Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms

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We previously demonstrated that extracellular bacterial DNA activates neutrophils through a CpG- and TLR9-independent mechanism. Biofilms are microbial communities enclosed in a polymeric matrix that play a critical role in the pathogenesis of many infectious diseases. Because extracellular DNA is a...

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Autores principales:	Fuxman Bass, J.I., Russo, D.M., Gabelloni, M.L., Geffner, J.R., Giordano, M., Catalano, M., Zorreguieta, Á., Trevani, A.S.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2010
Materias:	CD11b antigen CD18 antigen CD66b antigen deoxyribonuclease I DNA interleukin 1beta interleukin 8 lymphocyte antigen unclassified drug bacterial DNA cytokine article bacterial strain biofilm controlled study cytokine release degradation extracellular matrix human leukocyte activation nonhuman phagocytosis priority journal Pseudomonas aeruginosa upregulation biosynthesis chemistry confocal microscopy extracellular fluid growth, development and aging immunology metabolism microbiology neutrophil physiology Biofilms Cytokines DNA, Bacterial Extracellular Fluid Humans Microscopy, Confocal Neutrophil Activation Neutrophils
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00221767_v184_n11_p6386_FuxmanBass
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paperaa:paper_00221767_v184_n11_p6386_FuxmanBass2023-06-12T16:43:58Z Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms J. Immunol. 2010;184(11):6386-6395 Fuxman Bass, J.I. Russo, D.M. Gabelloni, M.L. Geffner, J.R. Giordano, M. Catalano, M. Zorreguieta, Á. Trevani, A.S. CD11b antigen CD18 antigen CD66b antigen deoxyribonuclease I DNA interleukin 1beta interleukin 8 lymphocyte antigen unclassified drug bacterial DNA cytokine article bacterial strain biofilm controlled study cytokine release degradation extracellular matrix human leukocyte activation nonhuman phagocytosis priority journal Pseudomonas aeruginosa upregulation biosynthesis chemistry confocal microscopy extracellular fluid growth, development and aging immunology leukocyte activation metabolism microbiology neutrophil physiology Biofilms Cytokines DNA, Bacterial Extracellular Fluid Humans Microscopy, Confocal Neutrophil Activation Neutrophils Pseudomonas aeruginosa We previously demonstrated that extracellular bacterial DNA activates neutrophils through a CpG- and TLR9-independent mechanism. Biofilms are microbial communities enclosed in a polymeric matrix that play a critical role in the pathogenesis of many infectious diseases. Because extracellular DNA is a key component of biofilms of different bacterial species, the aim of this study was to determine whether it plays a role in the ability of biofilms to induce human neutrophil activation. We found that degradation of matrix extracellular DNA with DNase I markedly reduced the capacity of Pseudomonas aeruginosa biofilms to induce the release of the neutrophil proinflammatory cytokines IL-8 and IL-1β (>75%); reduced the upregulation of neutrophil activation markers CD18, CD11b, and CD66b (p < 0.001); reduced the number of bacteria phagocytosed per neutrophil contacting the biofilm; and reduced the production of neutrophil extracellular traps. Consistent with these findings, we found that biofilms formed by the lasI rhlI P. aeruginosa mutant strain, exhibiting a very low content of matrix extracellular DNA, displayed a lower capacity to stimulate the release of proinflammatory cytokines by neutrophils, which was not decreased further by DNase I treatment. Together, our findings support that matrix extracellular DNA is a major proinflammatory component of P. aeruginosa biofilms. Copyright © 2010 by The American Association of Immunologists, Inc. Fil:Fuxman Bass, J.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Russo, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gabelloni, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giordano, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zorreguieta, Á. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Trevani, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00221767_v184_n11_p6386_FuxmanBass
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	CD11b antigen CD18 antigen CD66b antigen deoxyribonuclease I DNA interleukin 1beta interleukin 8 lymphocyte antigen unclassified drug bacterial DNA cytokine article bacterial strain biofilm controlled study cytokine release degradation extracellular matrix human leukocyte activation nonhuman phagocytosis priority journal Pseudomonas aeruginosa upregulation biosynthesis chemistry confocal microscopy extracellular fluid growth, development and aging immunology leukocyte activation metabolism microbiology neutrophil physiology Biofilms Cytokines DNA, Bacterial Extracellular Fluid Humans Microscopy, Confocal Neutrophil Activation Neutrophils Pseudomonas aeruginosa
spellingShingle	CD11b antigen CD18 antigen CD66b antigen deoxyribonuclease I DNA interleukin 1beta interleukin 8 lymphocyte antigen unclassified drug bacterial DNA cytokine article bacterial strain biofilm controlled study cytokine release degradation extracellular matrix human leukocyte activation nonhuman phagocytosis priority journal Pseudomonas aeruginosa upregulation biosynthesis chemistry confocal microscopy extracellular fluid growth, development and aging immunology leukocyte activation metabolism microbiology neutrophil physiology Biofilms Cytokines DNA, Bacterial Extracellular Fluid Humans Microscopy, Confocal Neutrophil Activation Neutrophils Pseudomonas aeruginosa Fuxman Bass, J.I. Russo, D.M. Gabelloni, M.L. Geffner, J.R. Giordano, M. Catalano, M. Zorreguieta, Á. Trevani, A.S. Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms
topic_facet	CD11b antigen CD18 antigen CD66b antigen deoxyribonuclease I DNA interleukin 1beta interleukin 8 lymphocyte antigen unclassified drug bacterial DNA cytokine article bacterial strain biofilm controlled study cytokine release degradation extracellular matrix human leukocyte activation nonhuman phagocytosis priority journal Pseudomonas aeruginosa upregulation biosynthesis chemistry confocal microscopy extracellular fluid growth, development and aging immunology leukocyte activation metabolism microbiology neutrophil physiology Biofilms Cytokines DNA, Bacterial Extracellular Fluid Humans Microscopy, Confocal Neutrophil Activation Neutrophils Pseudomonas aeruginosa
description	We previously demonstrated that extracellular bacterial DNA activates neutrophils through a CpG- and TLR9-independent mechanism. Biofilms are microbial communities enclosed in a polymeric matrix that play a critical role in the pathogenesis of many infectious diseases. Because extracellular DNA is a key component of biofilms of different bacterial species, the aim of this study was to determine whether it plays a role in the ability of biofilms to induce human neutrophil activation. We found that degradation of matrix extracellular DNA with DNase I markedly reduced the capacity of Pseudomonas aeruginosa biofilms to induce the release of the neutrophil proinflammatory cytokines IL-8 and IL-1β (>75%); reduced the upregulation of neutrophil activation markers CD18, CD11b, and CD66b (p < 0.001); reduced the number of bacteria phagocytosed per neutrophil contacting the biofilm; and reduced the production of neutrophil extracellular traps. Consistent with these findings, we found that biofilms formed by the lasI rhlI P. aeruginosa mutant strain, exhibiting a very low content of matrix extracellular DNA, displayed a lower capacity to stimulate the release of proinflammatory cytokines by neutrophils, which was not decreased further by DNase I treatment. Together, our findings support that matrix extracellular DNA is a major proinflammatory component of P. aeruginosa biofilms. Copyright © 2010 by The American Association of Immunologists, Inc.
format	Artículo Artículo publishedVersion
author	Fuxman Bass, J.I. Russo, D.M. Gabelloni, M.L. Geffner, J.R. Giordano, M. Catalano, M. Zorreguieta, Á. Trevani, A.S.
author_facet	Fuxman Bass, J.I. Russo, D.M. Gabelloni, M.L. Geffner, J.R. Giordano, M. Catalano, M. Zorreguieta, Á. Trevani, A.S.
author_sort	Fuxman Bass, J.I.
title	Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms
title_short	Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms
title_full	Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms
title_fullStr	Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms
title_full_unstemmed	Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms
title_sort	extracellular dna: a major proinflammatory component of pseudomonas aeruginosa biofilms
publishDate	2010
url	http://hdl.handle.net/20.500.12110/paper_00221767_v184_n11_p6386_FuxmanBass
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_version_	1769810088882601984

Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms

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