Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors
The effects of dopamine (DA) and dopaminergic agonists and antagonists on ion transport were studied in isolated perfused gills of the crab Chasmagnathus granulatus. DA applied under steady state conditions (perfusion with hemolymph-like saline) produced a transient increase of the transepithelial p...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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2006
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00220949_v209_n14_p2785_Genovese |
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paperaa:paper_00220949_v209_n14_p2785_Genovese2023-06-12T16:43:43Z Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors J. Exp. Biol. 2006;209(14):2785-2793 Genovese, G. Senek, M. Ortiz, N. Regueira, M. Towle, D.W. Tresguerres, M. Luquet, C.M. cAMP Chasmagnathus granulatus Crab Dopamine antagonist Dopamine receptor dopamine agonist Na +/K+-ATPase Okadaic acid Transepithelial potential differences adenosine triphosphatase (potassium sodium) cyclic AMP dependent protein kinase dopamine 1 receptor dopamine 2 receptor okadaic acid theophylline animal article Brachyura gill ion transport male metabolism osmolarity physiology signal transduction Animals Brachyura Cyclic AMP-Dependent Protein Kinases Gills Ion Transport Male Na(+)-K(+)-Exchanging ATPase Okadaic Acid Osmolar Concentration Receptors, Dopamine D1 Receptors, Dopamine D2 Signal Transduction Theophylline Chasmagnathus granulata Decapoda (Crustacea) Hexapoda Insecta Vertebrata The effects of dopamine (DA) and dopaminergic agonists and antagonists on ion transport were studied in isolated perfused gills of the crab Chasmagnathus granulatus. DA applied under steady state conditions (perfusion with hemolymph-like saline) produced a transient increase of the transepithelial potential difference (Vtc) from 2.2±0.2 to 4.8±0.3 mV, describing an initial cAMP-dependent stimulating phase followed by an inhibitory phase. Spiperone and domperidone (antagonists of D2-like DA receptors in vertebrates) completely blocked the response to DA, while the D1-like antagonist SCH23390 blocked only the inhibitory phase. Theophylline (phosphodiesterase inhibitor) and okadaic acid (protein phosphatases PP1 and PP2A inhibitor) were also able to block the inhibitory phase, suggesting that it depends on adenylyl cyclase inhibition and on protein phosphatases. When the gills were perfused with hypoosmotic solution, or with the adenylyl cyclase activator forskolin, Vte was increased several-fold. DA applied under these stimulated conditions partially reversed the Vte increase by 54% and 25%, respectively. Similarly, the D1-like agonist, fenoldopam, produced a 33% reduction in the stimulated Vte. We propose that, in C. granulatus gills, DA stimulates adenylyl cyclase and therefore ion transport through D1-like receptors linked to a Gs protein, although they respond to antagonists that interact with D2-like receptors in vertebrates. The inhibitory phase seems to be mediated by D2-like receptors linked to a Gi/o protein, which inhibits adenylyl cyclase, although these receptors can be activated or blocked by agonists or antagonists that interact with D1-like receptors in vertebrates and insects. Fil:Genovese, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ortiz, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Regueira, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tresguerres, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220949_v209_n14_p2785_Genovese |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
cAMP Chasmagnathus granulatus Crab Dopamine antagonist Dopamine receptor dopamine agonist Na +/K+-ATPase Okadaic acid Transepithelial potential differences adenosine triphosphatase (potassium sodium) cyclic AMP dependent protein kinase dopamine 1 receptor dopamine 2 receptor okadaic acid theophylline animal article Brachyura gill ion transport male metabolism osmolarity physiology signal transduction Animals Brachyura Cyclic AMP-Dependent Protein Kinases Gills Ion Transport Male Na(+)-K(+)-Exchanging ATPase Okadaic Acid Osmolar Concentration Receptors, Dopamine D1 Receptors, Dopamine D2 Signal Transduction Theophylline Chasmagnathus granulata Decapoda (Crustacea) Hexapoda Insecta Vertebrata |
| spellingShingle |
cAMP Chasmagnathus granulatus Crab Dopamine antagonist Dopamine receptor dopamine agonist Na +/K+-ATPase Okadaic acid Transepithelial potential differences adenosine triphosphatase (potassium sodium) cyclic AMP dependent protein kinase dopamine 1 receptor dopamine 2 receptor okadaic acid theophylline animal article Brachyura gill ion transport male metabolism osmolarity physiology signal transduction Animals Brachyura Cyclic AMP-Dependent Protein Kinases Gills Ion Transport Male Na(+)-K(+)-Exchanging ATPase Okadaic Acid Osmolar Concentration Receptors, Dopamine D1 Receptors, Dopamine D2 Signal Transduction Theophylline Chasmagnathus granulata Decapoda (Crustacea) Hexapoda Insecta Vertebrata Genovese, G. Senek, M. Ortiz, N. Regueira, M. Towle, D.W. Tresguerres, M. Luquet, C.M. Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors |
| topic_facet |
cAMP Chasmagnathus granulatus Crab Dopamine antagonist Dopamine receptor dopamine agonist Na +/K+-ATPase Okadaic acid Transepithelial potential differences adenosine triphosphatase (potassium sodium) cyclic AMP dependent protein kinase dopamine 1 receptor dopamine 2 receptor okadaic acid theophylline animal article Brachyura gill ion transport male metabolism osmolarity physiology signal transduction Animals Brachyura Cyclic AMP-Dependent Protein Kinases Gills Ion Transport Male Na(+)-K(+)-Exchanging ATPase Okadaic Acid Osmolar Concentration Receptors, Dopamine D1 Receptors, Dopamine D2 Signal Transduction Theophylline Chasmagnathus granulata Decapoda (Crustacea) Hexapoda Insecta Vertebrata |
| description |
The effects of dopamine (DA) and dopaminergic agonists and antagonists on ion transport were studied in isolated perfused gills of the crab Chasmagnathus granulatus. DA applied under steady state conditions (perfusion with hemolymph-like saline) produced a transient increase of the transepithelial potential difference (Vtc) from 2.2±0.2 to 4.8±0.3 mV, describing an initial cAMP-dependent stimulating phase followed by an inhibitory phase. Spiperone and domperidone (antagonists of D2-like DA receptors in vertebrates) completely blocked the response to DA, while the D1-like antagonist SCH23390 blocked only the inhibitory phase. Theophylline (phosphodiesterase inhibitor) and okadaic acid (protein phosphatases PP1 and PP2A inhibitor) were also able to block the inhibitory phase, suggesting that it depends on adenylyl cyclase inhibition and on protein phosphatases. When the gills were perfused with hypoosmotic solution, or with the adenylyl cyclase activator forskolin, Vte was increased several-fold. DA applied under these stimulated conditions partially reversed the Vte increase by 54% and 25%, respectively. Similarly, the D1-like agonist, fenoldopam, produced a 33% reduction in the stimulated Vte. We propose that, in C. granulatus gills, DA stimulates adenylyl cyclase and therefore ion transport through D1-like receptors linked to a Gs protein, although they respond to antagonists that interact with D2-like receptors in vertebrates. The inhibitory phase seems to be mediated by D2-like receptors linked to a Gi/o protein, which inhibits adenylyl cyclase, although these receptors can be activated or blocked by agonists or antagonists that interact with D1-like receptors in vertebrates and insects. |
| format |
Artículo Artículo publishedVersion |
| author |
Genovese, G. Senek, M. Ortiz, N. Regueira, M. Towle, D.W. Tresguerres, M. Luquet, C.M. |
| author_facet |
Genovese, G. Senek, M. Ortiz, N. Regueira, M. Towle, D.W. Tresguerres, M. Luquet, C.M. |
| author_sort |
Genovese, G. |
| title |
Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors |
| title_short |
Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors |
| title_full |
Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors |
| title_fullStr |
Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors |
| title_full_unstemmed |
Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors |
| title_sort |
dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab chasmagnathus granulatus: interaction between d1- and d2-like receptors |
| publishDate |
2006 |
| url |
http://hdl.handle.net/20.500.12110/paper_00220949_v209_n14_p2785_Genovese |
| work_keys_str_mv |
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