Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site
Splitting and apparent splicing of ribosomal RNA, both previously unknown in vertebrates, were found in rodents of the genus Ctenomys. Instead of being formed by a single molecule of 4.4 kb, 28S rRNA is split in two molecules of 2.6 and 1.8 kb. A hidden break, mapping within a 106 bp 'intron...
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paperaa:paper_02614189_v18_n11_p3107_Melen2023-06-12T16:47:09Z Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site EMBO J. 1999;18(11):3107-3118 Melen, G.J. Pesce, C.G. Rossi, M.S. Kornblihtt, A.R. Mammalian cells Ribosomal RNA processing RNA 28s RNA precursor animal tissue article fibroblast culture heart intron liver lung male nonhuman priority journal RNA cleavage RNA processing rodent spleen testis tissue specificity Animals Base Sequence Blotting, Northern Cells, Cultured Electrophoresis, Polyacrylamide Gel Evolution, Molecular Introns Male Mice Models, Genetic Molecular Sequence Data Molecular Weight Nucleic Acid Conformation Organ Specificity Rats Repetitive Sequences, Nucleic Acid RNA Precursors RNA Splicing RNA, Ribosomal, 28S Rodentia Testis Thermodynamics Animalia Ctenomys Mammalia Rodentia Vertebrata Splitting and apparent splicing of ribosomal RNA, both previously unknown in vertebrates, were found in rodents of the genus Ctenomys. Instead of being formed by a single molecule of 4.4 kb, 28S rRNA is split in two molecules of 2.6 and 1.8 kb. A hidden break, mapping within a 106 bp 'intron' located in the D6 divergent region, is expressed in mature ribosomes of liver, lung, heart and spleen, as well as in primary fibroblast cultures. Testis-specific processing eliminates the intron and concomitantly the break site, producing non-split 28S rRNA molecules exclusively in this organ. The intron is flanked by two 9 bp direct repeats, revealing the acquisition by insertion of a novel rRNA processing strategy in the evolution of higher organisms. Fil:Melen, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pesce, C.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossi, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1999 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02614189_v18_n11_p3107_Melen |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
language |
Inglés |
orig_language_str_mv |
eng |
topic |
Mammalian cells Ribosomal RNA processing RNA 28s RNA precursor animal tissue article fibroblast culture heart intron liver lung male nonhuman priority journal RNA cleavage RNA processing rodent spleen testis tissue specificity Animals Base Sequence Blotting, Northern Cells, Cultured Electrophoresis, Polyacrylamide Gel Evolution, Molecular Introns Male Mice Models, Genetic Molecular Sequence Data Molecular Weight Nucleic Acid Conformation Organ Specificity Rats Repetitive Sequences, Nucleic Acid RNA Precursors RNA Splicing RNA, Ribosomal, 28S Rodentia Testis Thermodynamics Animalia Ctenomys Mammalia Rodentia Vertebrata |
spellingShingle |
Mammalian cells Ribosomal RNA processing RNA 28s RNA precursor animal tissue article fibroblast culture heart intron liver lung male nonhuman priority journal RNA cleavage RNA processing rodent spleen testis tissue specificity Animals Base Sequence Blotting, Northern Cells, Cultured Electrophoresis, Polyacrylamide Gel Evolution, Molecular Introns Male Mice Models, Genetic Molecular Sequence Data Molecular Weight Nucleic Acid Conformation Organ Specificity Rats Repetitive Sequences, Nucleic Acid RNA Precursors RNA Splicing RNA, Ribosomal, 28S Rodentia Testis Thermodynamics Animalia Ctenomys Mammalia Rodentia Vertebrata Melen, G.J. Pesce, C.G. Rossi, M.S. Kornblihtt, A.R. Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site |
topic_facet |
Mammalian cells Ribosomal RNA processing RNA 28s RNA precursor animal tissue article fibroblast culture heart intron liver lung male nonhuman priority journal RNA cleavage RNA processing rodent spleen testis tissue specificity Animals Base Sequence Blotting, Northern Cells, Cultured Electrophoresis, Polyacrylamide Gel Evolution, Molecular Introns Male Mice Models, Genetic Molecular Sequence Data Molecular Weight Nucleic Acid Conformation Organ Specificity Rats Repetitive Sequences, Nucleic Acid RNA Precursors RNA Splicing RNA, Ribosomal, 28S Rodentia Testis Thermodynamics Animalia Ctenomys Mammalia Rodentia Vertebrata |
description |
Splitting and apparent splicing of ribosomal RNA, both previously unknown in vertebrates, were found in rodents of the genus Ctenomys. Instead of being formed by a single molecule of 4.4 kb, 28S rRNA is split in two molecules of 2.6 and 1.8 kb. A hidden break, mapping within a 106 bp 'intron' located in the D6 divergent region, is expressed in mature ribosomes of liver, lung, heart and spleen, as well as in primary fibroblast cultures. Testis-specific processing eliminates the intron and concomitantly the break site, producing non-split 28S rRNA molecules exclusively in this organ. The intron is flanked by two 9 bp direct repeats, revealing the acquisition by insertion of a novel rRNA processing strategy in the evolution of higher organisms. |
format |
Artículo Artículo publishedVersion |
author |
Melen, G.J. Pesce, C.G. Rossi, M.S. Kornblihtt, A.R. |
author_facet |
Melen, G.J. Pesce, C.G. Rossi, M.S. Kornblihtt, A.R. |
author_sort |
Melen, G.J. |
title |
Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site |
title_short |
Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site |
title_full |
Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site |
title_fullStr |
Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site |
title_full_unstemmed |
Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site |
title_sort |
novel processing in a mammalian nuclear 28s pre-rrna: tissue-specific elimination of an 'intron' bearing a hidden break site |
publishDate |
1999 |
url |
http://hdl.handle.net/20.500.12110/paper_02614189_v18_n11_p3107_Melen |
work_keys_str_mv |
AT melengj novelprocessinginamammaliannuclear28sprerrnatissuespecificeliminationofanintronbearingahiddenbreaksite AT pescecg novelprocessinginamammaliannuclear28sprerrnatissuespecificeliminationofanintronbearingahiddenbreaksite AT rossims novelprocessinginamammaliannuclear28sprerrnatissuespecificeliminationofanintronbearingahiddenbreaksite AT kornblihttar novelprocessinginamammaliannuclear28sprerrnatissuespecificeliminationofanintronbearingahiddenbreaksite |
_version_ |
1769810231361011712 |