogenasetype2
AT calvojc mdckcellsexpressserotoninregulable11bhydroxysteroiddehydrogenasetype2
AT lantoscp mdckcellsexpressserotoninregulable11bhydroxysteroiddehydrogenasetype2
AT matkoviclb mdckcellsexpressserotoninregulable11bhydroxysteroiddehydrogenasetype2
_version_ 1769810183228227584

Ejemplares similares