Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs
This study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estera, Argentina, were collected in N...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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1998
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00740276_v93_n4_p501_Lauricella |
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paperaa:paper_00740276_v93_n4_p501_Lauricella2023-06-12T16:45:49Z Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs Mem. Inst. Oswaldo Cruz 1998;93(4):501-507 Lauricella, M.A. Castañera, M.B. Gürtler, R.E. Segura, E.L. Chronic infection Dogs Serology Surveillance Trypanosoma cruzi Canis familiaris Trypanosoma Trypanosoma cruzi protozoon antibody animal animal disease Argentina article blood Chagas disease dog dog disease enzyme linked immunosorbent assay hemagglutination test immunology sensitivity and specificity standard Trypanosoma cruzi Animals Antibodies, Protozoan Argentina Chagas Disease Dog Diseases Dogs Enzyme-Linked Immunosorbent Assay Hemagglutination Tests Sensitivity and Specificity Trypanosoma cruzi This study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estera, Argentina, were collected in November 1994 and preserved in buffered neutral glycerin. All sera were tested by indirect hemagglutination test (IHAT), indirect immunofluorescence test (IFAT), and ELISA using the flagellar fraction of T. cruzi as antigen. Dog sera from an area without vectorial transmission were used to calculate ELISA specificity and cut-off value. Eighty-six percent of sera had concordant results for all tests. All sera reactive for IHAT and IFAT were also reactive for ELISA, except in one case. Sera tested by ELISA when diluted 1 : 200 allowed a clearer division between non-reactive and reactive sera than when 1 : 100 with greater agreement among serologic techniques. The specificity of ELISA was 96.2%. Among 34 adult dogs with a positive xenodiagnosis, sensitivity was 94% both for ELISA and IFAT. ELISA is the first choice for screening purposes and one of the pair of techniques recommended for diagnostic studies in dog populations. Fil:Lauricella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castañera, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00740276_v93_n4_p501_Lauricella |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
Chronic infection Dogs Serology Surveillance Trypanosoma cruzi Canis familiaris Trypanosoma Trypanosoma cruzi protozoon antibody animal animal disease Argentina article blood Chagas disease dog dog disease enzyme linked immunosorbent assay hemagglutination test immunology sensitivity and specificity standard Trypanosoma cruzi Animals Antibodies, Protozoan Argentina Chagas Disease Dog Diseases Dogs Enzyme-Linked Immunosorbent Assay Hemagglutination Tests Sensitivity and Specificity Trypanosoma cruzi |
| spellingShingle |
Chronic infection Dogs Serology Surveillance Trypanosoma cruzi Canis familiaris Trypanosoma Trypanosoma cruzi protozoon antibody animal animal disease Argentina article blood Chagas disease dog dog disease enzyme linked immunosorbent assay hemagglutination test immunology sensitivity and specificity standard Trypanosoma cruzi Animals Antibodies, Protozoan Argentina Chagas Disease Dog Diseases Dogs Enzyme-Linked Immunosorbent Assay Hemagglutination Tests Sensitivity and Specificity Trypanosoma cruzi Lauricella, M.A. Castañera, M.B. Gürtler, R.E. Segura, E.L. Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
| topic_facet |
Chronic infection Dogs Serology Surveillance Trypanosoma cruzi Canis familiaris Trypanosoma Trypanosoma cruzi protozoon antibody animal animal disease Argentina article blood Chagas disease dog dog disease enzyme linked immunosorbent assay hemagglutination test immunology sensitivity and specificity standard Trypanosoma cruzi Animals Antibodies, Protozoan Argentina Chagas Disease Dog Diseases Dogs Enzyme-Linked Immunosorbent Assay Hemagglutination Tests Sensitivity and Specificity Trypanosoma cruzi |
| description |
This study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estera, Argentina, were collected in November 1994 and preserved in buffered neutral glycerin. All sera were tested by indirect hemagglutination test (IHAT), indirect immunofluorescence test (IFAT), and ELISA using the flagellar fraction of T. cruzi as antigen. Dog sera from an area without vectorial transmission were used to calculate ELISA specificity and cut-off value. Eighty-six percent of sera had concordant results for all tests. All sera reactive for IHAT and IFAT were also reactive for ELISA, except in one case. Sera tested by ELISA when diluted 1 : 200 allowed a clearer division between non-reactive and reactive sera than when 1 : 100 with greater agreement among serologic techniques. The specificity of ELISA was 96.2%. Among 34 adult dogs with a positive xenodiagnosis, sensitivity was 94% both for ELISA and IFAT. ELISA is the first choice for screening purposes and one of the pair of techniques recommended for diagnostic studies in dog populations. |
| format |
Artículo Artículo publishedVersion |
| author |
Lauricella, M.A. Castañera, M.B. Gürtler, R.E. Segura, E.L. |
| author_facet |
Lauricella, M.A. Castañera, M.B. Gürtler, R.E. Segura, E.L. |
| author_sort |
Lauricella, M.A. |
| title |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
| title_short |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
| title_full |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
| title_fullStr |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
| title_full_unstemmed |
Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs |
| title_sort |
immunodiagnosis of trypanosoma cruzi (chagas' disease) infection in naturally infected dogs |
| publishDate |
1998 |
| url |
http://hdl.handle.net/20.500.12110/paper_00740276_v93_n4_p501_Lauricella |
| work_keys_str_mv |
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| _version_ |
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