Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias
1. 1. Preliminary experiments with Euglena gracilis indicated that an endogenous factor which modified enzymic synthesis of porphyrinogens from PBG, was present in Homogenates (H) and Supernatant (S) fractions. 2. 2. When H or S was stored at 4-6°C, enzymic activity underwent an apparent spontaneous...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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1981
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0020711X_v13_n3_p343_DeGeralnik |
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paperaa:paper_0020711X_v13_n3_p343_DeGeralnik2023-06-12T16:42:10Z Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias Int. J. Biochem. 1981;13(3):343-353 De Geralnik, A.A.J. Rossetti, M.V. Del Carmen Batlle, A.M. ammonia lyase Ammonia Lyases folic acid glutathione porphyrin porphyrinogen adult article biosynthesis enzyme activation Euglena gracilis female gel chromatography heat human metabolism molecular weight porphyria Adult Ammonia-Lyases Chromatography, Gel Enzyme Activation Euglena gracilis Female Folic Acid Glutathione Heat Human Molecular Weight Porphyria Porphyrinogens Porphyrins Support, Non-U.S. Gov't 1. 1. Preliminary experiments with Euglena gracilis indicated that an endogenous factor which modified enzymic synthesis of porphyrinogens from PBG, was present in Homogenates (H) and Supernatant (S) fractions. 2. 2. When H or S was stored at 4-6°C, enzymic activity underwent an apparent spontaneous activation, increasing by as much as 7.5-8 times after 14 and 22 days of aging respectively. 3. 3. Experiments were carried out to detect, isolate and identify this factor. S and H were heated and the effect of the protein free supernatants (Hø,Sø) on activity were tested. By gel filtration of H and S, a low molecular weight compound (FH, FS) was separated, and the activity of the eluated protein (PrH, PrS) was enhanced 10-12 times. 4. 4. Addition of either Hø, Sø, FH or FS to different E. gracilis preparations increased their activities, suggesting the existence of a low molecular weight, heat-stable factor which would act stimulating enzymic synthesis of porphyrinogens. However some differences in the properties of the factor present in Hø or Sø and that present in FH or FS were observed. 5. 5. Studies on the FH and FS, confirmed that the factor was heat-stable, upon storage at 4°C its activation properties were not modified; but they were destroyed by basic or acid treatment. 6. 6. The same degree of activation as that produced by FH and FS on H, S, PrH and PrS, was obtained by replacing the factor solution by 10-7M folic acid or 10-3-10-2M Gluthation (GSH); however, neither the factor nor folic acid or GSH has any effect on the pellet enzyme, either bound to the membrane or solubilized by means of a chaothropic agent. 7. 7. The potential use of this factor in the treatment of acute porphyrias was indirectly investigated, by treating acute intermittent porphyria patients in early or acute attack with folic acid; after its oral administration at a dose of 30 mg daily for not longer than 10 days, both biochemical and clinical recovery followed. 8. 8. A scheme to explain the role of this factor in acting and controlling porphobilinogenase activity in E. gracilis is proposed. © 1981. Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Carmen Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1981 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0020711X_v13_n3_p343_DeGeralnik |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
ammonia lyase Ammonia Lyases folic acid glutathione porphyrin porphyrinogen adult article biosynthesis enzyme activation Euglena gracilis female gel chromatography heat human metabolism molecular weight porphyria Adult Ammonia-Lyases Chromatography, Gel Enzyme Activation Euglena gracilis Female Folic Acid Glutathione Heat Human Molecular Weight Porphyria Porphyrinogens Porphyrins Support, Non-U.S. Gov't |
| spellingShingle |
ammonia lyase Ammonia Lyases folic acid glutathione porphyrin porphyrinogen adult article biosynthesis enzyme activation Euglena gracilis female gel chromatography heat human metabolism molecular weight porphyria Adult Ammonia-Lyases Chromatography, Gel Enzyme Activation Euglena gracilis Female Folic Acid Glutathione Heat Human Molecular Weight Porphyria Porphyrinogens Porphyrins Support, Non-U.S. Gov't De Geralnik, A.A.J. Rossetti, M.V. Del Carmen Batlle, A.M. Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias |
| topic_facet |
ammonia lyase Ammonia Lyases folic acid glutathione porphyrin porphyrinogen adult article biosynthesis enzyme activation Euglena gracilis female gel chromatography heat human metabolism molecular weight porphyria Adult Ammonia-Lyases Chromatography, Gel Enzyme Activation Euglena gracilis Female Folic Acid Glutathione Heat Human Molecular Weight Porphyria Porphyrinogens Porphyrins Support, Non-U.S. Gov't |
| description |
1. 1. Preliminary experiments with Euglena gracilis indicated that an endogenous factor which modified enzymic synthesis of porphyrinogens from PBG, was present in Homogenates (H) and Supernatant (S) fractions. 2. 2. When H or S was stored at 4-6°C, enzymic activity underwent an apparent spontaneous activation, increasing by as much as 7.5-8 times after 14 and 22 days of aging respectively. 3. 3. Experiments were carried out to detect, isolate and identify this factor. S and H were heated and the effect of the protein free supernatants (Hø,Sø) on activity were tested. By gel filtration of H and S, a low molecular weight compound (FH, FS) was separated, and the activity of the eluated protein (PrH, PrS) was enhanced 10-12 times. 4. 4. Addition of either Hø, Sø, FH or FS to different E. gracilis preparations increased their activities, suggesting the existence of a low molecular weight, heat-stable factor which would act stimulating enzymic synthesis of porphyrinogens. However some differences in the properties of the factor present in Hø or Sø and that present in FH or FS were observed. 5. 5. Studies on the FH and FS, confirmed that the factor was heat-stable, upon storage at 4°C its activation properties were not modified; but they were destroyed by basic or acid treatment. 6. 6. The same degree of activation as that produced by FH and FS on H, S, PrH and PrS, was obtained by replacing the factor solution by 10-7M folic acid or 10-3-10-2M Gluthation (GSH); however, neither the factor nor folic acid or GSH has any effect on the pellet enzyme, either bound to the membrane or solubilized by means of a chaothropic agent. 7. 7. The potential use of this factor in the treatment of acute porphyrias was indirectly investigated, by treating acute intermittent porphyria patients in early or acute attack with folic acid; after its oral administration at a dose of 30 mg daily for not longer than 10 days, both biochemical and clinical recovery followed. 8. 8. A scheme to explain the role of this factor in acting and controlling porphobilinogenase activity in E. gracilis is proposed. © 1981. |
| format |
Artículo Artículo publishedVersion |
| author |
De Geralnik, A.A.J. Rossetti, M.V. Del Carmen Batlle, A.M. |
| author_facet |
De Geralnik, A.A.J. Rossetti, M.V. Del Carmen Batlle, A.M. |
| author_sort |
De Geralnik, A.A.J. |
| title |
Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias |
| title_short |
Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias |
| title_full |
Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias |
| title_fullStr |
Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias |
| title_full_unstemmed |
Porphyrin biosynthesis in Euglena gracilis-IV. An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias |
| title_sort |
porphyrin biosynthesis in euglena gracilis-iv. an endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias |
| publishDate |
1981 |
| url |
http://hdl.handle.net/20.500.12110/paper_0020711X_v13_n3_p343_DeGeralnik |
| work_keys_str_mv |
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| _version_ |
1769810077240262656 |