The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family

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In the same way that cry genes, coding for larvicidal delta endotoxins, constitute a large and diverse gene family, the cyt genes for hemolytic toxins seem to compose another set of highly related genes in Bacillus thuringiensis. Although the occurrence of Cyt hemolytic factors in B. thuringiensis h...

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Autores principales:	Guerchicoff, A., Delécluse, A., Rubinstein, C.P.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2001
Materias:	bacterial protein bacterial toxin endotoxin hemolysin insecticidal crystal protein, Bacillus Thuringiensis amino acid sequence article Bacillus thuringiensis bacterial gene classification DNA sequence genetics metabolism methodology molecular genetics multigene family polymerase chain reaction sequence alignment Amino Acid Sequence Bacterial Proteins Bacterial Toxins Endotoxins Genes, Bacterial Hemolysin Proteins Molecular Sequence Data Multigene Family Polymerase Chain Reaction Sequence Alignment Sequence Analysis, DNA
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3_p1090_Guerchicoff
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paperaa:paper_00992240_v67_n3_p1090_Guerchicoff2023-06-12T16:46:21Z The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family Appl. Environ. Microbiol. 2001;67(3):1090-1096 Guerchicoff, A. Delécluse, A. Rubinstein, C.P. bacterial protein bacterial toxin endotoxin hemolysin insecticidal crystal protein, Bacillus Thuringiensis amino acid sequence article Bacillus thuringiensis bacterial gene classification DNA sequence genetics metabolism methodology molecular genetics multigene family polymerase chain reaction sequence alignment Amino Acid Sequence Bacillus thuringiensis Bacterial Proteins Bacterial Toxins Endotoxins Genes, Bacterial Hemolysin Proteins Molecular Sequence Data Multigene Family Polymerase Chain Reaction Sequence Alignment Sequence Analysis, DNA In the same way that cry genes, coding for larvicidal delta endotoxins, constitute a large and diverse gene family, the cyt genes for hemolytic toxins seem to compose another set of highly related genes in Bacillus thuringiensis. Although the occurrence of Cyt hemolytic factors in B. thuringiensis has been typically associated with mosquitocidal strains, we have recently shown that cyt genes are also present in strains with different pathotypes; this is the case for the morrisoni subspecies, which includes strains biologically active against dipteran, lepidopteran, and coleopteran larvae. In addition, while one Cyt type of protein has been described in all of the mosquitocidal strains studied so far, the present study confirms that at least two Cyt toxins coexist in the more toxic antidipteran strains, such as B. thuringiensis subsp. israelensis and subsp. morrisoni PG14, and that this could also be the case for many others. In fact, PCR screening and Western blot analysis of 50 B. thuringiensis strains revealed that cyt2-related genes are present in all strains with known antidipteran activity, as well as in some others with different or unknown host ranges. Partial DNA sequences for several of these genes were determined, and protein sequence alignments revealed a high degree of conservation of the structural domains. These findings point to an important biological role for Cyt toxins in the final in vivo toxic activity of many B. thuringiensis strains. Fil:Rubinstein, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3_p1090_Guerchicoff
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	bacterial protein bacterial toxin endotoxin hemolysin insecticidal crystal protein, Bacillus Thuringiensis amino acid sequence article Bacillus thuringiensis bacterial gene classification DNA sequence genetics metabolism methodology molecular genetics multigene family polymerase chain reaction sequence alignment Amino Acid Sequence Bacillus thuringiensis Bacterial Proteins Bacterial Toxins Endotoxins Genes, Bacterial Hemolysin Proteins Molecular Sequence Data Multigene Family Polymerase Chain Reaction Sequence Alignment Sequence Analysis, DNA
spellingShingle	bacterial protein bacterial toxin endotoxin hemolysin insecticidal crystal protein, Bacillus Thuringiensis amino acid sequence article Bacillus thuringiensis bacterial gene classification DNA sequence genetics metabolism methodology molecular genetics multigene family polymerase chain reaction sequence alignment Amino Acid Sequence Bacillus thuringiensis Bacterial Proteins Bacterial Toxins Endotoxins Genes, Bacterial Hemolysin Proteins Molecular Sequence Data Multigene Family Polymerase Chain Reaction Sequence Alignment Sequence Analysis, DNA Guerchicoff, A. Delécluse, A. Rubinstein, C.P. The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family
topic_facet	bacterial protein bacterial toxin endotoxin hemolysin insecticidal crystal protein, Bacillus Thuringiensis amino acid sequence article Bacillus thuringiensis bacterial gene classification DNA sequence genetics metabolism methodology molecular genetics multigene family polymerase chain reaction sequence alignment Amino Acid Sequence Bacillus thuringiensis Bacterial Proteins Bacterial Toxins Endotoxins Genes, Bacterial Hemolysin Proteins Molecular Sequence Data Multigene Family Polymerase Chain Reaction Sequence Alignment Sequence Analysis, DNA
description	In the same way that cry genes, coding for larvicidal delta endotoxins, constitute a large and diverse gene family, the cyt genes for hemolytic toxins seem to compose another set of highly related genes in Bacillus thuringiensis. Although the occurrence of Cyt hemolytic factors in B. thuringiensis has been typically associated with mosquitocidal strains, we have recently shown that cyt genes are also present in strains with different pathotypes; this is the case for the morrisoni subspecies, which includes strains biologically active against dipteran, lepidopteran, and coleopteran larvae. In addition, while one Cyt type of protein has been described in all of the mosquitocidal strains studied so far, the present study confirms that at least two Cyt toxins coexist in the more toxic antidipteran strains, such as B. thuringiensis subsp. israelensis and subsp. morrisoni PG14, and that this could also be the case for many others. In fact, PCR screening and Western blot analysis of 50 B. thuringiensis strains revealed that cyt2-related genes are present in all strains with known antidipteran activity, as well as in some others with different or unknown host ranges. Partial DNA sequences for several of these genes were determined, and protein sequence alignments revealed a high degree of conservation of the structural domains. These findings point to an important biological role for Cyt toxins in the final in vivo toxic activity of many B. thuringiensis strains.
format	Artículo Artículo publishedVersion
author	Guerchicoff, A. Delécluse, A. Rubinstein, C.P.
author_facet	Guerchicoff, A. Delécluse, A. Rubinstein, C.P.
author_sort	Guerchicoff, A.
title	The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family
title_short	The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family
title_full	The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family
title_fullStr	The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family
title_full_unstemmed	The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family
title_sort	bacillus thuringiensis cyt genes for hemolytic endotoxins constitute a gene family
publishDate	2001
url	http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3_p1090_Guerchicoff
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The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family

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