The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener

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By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a sing...

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Autores principales: Santos, M.R.M., Cano, M.I., Schijman, A., Lorenzi, H., Vázquez, M., Levin, M.J., Ramirez, J.L., Brandão, A., Degrave, W.M., Da Silveira, J.F.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 1997
Materias:
Chromosome mapping
Clone CL Brener
Genetic map
Genome project
Molecular karyotype
Trypanosoma cruzi
Trypanosoma
animal
chromosome map
cloning
genetics
genome
karyotyping
review
Animals
Chromosome Mapping
Cloning, Organism
Genome, Protozoan
Karyotyping
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00740276_v92_n6_p821_Santos
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_00740276_v92_n6_p821_Santos
record_format dspace
spelling paperaa:paper_00740276_v92_n6_p821_Santos2023-06-12T16:45:48Z The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener Mem. Inst. Oswaldo Cruz 1997;92(6):821-828 Santos, M.R.M. Cano, M.I. Schijman, A. Lorenzi, H. Vázquez, M. Levin, M.J. Ramirez, J.L. Brandão, A. Degrave, W.M. Da Silveira, J.F. Chromosome mapping Clone CL Brener Genetic map Genome project Molecular karyotype Trypanosoma cruzi Trypanosoma Trypanosoma cruzi animal chromosome map cloning genetics genome karyotyping review Trypanosoma cruzi Animals Chromosome Mapping Cloning, Organism Genome, Protozoan Karyotyping Trypanosoma cruzi By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes. Fil:Schijman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lorenzi, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vázquez, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00740276_v92_n6_p821_Santos
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic Chromosome mapping
Clone CL Brener
Genetic map
Genome project
Molecular karyotype
Trypanosoma cruzi
Trypanosoma
Trypanosoma cruzi
animal
chromosome map
cloning
genetics
genome
karyotyping
review
Trypanosoma cruzi
Animals
Chromosome Mapping
Cloning, Organism
Genome, Protozoan
Karyotyping
Trypanosoma cruzi
spellingShingle Chromosome mapping
Clone CL Brener
Genetic map
Genome project
Molecular karyotype
Trypanosoma cruzi
Trypanosoma
Trypanosoma cruzi
animal
chromosome map
cloning
genetics
genome
karyotyping
review
Trypanosoma cruzi
Animals
Chromosome Mapping
Cloning, Organism
Genome, Protozoan
Karyotyping
Trypanosoma cruzi
Santos, M.R.M.
Cano, M.I.
Schijman, A.
Lorenzi, H.
Vázquez, M.
Levin, M.J.
Ramirez, J.L.
Brandão, A.
Degrave, W.M.
Da Silveira, J.F.
The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
topic_facet Chromosome mapping
Clone CL Brener
Genetic map
Genome project
Molecular karyotype
Trypanosoma cruzi
Trypanosoma
Trypanosoma cruzi
animal
chromosome map
cloning
genetics
genome
karyotyping
review
Trypanosoma cruzi
Animals
Chromosome Mapping
Cloning, Organism
Genome, Protozoan
Karyotyping
Trypanosoma cruzi
description By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes.
format Artículo
Artículo
publishedVersion
author Santos, M.R.M.
Cano, M.I.
Schijman, A.
Lorenzi, H.
Vázquez, M.
Levin, M.J.
Ramirez, J.L.
Brandão, A.
Degrave, W.M.
Da Silveira, J.F.
author_facet Santos, M.R.M.
Cano, M.I.
Schijman, A.
Lorenzi, H.
Vázquez, M.
Levin, M.J.
Ramirez, J.L.
Brandão, A.
Degrave, W.M.
Da Silveira, J.F.
author_sort Santos, M.R.M.
title The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_short The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_full The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_fullStr The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_full_unstemmed The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_sort trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone cl brener
publishDate 1997
url http://hdl.handle.net/20.500.12110/paper_00740276_v92_n6_p821_Santos
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