The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato (Solanum lycopersicum), LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pisti...
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| Lenguaje: | Inglés |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00320889_v148_n3_p1368_Zhang |
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paperaa:paper_00320889_v148_n3_p1368_Zhang2023-06-12T16:45:27Z The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth Plant Physiol. 2008;148(3):1368-1379 Zhang, D. Wengier, D. Shuai, B. Gui, C.-P. Muschietti, J. McCormick, S. Tang, W.-H. hybrid protein primer DNA protein kinase article enzymology germination growth, development and aging metabolism molecular genetics nucleotide sequence physiology pollen polymerase chain reaction signal transduction tomato transgenic plant Base Sequence DNA Primers Germination Lycopersicon esculentum Molecular Sequence Data Plants, Genetically Modified Pollen Polymerase Chain Reaction Protein Kinases Recombinant Fusion Proteins Signal Transduction Lycopersicon esculentum Magnoliophyta Solanum In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato (Solanum lycopersicum), LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pistil communication. Here we show that reduced expression of LePRK2 affects four aspects of pollen germination and tube growth. First, the percentage of pollen that germinates is reduced, and the time window for competence to germinate is also shorter. Second, the pollen tube growth rate is reduced both in vitro and in the pistil. Third, tip-localized superoxide production by pollen tubes cannot be increased by exogenous calcium ions. Fourth, pollen tubes have defects in responses to style extract component (STIL), an extracellular growth-promoting signal from the pistil. Pollen tubes transiently overexpressing LePRK2-fluorescent protein fusions had slightly wider tips, whereas pollen tubes coexpressing LePRK2 and its cytoplasmic partner protein KPP (a Rop-GEF) had much wider tips. Together these results show that LePRK2 positively regulates pollen germination and tube growth and is involved in transducing responses to extracellular growth-promoting signals. © 2008 American Society of Plant Biologists. Fil:Wengier, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muschietti, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00320889_v148_n3_p1368_Zhang |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
hybrid protein primer DNA protein kinase article enzymology germination growth, development and aging metabolism molecular genetics nucleotide sequence physiology pollen polymerase chain reaction signal transduction tomato transgenic plant Base Sequence DNA Primers Germination Lycopersicon esculentum Molecular Sequence Data Plants, Genetically Modified Pollen Polymerase Chain Reaction Protein Kinases Recombinant Fusion Proteins Signal Transduction Lycopersicon esculentum Magnoliophyta Solanum |
| spellingShingle |
hybrid protein primer DNA protein kinase article enzymology germination growth, development and aging metabolism molecular genetics nucleotide sequence physiology pollen polymerase chain reaction signal transduction tomato transgenic plant Base Sequence DNA Primers Germination Lycopersicon esculentum Molecular Sequence Data Plants, Genetically Modified Pollen Polymerase Chain Reaction Protein Kinases Recombinant Fusion Proteins Signal Transduction Lycopersicon esculentum Magnoliophyta Solanum Zhang, D. Wengier, D. Shuai, B. Gui, C.-P. Muschietti, J. McCormick, S. Tang, W.-H. The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth |
| topic_facet |
hybrid protein primer DNA protein kinase article enzymology germination growth, development and aging metabolism molecular genetics nucleotide sequence physiology pollen polymerase chain reaction signal transduction tomato transgenic plant Base Sequence DNA Primers Germination Lycopersicon esculentum Molecular Sequence Data Plants, Genetically Modified Pollen Polymerase Chain Reaction Protein Kinases Recombinant Fusion Proteins Signal Transduction Lycopersicon esculentum Magnoliophyta Solanum |
| description |
In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato (Solanum lycopersicum), LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pistil communication. Here we show that reduced expression of LePRK2 affects four aspects of pollen germination and tube growth. First, the percentage of pollen that germinates is reduced, and the time window for competence to germinate is also shorter. Second, the pollen tube growth rate is reduced both in vitro and in the pistil. Third, tip-localized superoxide production by pollen tubes cannot be increased by exogenous calcium ions. Fourth, pollen tubes have defects in responses to style extract component (STIL), an extracellular growth-promoting signal from the pistil. Pollen tubes transiently overexpressing LePRK2-fluorescent protein fusions had slightly wider tips, whereas pollen tubes coexpressing LePRK2 and its cytoplasmic partner protein KPP (a Rop-GEF) had much wider tips. Together these results show that LePRK2 positively regulates pollen germination and tube growth and is involved in transducing responses to extracellular growth-promoting signals. © 2008 American Society of Plant Biologists. |
| format |
Artículo Artículo publishedVersion |
| author |
Zhang, D. Wengier, D. Shuai, B. Gui, C.-P. Muschietti, J. McCormick, S. Tang, W.-H. |
| author_facet |
Zhang, D. Wengier, D. Shuai, B. Gui, C.-P. Muschietti, J. McCormick, S. Tang, W.-H. |
| author_sort |
Zhang, D. |
| title |
The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth |
| title_short |
The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth |
| title_full |
The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth |
| title_fullStr |
The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth |
| title_full_unstemmed |
The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth |
| title_sort |
pollen receptor kinase leprk2 mediates growth-promoting signals and positively regulates pollen germination and tube growth |
| publishDate |
2008 |
| url |
http://hdl.handle.net/20.500.12110/paper_00320889_v148_n3_p1368_Zhang |
| work_keys_str_mv |
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