A novel procedure to measure the antioxidant capacity of yerba maté extracts
Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult t...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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2012
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01012061_v32_n1_p126_Hartwig |
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paperaa:paper_01012061_v32_n1_p126_Hartwig2023-06-12T16:46:26Z A novel procedure to measure the antioxidant capacity of yerba maté extracts Cienc. Tecnol. Aliment. 2012;32(1):126-133 Hartwig, V.G. Brumovsky, L.A. Fretes, R.M. Boado, L.S. Antioxidant capacity Dpph Ilex paraguariensis Yerba maté Croton ovalifolius Ilex paraguariensis Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult to compare the results between studies because this assay is applied in too many different conditions by the different research groups. Thus, in order to assess the antioxidant capacity of yerba maté extracts, the following procedure is proposed: 100 μL of an aqueous dilution of the extracts is mixed in duplicate with 3.0 mL of a DPPH 'work solution in absolute methanol (100 μM.L-1), with an incubation time of 120 minutes in darkness at 37 ± 1 °C, and then absorbance is read at 517 nm against absolute methanol. The results should be expressed as ascorbic acid equivalents or Trolox equivalents in mass percentage (g% dm, dry matter) in order to facilitate comparisons. The AOC of the ethanolic extracts ranged between 12.8 and 23.1 g TE % dm and from 9.1 to 16.4 g AAE % dm. The AOC determined by the DPPH assay proposed in the present study can be related to the total polyphenolic content determined by the Folin-Ciocalteu assay. 2012 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01012061_v32_n1_p126_Hartwig |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
Antioxidant capacity Dpph Ilex paraguariensis Yerba maté Croton ovalifolius Ilex paraguariensis |
| spellingShingle |
Antioxidant capacity Dpph Ilex paraguariensis Yerba maté Croton ovalifolius Ilex paraguariensis Hartwig, V.G. Brumovsky, L.A. Fretes, R.M. Boado, L.S. A novel procedure to measure the antioxidant capacity of yerba maté extracts |
| topic_facet |
Antioxidant capacity Dpph Ilex paraguariensis Yerba maté Croton ovalifolius Ilex paraguariensis |
| description |
Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult to compare the results between studies because this assay is applied in too many different conditions by the different research groups. Thus, in order to assess the antioxidant capacity of yerba maté extracts, the following procedure is proposed: 100 μL of an aqueous dilution of the extracts is mixed in duplicate with 3.0 mL of a DPPH 'work solution in absolute methanol (100 μM.L-1), with an incubation time of 120 minutes in darkness at 37 ± 1 °C, and then absorbance is read at 517 nm against absolute methanol. The results should be expressed as ascorbic acid equivalents or Trolox equivalents in mass percentage (g% dm, dry matter) in order to facilitate comparisons. The AOC of the ethanolic extracts ranged between 12.8 and 23.1 g TE % dm and from 9.1 to 16.4 g AAE % dm. The AOC determined by the DPPH assay proposed in the present study can be related to the total polyphenolic content determined by the Folin-Ciocalteu assay. |
| format |
Artículo Artículo publishedVersion |
| author |
Hartwig, V.G. Brumovsky, L.A. Fretes, R.M. Boado, L.S. |
| author_facet |
Hartwig, V.G. Brumovsky, L.A. Fretes, R.M. Boado, L.S. |
| author_sort |
Hartwig, V.G. |
| title |
A novel procedure to measure the antioxidant capacity of yerba maté extracts |
| title_short |
A novel procedure to measure the antioxidant capacity of yerba maté extracts |
| title_full |
A novel procedure to measure the antioxidant capacity of yerba maté extracts |
| title_fullStr |
A novel procedure to measure the antioxidant capacity of yerba maté extracts |
| title_full_unstemmed |
A novel procedure to measure the antioxidant capacity of yerba maté extracts |
| title_sort |
novel procedure to measure the antioxidant capacity of yerba maté extracts |
| publishDate |
2012 |
| url |
http://hdl.handle.net/20.500.12110/paper_01012061_v32_n1_p126_Hartwig |
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