The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence

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Brucella spp., like other pathogens, must cope with the environment of diverse host niches during the infection process. In doing this, pathogens evolved different type of transport systems to help them survive and disseminate within the host. Members of the TolC family have been shown to be involve...

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Autores principales:	Posadas, D.M., Martín, F.A., Sabio Y Garcïa, J.V., Spera, J.M., Delpino, M.V., Baldi, P., Campos, E., Cravero, S.L., Zorreguieta, A.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2007
Materias:	acriflavine amikacin ampicillin antiinfective agent berberine bile salt carbenicillin cetrimide chloramphenicol deoxycholate sodium erythromycin ethidium bromide hemolysin nalidixic acid norfloxacin rhodamine rifampicin spectinomycin tetracycline TolC protein animal cell antibiotic resistance article bacterial gene bacterial mutation bacterial survival bacterial virulence BepC gene Brucella suis controlled study environmental factor Escherichia coli female gene disruption gene expression regulation gene insertion molecular cloning mouse nonhuman phenotype phylogeny priority journal protein analysis protein family Animals Anti-Infective Agents Bacterial Outer Membrane Proteins Cloning, Molecular Drug Resistance Female Membrane Transport Proteins Mice Mice, Inbred BALB C Phylogeny Polymerase Chain Reaction Virulence
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00199567_v75_n1_p379_Posadas
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_00199567_v75_n1_p379_Posadas
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spelling	paperaa:paper_00199567_v75_n1_p379_Posadas2023-06-12T16:42:06Z The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence Infect. Immun. 2007;75(1):379-389 Posadas, D.M. Martín, F.A. Sabio Y Garcïa, J.V. Spera, J.M. Delpino, M.V. Baldi, P. Campos, E. Cravero, S.L. Zorreguieta, A. acriflavine amikacin ampicillin antiinfective agent berberine bile salt carbenicillin cetrimide chloramphenicol deoxycholate sodium erythromycin ethidium bromide hemolysin nalidixic acid norfloxacin rhodamine rifampicin spectinomycin tetracycline TolC protein animal cell antibiotic resistance article bacterial gene bacterial mutation bacterial survival bacterial virulence BepC gene Brucella suis controlled study environmental factor Escherichia coli female gene disruption gene expression regulation gene insertion molecular cloning mouse nonhuman phenotype phylogeny priority journal protein analysis protein family Animals Anti-Infective Agents Bacterial Outer Membrane Proteins Brucella suis Cloning, Molecular Drug Resistance Female Membrane Transport Proteins Mice Mice, Inbred BALB C Phylogeny Polymerase Chain Reaction Virulence Brucella spp., like other pathogens, must cope with the environment of diverse host niches during the infection process. In doing this, pathogens evolved different type of transport systems to help them survive and disseminate within the host. Members of the TolC family have been shown to be involved in the export of chemically diverse molecules ranging from large protein toxins to small toxic compounds. The role of proteins from the TolC family in Brucella and other α-2-proteobacteria has been explored little. The gene encoding the unique member of the TolC family from Brucella suis (BepC) was cloned and expressed in an Escherichia coli mutant disrupted in the gene encoding TolC, which has the peculiarity of being involved in diverse transport functions. BepC fully complemented the resistance to drugs such as chloramphenicol and acriflavine but was incapable of restoring hemolysin secretion in the tolC mutant of & coli. An insertional mutation in the bepC gene strongly affected the resistance phenotype of B. suis to bile salts and toxic chemicals such as ethidium bromide and rhodamine and significantly decreased the resistance to antibiotics such as erythromycin, ampicillin, tetracycline, and norfloxacin. Moreover, the B. suis bepC mutant was attenuated in the mouse model of infection. Taken together, these results suggest that BepC-dependent efflux processes of toxic compounds contribute to B. suis survival inside the host. Copyright © 2007, American Society for Microbiology. All Rights Reserved. Fil:Posadas, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martín, F.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sabio Y Garcïa, J.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Campos, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zorreguieta, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00199567_v75_n1_p379_Posadas
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	acriflavine amikacin ampicillin antiinfective agent berberine bile salt carbenicillin cetrimide chloramphenicol deoxycholate sodium erythromycin ethidium bromide hemolysin nalidixic acid norfloxacin rhodamine rifampicin spectinomycin tetracycline TolC protein animal cell antibiotic resistance article bacterial gene bacterial mutation bacterial survival bacterial virulence BepC gene Brucella suis controlled study environmental factor Escherichia coli female gene disruption gene expression regulation gene insertion molecular cloning mouse nonhuman phenotype phylogeny priority journal protein analysis protein family Animals Anti-Infective Agents Bacterial Outer Membrane Proteins Brucella suis Cloning, Molecular Drug Resistance Female Membrane Transport Proteins Mice Mice, Inbred BALB C Phylogeny Polymerase Chain Reaction Virulence
spellingShingle	acriflavine amikacin ampicillin antiinfective agent berberine bile salt carbenicillin cetrimide chloramphenicol deoxycholate sodium erythromycin ethidium bromide hemolysin nalidixic acid norfloxacin rhodamine rifampicin spectinomycin tetracycline TolC protein animal cell antibiotic resistance article bacterial gene bacterial mutation bacterial survival bacterial virulence BepC gene Brucella suis controlled study environmental factor Escherichia coli female gene disruption gene expression regulation gene insertion molecular cloning mouse nonhuman phenotype phylogeny priority journal protein analysis protein family Animals Anti-Infective Agents Bacterial Outer Membrane Proteins Brucella suis Cloning, Molecular Drug Resistance Female Membrane Transport Proteins Mice Mice, Inbred BALB C Phylogeny Polymerase Chain Reaction Virulence Posadas, D.M. Martín, F.A. Sabio Y Garcïa, J.V. Spera, J.M. Delpino, M.V. Baldi, P. Campos, E. Cravero, S.L. Zorreguieta, A. The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence
topic_facet	acriflavine amikacin ampicillin antiinfective agent berberine bile salt carbenicillin cetrimide chloramphenicol deoxycholate sodium erythromycin ethidium bromide hemolysin nalidixic acid norfloxacin rhodamine rifampicin spectinomycin tetracycline TolC protein animal cell antibiotic resistance article bacterial gene bacterial mutation bacterial survival bacterial virulence BepC gene Brucella suis controlled study environmental factor Escherichia coli female gene disruption gene expression regulation gene insertion molecular cloning mouse nonhuman phenotype phylogeny priority journal protein analysis protein family Animals Anti-Infective Agents Bacterial Outer Membrane Proteins Brucella suis Cloning, Molecular Drug Resistance Female Membrane Transport Proteins Mice Mice, Inbred BALB C Phylogeny Polymerase Chain Reaction Virulence
description	Brucella spp., like other pathogens, must cope with the environment of diverse host niches during the infection process. In doing this, pathogens evolved different type of transport systems to help them survive and disseminate within the host. Members of the TolC family have been shown to be involved in the export of chemically diverse molecules ranging from large protein toxins to small toxic compounds. The role of proteins from the TolC family in Brucella and other α-2-proteobacteria has been explored little. The gene encoding the unique member of the TolC family from Brucella suis (BepC) was cloned and expressed in an Escherichia coli mutant disrupted in the gene encoding TolC, which has the peculiarity of being involved in diverse transport functions. BepC fully complemented the resistance to drugs such as chloramphenicol and acriflavine but was incapable of restoring hemolysin secretion in the tolC mutant of & coli. An insertional mutation in the bepC gene strongly affected the resistance phenotype of B. suis to bile salts and toxic chemicals such as ethidium bromide and rhodamine and significantly decreased the resistance to antibiotics such as erythromycin, ampicillin, tetracycline, and norfloxacin. Moreover, the B. suis bepC mutant was attenuated in the mouse model of infection. Taken together, these results suggest that BepC-dependent efflux processes of toxic compounds contribute to B. suis survival inside the host. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
format	Artículo Artículo publishedVersion
author	Posadas, D.M. Martín, F.A. Sabio Y Garcïa, J.V. Spera, J.M. Delpino, M.V. Baldi, P. Campos, E. Cravero, S.L. Zorreguieta, A.
author_facet	Posadas, D.M. Martín, F.A. Sabio Y Garcïa, J.V. Spera, J.M. Delpino, M.V. Baldi, P. Campos, E. Cravero, S.L. Zorreguieta, A.
author_sort	Posadas, D.M.
title	The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence
title_short	The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence
title_full	The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence
title_fullStr	The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence
title_full_unstemmed	The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence
title_sort	tolc homologue of brucella suis is involved in resistance to antimicrobial compounds and virulence
publishDate	2007
url	http://hdl.handle.net/20.500.12110/paper_00199567_v75_n1_p379_Posadas
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The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence

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