Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins
The trypanocidal action of green tea catechins against two different developmental stages of Trypanosoma cruzi is reported for the first time. This activity was assayed with the nonproliferative bloodstream trypomastigote and with the intracellular replicative amastigote parasite forms. An ethyl ace...
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| Autores principales: | , , , , , , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
2004
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00664804_v48_n1_p69_Paveto |
| Aporte de: |
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paperaa:paper_00664804_v48_n1_p69_Paveto |
|---|---|
| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
acetic acid ethyl ester arginine kinase catechin catechin gallate epicatechin epicatechin gallate epigallocatechin epigallocatechin gallate flavanol derivative gallocatechin gallocatechin gallate green tea extract organic solvent polyphenol derivative unclassified drug amastigote animal cell animal experiment animal model antiprotozoal activity aqueous solution article bactericidal activity blood blood flow column chromatography concentration response controlled study developmental stage diode drug determination drug effect drug efficacy drug identification drug mechanism drug purification drug screening energy metabolism enzyme inactivation enzyme inhibition fractionation gas chromatography high performance liquid chromatography lysis male mass spectrometry minimum inhibitory concentration mouse nonhuman plant leaf priority journal protein function protozoal infection tea Trypanosoma cruzi trypomastigote Vero cell Animals Arginine Kinase Camellia Catechin Cercopithecus aethiops Chagas Disease Chromatography, Gas Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Indicators and Reagents Male Mice Mice, Inbred BALB C Plant Extracts Trypanocidal Agents Trypanosoma cruzi Vero Cells |
| spellingShingle |
acetic acid ethyl ester arginine kinase catechin catechin gallate epicatechin epicatechin gallate epigallocatechin epigallocatechin gallate flavanol derivative gallocatechin gallocatechin gallate green tea extract organic solvent polyphenol derivative unclassified drug amastigote animal cell animal experiment animal model antiprotozoal activity aqueous solution article bactericidal activity blood blood flow column chromatography concentration response controlled study developmental stage diode drug determination drug effect drug efficacy drug identification drug mechanism drug purification drug screening energy metabolism enzyme inactivation enzyme inhibition fractionation gas chromatography high performance liquid chromatography lysis male mass spectrometry minimum inhibitory concentration mouse nonhuman plant leaf priority journal protein function protozoal infection tea Trypanosoma cruzi trypomastigote Vero cell Animals Arginine Kinase Camellia Catechin Cercopithecus aethiops Chagas Disease Chromatography, Gas Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Indicators and Reagents Male Mice Mice, Inbred BALB C Plant Extracts Trypanocidal Agents Trypanosoma cruzi Vero Cells Paveto, C. Güida, M.C. Esteva, M.I. Martino, V. Coussio, J. Flawiá, M.M. Torres, H.N. Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins |
| topic_facet |
acetic acid ethyl ester arginine kinase catechin catechin gallate epicatechin epicatechin gallate epigallocatechin epigallocatechin gallate flavanol derivative gallocatechin gallocatechin gallate green tea extract organic solvent polyphenol derivative unclassified drug amastigote animal cell animal experiment animal model antiprotozoal activity aqueous solution article bactericidal activity blood blood flow column chromatography concentration response controlled study developmental stage diode drug determination drug effect drug efficacy drug identification drug mechanism drug purification drug screening energy metabolism enzyme inactivation enzyme inhibition fractionation gas chromatography high performance liquid chromatography lysis male mass spectrometry minimum inhibitory concentration mouse nonhuman plant leaf priority journal protein function protozoal infection tea Trypanosoma cruzi trypomastigote Vero cell Animals Arginine Kinase Camellia Catechin Cercopithecus aethiops Chagas Disease Chromatography, Gas Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Indicators and Reagents Male Mice Mice, Inbred BALB C Plant Extracts Trypanocidal Agents Trypanosoma cruzi Vero Cells |
| description |
The trypanocidal action of green tea catechins against two different developmental stages of Trypanosoma cruzi is reported for the first time. This activity was assayed with the nonproliferative bloodstream trypomastigote and with the intracellular replicative amastigote parasite forms. An ethyl acetate fraction from Camellia sinensis green tea leaves, which contains most of the polyphenolic compounds and the maximal trypanocidal activity, was obtained by fractionation of the aqueous extract with organic solvents. The active compounds present in this extract were further purified by LH-20 column chromatography and were identified by high-performance liquid chromatography analysis with a photo diode array detector and gas chromatography coupled to mass spectroscopy. The following flavan-3-ols derivatives, known as catechins, were identified: catechin, epicatechin, gallocatechin, epigallocatechin, catechin gallate, epicatechin gallate, gallocatechin gallate, and epigallocatechin gallate. The purified compounds lysed more than 50% of the parasites present in the blood of infected BALB/c mice at concentrations as low as 0.12 to 85 pM. The most active compounds were gallocatechin gallate and epigallocatechin gallate, with minimal bactericidal concentrations that inhibited 50% of isolates tested of 0.12 and 0.53 pM, respectively. The number of amastigotes in infected Vero cells decreased by 50% in the presence of each of these compounds at 100 nM. The effects of the catechins on the recombinant T. cruzi arginine kinase, a key enzyme in the energy metabolism of the parasite, were assayed. The activity of this enzyme was inhibited by about 50% by nanomolar concentrations of catechin gallate or gallocatechin gallate, whereas the other members of the group were less effective. On the basis of these results, we suggest that these compounds could be used to sterilize blood and, eventually, as therapeutic agents for Chagas' disease. |
| format |
Artículo Artículo publishedVersion |
| author |
Paveto, C. Güida, M.C. Esteva, M.I. Martino, V. Coussio, J. Flawiá, M.M. Torres, H.N. |
| author_facet |
Paveto, C. Güida, M.C. Esteva, M.I. Martino, V. Coussio, J. Flawiá, M.M. Torres, H.N. |
| author_sort |
Paveto, C. |
| title |
Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins |
| title_short |
Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins |
| title_full |
Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins |
| title_fullStr |
Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins |
| title_full_unstemmed |
Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins |
| title_sort |
anti-trypanosoma cruzi activity of green tea (camellia sinensis) catechins |
| publishDate |
2004 |
| url |
http://hdl.handle.net/20.500.12110/paper_00664804_v48_n1_p69_Paveto |
| work_keys_str_mv |
AT pavetoc antitrypanosomacruziactivityofgreenteacamelliasinensiscatechins AT guidamc antitrypanosomacruziactivityofgreenteacamelliasinensiscatechins AT estevami antitrypanosomacruziactivityofgreenteacamelliasinensiscatechins AT martinov antitrypanosomacruziactivityofgreenteacamelliasinensiscatechins AT coussioj antitrypanosomacruziactivityofgreenteacamelliasinensiscatechins AT flawiamm antitrypanosomacruziactivityofgreenteacamelliasinensiscatechins AT torreshn antitrypanosomacruziactivityofgreenteacamelliasinensiscatechins |
| _version_ |
1769810102860120064 |
| spelling |
paperaa:paper_00664804_v48_n1_p69_Paveto2023-06-12T16:45:42Z Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins Antimicrob. Agents Chemother. 2004;48(1):69-74 Paveto, C. Güida, M.C. Esteva, M.I. Martino, V. Coussio, J. Flawiá, M.M. Torres, H.N. acetic acid ethyl ester arginine kinase catechin catechin gallate epicatechin epicatechin gallate epigallocatechin epigallocatechin gallate flavanol derivative gallocatechin gallocatechin gallate green tea extract organic solvent polyphenol derivative unclassified drug amastigote animal cell animal experiment animal model antiprotozoal activity aqueous solution article bactericidal activity blood blood flow column chromatography concentration response controlled study developmental stage diode drug determination drug effect drug efficacy drug identification drug mechanism drug purification drug screening energy metabolism enzyme inactivation enzyme inhibition fractionation gas chromatography high performance liquid chromatography lysis male mass spectrometry minimum inhibitory concentration mouse nonhuman plant leaf priority journal protein function protozoal infection tea Trypanosoma cruzi trypomastigote Vero cell Animals Arginine Kinase Camellia Catechin Cercopithecus aethiops Chagas Disease Chromatography, Gas Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Indicators and Reagents Male Mice Mice, Inbred BALB C Plant Extracts Trypanocidal Agents Trypanosoma cruzi Vero Cells The trypanocidal action of green tea catechins against two different developmental stages of Trypanosoma cruzi is reported for the first time. This activity was assayed with the nonproliferative bloodstream trypomastigote and with the intracellular replicative amastigote parasite forms. An ethyl acetate fraction from Camellia sinensis green tea leaves, which contains most of the polyphenolic compounds and the maximal trypanocidal activity, was obtained by fractionation of the aqueous extract with organic solvents. The active compounds present in this extract were further purified by LH-20 column chromatography and were identified by high-performance liquid chromatography analysis with a photo diode array detector and gas chromatography coupled to mass spectroscopy. The following flavan-3-ols derivatives, known as catechins, were identified: catechin, epicatechin, gallocatechin, epigallocatechin, catechin gallate, epicatechin gallate, gallocatechin gallate, and epigallocatechin gallate. The purified compounds lysed more than 50% of the parasites present in the blood of infected BALB/c mice at concentrations as low as 0.12 to 85 pM. The most active compounds were gallocatechin gallate and epigallocatechin gallate, with minimal bactericidal concentrations that inhibited 50% of isolates tested of 0.12 and 0.53 pM, respectively. The number of amastigotes in infected Vero cells decreased by 50% in the presence of each of these compounds at 100 nM. The effects of the catechins on the recombinant T. cruzi arginine kinase, a key enzyme in the energy metabolism of the parasite, were assayed. The activity of this enzyme was inhibited by about 50% by nanomolar concentrations of catechin gallate or gallocatechin gallate, whereas the other members of the group were less effective. On the basis of these results, we suggest that these compounds could be used to sterilize blood and, eventually, as therapeutic agents for Chagas' disease. Fil:Paveto, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Güida, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00664804_v48_n1_p69_Paveto |