A cytoplasmically inherited barley mutant is defective in photosystem I assembly due to a temperature-sensitive defect in ycf3 splicing
A cytoplasmically inherited chlorophyll-deficient mutant of barley (Hordeum vulgare) termed cytoplasmic line 3 (CL3), displaying a viridis (homogeneously light-green colored) phenotype, has been previously shown to be affected by elevated temperatures. In this article, biochemical, biophysical, and...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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2009
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00320889_v151_n4_p1802_Landau |
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paperaa:paper_00320889_v151_n4_p1802_Landau2023-06-12T16:45:27Z A cytoplasmically inherited barley mutant is defective in photosystem I assembly due to a temperature-sensitive defect in ycf3 splicing Plant Physiol. 2009;151(4):1802-1811 Landau, A.M. Lokstein, H. Scheller, H.V. Lainez, V. Maldonado, S. Prina, A.R. messenger RNA pigment plant extract vegetable protein article barley chloroplast cytoplasm gene expression regulation genetics germination growth, development and aging immunoblotting inheritance metabolism mutation nucleotide sequence phenotype photochemistry photosynthesis photosystem I plant gene RNA splicing seedling spectrofluorometry temperature thylakoid ultrastructure Base Sequence Chloroplasts Cytoplasm Gene Expression Regulation, Plant Genes, Plant Germination Hordeum Immunoblotting Inheritance Patterns Mutation Phenotype Photochemistry Photosynthesis Photosystem I Protein Complex Pigments, Biological Plant Extracts Plant Proteins RNA Splicing RNA, Messenger Seedling Spectrometry, Fluorescence Temperature Thylakoids Hordeum Hordeum vulgare A cytoplasmically inherited chlorophyll-deficient mutant of barley (Hordeum vulgare) termed cytoplasmic line 3 (CL3), displaying a viridis (homogeneously light-green colored) phenotype, has been previously shown to be affected by elevated temperatures. In this article, biochemical, biophysical, and molecular approaches were used to study the CL3 mutant under different temperature and light conditions. The results lead to the conclusion that an impaired assembly of photosystem I (PSI) under higher temperatures and certain light conditions is the primary cause of the CL3 phenotype. Compromised splicing of ycf3 transcripts, particularly at elevated temperature, resulting from a mutation in a noncoding region (intron 1) in the mutant ycf3 gene results in a defective synthesis of Ycf3, which is a chaperone involved in PSI assembly. The defective PSI assembly causes severe photoinhibition and degradation of PSII. © 2009 American Society of Plant Biologists. Fil:Landau, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lainez, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00320889_v151_n4_p1802_Landau |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
messenger RNA pigment plant extract vegetable protein article barley chloroplast cytoplasm gene expression regulation genetics germination growth, development and aging immunoblotting inheritance metabolism mutation nucleotide sequence phenotype photochemistry photosynthesis photosystem I plant gene RNA splicing seedling spectrofluorometry temperature thylakoid ultrastructure Base Sequence Chloroplasts Cytoplasm Gene Expression Regulation, Plant Genes, Plant Germination Hordeum Immunoblotting Inheritance Patterns Mutation Phenotype Photochemistry Photosynthesis Photosystem I Protein Complex Pigments, Biological Plant Extracts Plant Proteins RNA Splicing RNA, Messenger Seedling Spectrometry, Fluorescence Temperature Thylakoids Hordeum Hordeum vulgare |
| spellingShingle |
messenger RNA pigment plant extract vegetable protein article barley chloroplast cytoplasm gene expression regulation genetics germination growth, development and aging immunoblotting inheritance metabolism mutation nucleotide sequence phenotype photochemistry photosynthesis photosystem I plant gene RNA splicing seedling spectrofluorometry temperature thylakoid ultrastructure Base Sequence Chloroplasts Cytoplasm Gene Expression Regulation, Plant Genes, Plant Germination Hordeum Immunoblotting Inheritance Patterns Mutation Phenotype Photochemistry Photosynthesis Photosystem I Protein Complex Pigments, Biological Plant Extracts Plant Proteins RNA Splicing RNA, Messenger Seedling Spectrometry, Fluorescence Temperature Thylakoids Hordeum Hordeum vulgare Landau, A.M. Lokstein, H. Scheller, H.V. Lainez, V. Maldonado, S. Prina, A.R. A cytoplasmically inherited barley mutant is defective in photosystem I assembly due to a temperature-sensitive defect in ycf3 splicing |
| topic_facet |
messenger RNA pigment plant extract vegetable protein article barley chloroplast cytoplasm gene expression regulation genetics germination growth, development and aging immunoblotting inheritance metabolism mutation nucleotide sequence phenotype photochemistry photosynthesis photosystem I plant gene RNA splicing seedling spectrofluorometry temperature thylakoid ultrastructure Base Sequence Chloroplasts Cytoplasm Gene Expression Regulation, Plant Genes, Plant Germination Hordeum Immunoblotting Inheritance Patterns Mutation Phenotype Photochemistry Photosynthesis Photosystem I Protein Complex Pigments, Biological Plant Extracts Plant Proteins RNA Splicing RNA, Messenger Seedling Spectrometry, Fluorescence Temperature Thylakoids Hordeum Hordeum vulgare |
| description |
A cytoplasmically inherited chlorophyll-deficient mutant of barley (Hordeum vulgare) termed cytoplasmic line 3 (CL3), displaying a viridis (homogeneously light-green colored) phenotype, has been previously shown to be affected by elevated temperatures. In this article, biochemical, biophysical, and molecular approaches were used to study the CL3 mutant under different temperature and light conditions. The results lead to the conclusion that an impaired assembly of photosystem I (PSI) under higher temperatures and certain light conditions is the primary cause of the CL3 phenotype. Compromised splicing of ycf3 transcripts, particularly at elevated temperature, resulting from a mutation in a noncoding region (intron 1) in the mutant ycf3 gene results in a defective synthesis of Ycf3, which is a chaperone involved in PSI assembly. The defective PSI assembly causes severe photoinhibition and degradation of PSII. © 2009 American Society of Plant Biologists. |
| format |
Artículo Artículo publishedVersion |
| author |
Landau, A.M. Lokstein, H. Scheller, H.V. Lainez, V. Maldonado, S. Prina, A.R. |
| author_facet |
Landau, A.M. Lokstein, H. Scheller, H.V. Lainez, V. Maldonado, S. Prina, A.R. |
| author_sort |
Landau, A.M. |
| title |
A cytoplasmically inherited barley mutant is defective in photosystem I assembly due to a temperature-sensitive defect in ycf3 splicing |
| title_short |
A cytoplasmically inherited barley mutant is defective in photosystem I assembly due to a temperature-sensitive defect in ycf3 splicing |
| title_full |
A cytoplasmically inherited barley mutant is defective in photosystem I assembly due to a temperature-sensitive defect in ycf3 splicing |
| title_fullStr |
A cytoplasmically inherited barley mutant is defective in photosystem I assembly due to a temperature-sensitive defect in ycf3 splicing |
| title_full_unstemmed |
A cytoplasmically inherited barley mutant is defective in photosystem I assembly due to a temperature-sensitive defect in ycf3 splicing |
| title_sort |
cytoplasmically inherited barley mutant is defective in photosystem i assembly due to a temperature-sensitive defect in ycf3 splicing |
| publishDate |
2009 |
| url |
http://hdl.handle.net/20.500.12110/paper_00320889_v151_n4_p1802_Landau |
| work_keys_str_mv |
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