Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development

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Endothelial cells, at the cell-cell borders, express PECAM-1, and have been implicated in vascular functions. The monoclonal antibody MEC 13.3 recognizes PECAM-1 molecule from mouse vessels and allows to analyze the ontogeny of mouse endothelium. At the present, little is known about the molecular b...

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Autores principales:	Calabrese, G.C., Wainstok, R.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2004
Materias:	Endothelial cells Mouse organs PECAM-1 Vasculogenesis CD31 antigen glycoprotein monoclonal antibody monoclonal antibody mec 13.3 unclassified drug animal cell antibody detection article blood vessel function brain cell adhesion cell differentiation cell heterogeneity cell population cell suspension embryo endothelium cell enzyme immunoassay fluorescence activated cell sorting intestine liver molecular biology morphological adaptation mouse newborn nonhuman ontogeny phenotype postnatal development prenatal development protein expression Animals Antigens, CD31 Brain Brain Chemistry Cell Adhesion Molecules Cell Differentiation Cells, Cultured Embryo Endothelial Cells Flow Cytometry Immunohistochemistry Intestines Liver Mice Mice, Inbred BALB C Time Factors Animalia Murinae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03279545_v28_n3_p251_Calabrese
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_03279545_v28_n3_p251_Calabrese
record_format	dspace
spelling	paperaa:paper_03279545_v28_n3_p251_Calabrese2023-06-12T16:47:40Z Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development Biocell 2004;28(3):251-258 Calabrese, G.C. Wainstok, R. Endothelial cells Mouse organs PECAM-1 Vasculogenesis CD31 antigen glycoprotein monoclonal antibody monoclonal antibody mec 13.3 unclassified drug animal cell antibody detection article blood vessel function brain cell adhesion cell differentiation cell heterogeneity cell population cell suspension embryo endothelium cell enzyme immunoassay fluorescence activated cell sorting intestine liver molecular biology morphological adaptation mouse newborn nonhuman ontogeny phenotype postnatal development prenatal development protein expression Animals Antigens, CD31 Brain Brain Chemistry Cell Adhesion Molecules Cell Differentiation Cells, Cultured Embryo Endothelial Cells Flow Cytometry Immunohistochemistry Intestines Liver Mice Mice, Inbred BALB C Time Factors Animalia Murinae Endothelial cells, at the cell-cell borders, express PECAM-1, and have been implicated in vascular functions. The monoclonal antibody MEC 13.3 recognizes PECAM-1 molecule from mouse vessels and allows to analyze the ontogeny of mouse endothelium. At the present, little is known about the molecular basis of differentiation pathways of endothelial cells, that enables its morphological heterogeneity. The purpose of this study was to analyze the pattern of PECAM-1 expression, employing monoclonal antibody MEC 13.3, in cellular suspensions obtained from different mouse organs at pre and postnatal stages. Fluorescence activated cell sorter analysis showed a different profile of the glycoprotein expression in a cell population with size and granularity selected by 1G11 endothelial cell line. The expression differs from prenatal to postnatal developmental stages in a given organ, and among the organs studied. Another cell population, with a size and granularity higher than 1G11 endothelial cell line, coexists in cellular suspensions obtained from liver, gut and brain. These cells could be related to those detected by means of immunoenzyme methods which showed a non-differentiated morphology. The different PECAM-1 pattern expression could reflect potential organ-specific differentiation pathways during development and according to organs environment. The existence of another cell population with a size and granularity higher than 1G11 endothelial cell line required a phenotypic characterization. 2004 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03279545_v28_n3_p251_Calabrese
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	Endothelial cells Mouse organs PECAM-1 Vasculogenesis CD31 antigen glycoprotein monoclonal antibody monoclonal antibody mec 13.3 unclassified drug animal cell antibody detection article blood vessel function brain cell adhesion cell differentiation cell heterogeneity cell population cell suspension embryo endothelium cell enzyme immunoassay fluorescence activated cell sorting intestine liver molecular biology morphological adaptation mouse newborn nonhuman ontogeny phenotype postnatal development prenatal development protein expression Animals Antigens, CD31 Brain Brain Chemistry Cell Adhesion Molecules Cell Differentiation Cells, Cultured Embryo Endothelial Cells Flow Cytometry Immunohistochemistry Intestines Liver Mice Mice, Inbred BALB C Time Factors Animalia Murinae
spellingShingle	Endothelial cells Mouse organs PECAM-1 Vasculogenesis CD31 antigen glycoprotein monoclonal antibody monoclonal antibody mec 13.3 unclassified drug animal cell antibody detection article blood vessel function brain cell adhesion cell differentiation cell heterogeneity cell population cell suspension embryo endothelium cell enzyme immunoassay fluorescence activated cell sorting intestine liver molecular biology morphological adaptation mouse newborn nonhuman ontogeny phenotype postnatal development prenatal development protein expression Animals Antigens, CD31 Brain Brain Chemistry Cell Adhesion Molecules Cell Differentiation Cells, Cultured Embryo Endothelial Cells Flow Cytometry Immunohistochemistry Intestines Liver Mice Mice, Inbred BALB C Time Factors Animalia Murinae Calabrese, G.C. Wainstok, R. Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development
topic_facet	Endothelial cells Mouse organs PECAM-1 Vasculogenesis CD31 antigen glycoprotein monoclonal antibody monoclonal antibody mec 13.3 unclassified drug animal cell antibody detection article blood vessel function brain cell adhesion cell differentiation cell heterogeneity cell population cell suspension embryo endothelium cell enzyme immunoassay fluorescence activated cell sorting intestine liver molecular biology morphological adaptation mouse newborn nonhuman ontogeny phenotype postnatal development prenatal development protein expression Animals Antigens, CD31 Brain Brain Chemistry Cell Adhesion Molecules Cell Differentiation Cells, Cultured Embryo Endothelial Cells Flow Cytometry Immunohistochemistry Intestines Liver Mice Mice, Inbred BALB C Time Factors Animalia Murinae
description	Endothelial cells, at the cell-cell borders, express PECAM-1, and have been implicated in vascular functions. The monoclonal antibody MEC 13.3 recognizes PECAM-1 molecule from mouse vessels and allows to analyze the ontogeny of mouse endothelium. At the present, little is known about the molecular basis of differentiation pathways of endothelial cells, that enables its morphological heterogeneity. The purpose of this study was to analyze the pattern of PECAM-1 expression, employing monoclonal antibody MEC 13.3, in cellular suspensions obtained from different mouse organs at pre and postnatal stages. Fluorescence activated cell sorter analysis showed a different profile of the glycoprotein expression in a cell population with size and granularity selected by 1G11 endothelial cell line. The expression differs from prenatal to postnatal developmental stages in a given organ, and among the organs studied. Another cell population, with a size and granularity higher than 1G11 endothelial cell line, coexists in cellular suspensions obtained from liver, gut and brain. These cells could be related to those detected by means of immunoenzyme methods which showed a non-differentiated morphology. The different PECAM-1 pattern expression could reflect potential organ-specific differentiation pathways during development and according to organs environment. The existence of another cell population with a size and granularity higher than 1G11 endothelial cell line required a phenotypic characterization.
format	Artículo Artículo publishedVersion
author	Calabrese, G.C. Wainstok, R.
author_facet	Calabrese, G.C. Wainstok, R.
author_sort	Calabrese, G.C.
title	Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development
title_short	Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development
title_full	Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development
title_fullStr	Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development
title_full_unstemmed	Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development
title_sort	tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development
publishDate	2004
url	http://hdl.handle.net/20.500.12110/paper_03279545_v28_n3_p251_Calabrese
work_keys_str_mv	AT calabresegc tissueexpressionofplateletendothelialcelladhesionmolecule1atpreandpostnatalmurinedevelopment AT wainstokr tissueexpressionofplateletendothelialcelladhesionmolecule1atpreandpostnatalmurinedevelopment
_version_	1769810234130300928

Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development

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