Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms

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The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberis were isolated from 772 quarter milk samples (SCM...

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Detalles Bibliográficos
Autores principales: Lasagno, M.C., Reinoso, E.B., Dieser, S.A., Calvinho, L.F., Buzzola, F., Vissio, C., Bogni, C.I., Odierno, L.M.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2011
Materias:
Bovine subclinical mastitis
Pulsed-field gel electrophoresis
Streptococcus uberis
Virulence factors
cyclic AMP
hyaluronidase
plasminogen activator
Argentina
article
bacterial membrane
bacterial virulence
bacterium isolation
bovine mastitis
dairying
genetic heterogeneity
genotype
milk
nonhuman
phenotype
Animal Husbandry
Animals
Asymptomatic Infections
Bacterial Capsules
Bacterial Proteins
Bacterial Typing Techniques
Cattle
Dairying
DNA, Bacterial
Electrophoresis, Gel, Pulsed-Field
Female
Genotype
Hemolysin Proteins
Hyaluronoglucosaminidase
Mastitis, Bovine
Phenotype
Streptococcal Infections
Streptococcus
Virulence
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03257541_v43_n3_p212_Lasagno
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_03257541_v43_n3_p212_Lasagno
record_format dspace
spelling paperaa:paper_03257541_v43_n3_p212_Lasagno2023-06-12T16:47:38Z Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms Rev. Argent. Microbiol. 2011;43(3):212-217 Lasagno, M.C. Reinoso, E.B. Dieser, S.A. Calvinho, L.F. Buzzola, F. Vissio, C. Bogni, C.I. Odierno, L.M. Bovine subclinical mastitis Pulsed-field gel electrophoresis Streptococcus uberis Virulence factors cyclic AMP hyaluronidase plasminogen activator Argentina article bacterial membrane bacterial virulence bacterium isolation bovine mastitis dairying genetic heterogeneity genotype milk nonhuman phenotype Streptococcus uberis Animal Husbandry Animals Argentina Asymptomatic Infections Bacterial Capsules Bacterial Proteins Bacterial Typing Techniques Cattle Dairying DNA, Bacterial Electrophoresis, Gel, Pulsed-Field Female Genotype Hemolysin Proteins Hyaluronoglucosaminidase Mastitis, Bovine Phenotype Streptococcal Infections Streptococcus Virulence The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberis were isolated from 772 quarter milk samples (SCM > 250,000 cells/ml) collected from 195 cows selected randomly from 18 dairy farms located in Argentina. The S. uberis strains were characterized phenotypically by the presence of virulence factors as plasminogen activator factor (PAF), hyaluronidase (HYA), capsule (CAP) and CAMP factor, and were further characterized genotypically by pulsed-field gel electrophoresis (PFGE). S. uberis strains expressed plasminogen activator factor, hyaluronidase or capsule (65.5 %, 56.3 %, 59.4 %, respectively), but only 25 % of isolates were CAMP factor positive. Thirteen different virulence profiles were identified on the basis of the combination of virulence factors. Eighteen PFGE patterns with 90% of similarity were identified among 32 S. uberis. A great diversity of virulence profiles and PFGE patterns were present among dairy farms. S. uberis strains with the same PFGE pattern showed different virulence profiles. Bovine S. uberis strains causing SCM included in the present study showed heterogeneity in regard to their phenotypic and genotypic characteristics, and the PFGE patterns are not associated with the virulence profiles. Fil:Buzzola, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v43_n3_p212_Lasagno
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic Bovine subclinical mastitis
Pulsed-field gel electrophoresis
Streptococcus uberis
Virulence factors
cyclic AMP
hyaluronidase
plasminogen activator
Argentina
article
bacterial membrane
bacterial virulence
bacterium isolation
bovine mastitis
dairying
genetic heterogeneity
genotype
milk
nonhuman
phenotype
Streptococcus uberis
Animal Husbandry
Animals
Argentina
Asymptomatic Infections
Bacterial Capsules
Bacterial Proteins
Bacterial Typing Techniques
Cattle
Dairying
DNA, Bacterial
Electrophoresis, Gel, Pulsed-Field
Female
Genotype
Hemolysin Proteins
Hyaluronoglucosaminidase
Mastitis, Bovine
Phenotype
Streptococcal Infections
Streptococcus
Virulence
spellingShingle Bovine subclinical mastitis
Pulsed-field gel electrophoresis
Streptococcus uberis
Virulence factors
cyclic AMP
hyaluronidase
plasminogen activator
Argentina
article
bacterial membrane
bacterial virulence
bacterium isolation
bovine mastitis
dairying
genetic heterogeneity
genotype
milk
nonhuman
phenotype
Streptococcus uberis
Animal Husbandry
Animals
Argentina
Asymptomatic Infections
Bacterial Capsules
Bacterial Proteins
Bacterial Typing Techniques
Cattle
Dairying
DNA, Bacterial
Electrophoresis, Gel, Pulsed-Field
Female
Genotype
Hemolysin Proteins
Hyaluronoglucosaminidase
Mastitis, Bovine
Phenotype
Streptococcal Infections
Streptococcus
Virulence
Lasagno, M.C.
Reinoso, E.B.
Dieser, S.A.
Calvinho, L.F.
Buzzola, F.
Vissio, C.
Bogni, C.I.
Odierno, L.M.
Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms
topic_facet Bovine subclinical mastitis
Pulsed-field gel electrophoresis
Streptococcus uberis
Virulence factors
cyclic AMP
hyaluronidase
plasminogen activator
Argentina
article
bacterial membrane
bacterial virulence
bacterium isolation
bovine mastitis
dairying
genetic heterogeneity
genotype
milk
nonhuman
phenotype
Streptococcus uberis
Animal Husbandry
Animals
Argentina
Asymptomatic Infections
Bacterial Capsules
Bacterial Proteins
Bacterial Typing Techniques
Cattle
Dairying
DNA, Bacterial
Electrophoresis, Gel, Pulsed-Field
Female
Genotype
Hemolysin Proteins
Hyaluronoglucosaminidase
Mastitis, Bovine
Phenotype
Streptococcal Infections
Streptococcus
Virulence
description The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberis were isolated from 772 quarter milk samples (SCM > 250,000 cells/ml) collected from 195 cows selected randomly from 18 dairy farms located in Argentina. The S. uberis strains were characterized phenotypically by the presence of virulence factors as plasminogen activator factor (PAF), hyaluronidase (HYA), capsule (CAP) and CAMP factor, and were further characterized genotypically by pulsed-field gel electrophoresis (PFGE). S. uberis strains expressed plasminogen activator factor, hyaluronidase or capsule (65.5 %, 56.3 %, 59.4 %, respectively), but only 25 % of isolates were CAMP factor positive. Thirteen different virulence profiles were identified on the basis of the combination of virulence factors. Eighteen PFGE patterns with 90% of similarity were identified among 32 S. uberis. A great diversity of virulence profiles and PFGE patterns were present among dairy farms. S. uberis strains with the same PFGE pattern showed different virulence profiles. Bovine S. uberis strains causing SCM included in the present study showed heterogeneity in regard to their phenotypic and genotypic characteristics, and the PFGE patterns are not associated with the virulence profiles.
format Artículo
Artículo
publishedVersion
author Lasagno, M.C.
Reinoso, E.B.
Dieser, S.A.
Calvinho, L.F.
Buzzola, F.
Vissio, C.
Bogni, C.I.
Odierno, L.M.
author_facet Lasagno, M.C.
Reinoso, E.B.
Dieser, S.A.
Calvinho, L.F.
Buzzola, F.
Vissio, C.
Bogni, C.I.
Odierno, L.M.
author_sort Lasagno, M.C.
title Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms
title_short Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms
title_full Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms
title_fullStr Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms
title_full_unstemmed Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms
title_sort phenotypic and genotypic characterization of streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms
publishDate 2011
url http://hdl.handle.net/20.500.12110/paper_03257541_v43_n3_p212_Lasagno
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