Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.

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Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene...

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Autores principales:	Conforte, V.P., Echeverria, M., Sánchez, C., Ugalde, R.A., Menéndez, A.B., Lepek, V.C.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2010
Materias:	ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root Lotus corniculatus var. japonicus Lotus tenuis Carbon-Carbon Lyases Plant Roots Symbiosis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00221260_v56_n4_p331_Conforte
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_00221260_v56_n4_p331_Conforte
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spelling	paperaa:paper_00221260_v56_n4_p331_Conforte2023-06-12T16:43:53Z Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. J. Gen. Appl. Microbiol. 2010;56(4):331-338 Conforte, V.P. Echeverria, M. Sánchez, C. Ugalde, R.A. Menéndez, A.B. Lepek, V.C. ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume Lotus Mesorhizobium loti nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root symbiosis Lotus corniculatus var. japonicus Lotus tenuis Mesorhizobium loti Alphaproteobacteria Carbon-Carbon Lyases Lotus Plant Roots Symbiosis Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene encoding ACC deaminase, but this gene is under the activity of the NifA-RpoN- dependent promoter; thus, it is only expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was integrated into the M. loti chromosome under a constitutive promoter activity. The resulting strain induced the formation of a higher number of nodules and was more competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results suggest that the introduction of the ACC deaminase activity within M. loti in a constitutive way could be a novel strategy to increase nodulation competitiveness of the bacteria, which could be useful for the forage inoculants industry. Fil:Conforte, V.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Echeverria, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ugalde, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Menéndez, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lepek, V.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00221260_v56_n4_p331_Conforte
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume Lotus Mesorhizobium loti nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root symbiosis Lotus corniculatus var. japonicus Lotus tenuis Mesorhizobium loti Alphaproteobacteria Carbon-Carbon Lyases Lotus Plant Roots Symbiosis
spellingShingle	ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume Lotus Mesorhizobium loti nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root symbiosis Lotus corniculatus var. japonicus Lotus tenuis Mesorhizobium loti Alphaproteobacteria Carbon-Carbon Lyases Lotus Plant Roots Symbiosis Conforte, V.P. Echeverria, M. Sánchez, C. Ugalde, R.A. Menéndez, A.B. Lepek, V.C. Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
topic_facet	ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume Lotus Mesorhizobium loti nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root symbiosis Lotus corniculatus var. japonicus Lotus tenuis Mesorhizobium loti Alphaproteobacteria Carbon-Carbon Lyases Lotus Plant Roots Symbiosis
description	Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene encoding ACC deaminase, but this gene is under the activity of the NifA-RpoN- dependent promoter; thus, it is only expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was integrated into the M. loti chromosome under a constitutive promoter activity. The resulting strain induced the formation of a higher number of nodules and was more competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results suggest that the introduction of the ACC deaminase activity within M. loti in a constitutive way could be a novel strategy to increase nodulation competitiveness of the bacteria, which could be useful for the forage inoculants industry.
format	Artículo Artículo publishedVersion
author	Conforte, V.P. Echeverria, M. Sánchez, C. Ugalde, R.A. Menéndez, A.B. Lepek, V.C.
author_facet	Conforte, V.P. Echeverria, M. Sánchez, C. Ugalde, R.A. Menéndez, A.B. Lepek, V.C.
author_sort	Conforte, V.P.
title	Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
title_short	Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
title_full	Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
title_fullStr	Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
title_full_unstemmed	Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
title_sort	engineered acc deaminase-expressing free-living cells of mesorhizobium loti show increased nodulation efficiency and competitiveness on lotus spp.
publishDate	2010
url	http://hdl.handle.net/20.500.12110/paper_00221260_v56_n4_p331_Conforte
work_keys_str_mv	AT confortevp engineeredaccdeaminaseexpressingfreelivingcellsofmesorhizobiumlotishowincreasednodulationefficiencyandcompetitivenessonlotusspp AT echeverriam engineeredaccdeaminaseexpressingfreelivingcellsofmesorhizobiumlotishowincreasednodulationefficiencyandcompetitivenessonlotusspp AT sanchezc engineeredaccdeaminaseexpressingfreelivingcellsofmesorhizobiumlotishowincreasednodulationefficiencyandcompetitivenessonlotusspp AT ugaldera engineeredaccdeaminaseexpressingfreelivingcellsofmesorhizobiumlotishowincreasednodulationefficiencyandcompetitivenessonlotusspp AT menendezab engineeredaccdeaminaseexpressingfreelivingcellsofmesorhizobiumlotishowincreasednodulationefficiencyandcompetitivenessonlotusspp AT lepekvc engineeredaccdeaminaseexpressingfreelivingcellsofmesorhizobiumlotishowincreasednodulationefficiencyandcompetitivenessonlotusspp
_version_	1769810164710375424

Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.

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