Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi

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The lipopeptidophosphoglycan from Trypanosoma cruzi is a glycosylated inositol‐phosphoceramide isolated from epimastigotes at the stationary phase of growth (4–5 days). We have now purified two similar glycoinositolphospholipids (glycoinositolphospholipid A and glycoinositolphospholipid B) from epim...

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Autores principales: De Lederkremer, R.M., Lima, C.E., Ramirez, M.I., Goncalvez, M.F., Colli, W.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 1993
Materias:
ceramide
glycoinositolphospholipid
glycophospoinositol
phospholipase c
unclassified drug
article
epimastigote
nonhuman
priority journal
trypanosoma cruzi
Animal
Carbohydrate Sequence
Ceramides
Chromatography, Gas
Glyceryl Ethers
Glycosylation
Glycosylphosphatidylinositols
Lipids
Methylation
Molecular Sequence Data
Palmitates
Polysaccharides
Spectrum Analysis, Mass
Support, Non-U.S. Gov't
Trypanosoma cruzi
Bacillus thuringiensis
Trypanosoma
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00142956_v218_n3_p929_DeLederkremer
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_00142956_v218_n3_p929_DeLederkremer
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spelling paperaa:paper_00142956_v218_n3_p929_DeLederkremer2023-06-12T16:41:41Z Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi Eur. J. Biochem. 1993;218(3):929-936 De Lederkremer, R.M. Lima, C.E. Ramirez, M.I. Goncalvez, M.F. Colli, W. ceramide glycoinositolphospholipid glycophospoinositol phospholipase c unclassified drug article epimastigote nonhuman priority journal trypanosoma cruzi Animal Carbohydrate Sequence Ceramides Chromatography, Gas Glyceryl Ethers Glycosylation Glycosylphosphatidylinositols Lipids Methylation Molecular Sequence Data Palmitates Polysaccharides Spectrum Analysis, Mass Support, Non-U.S. Gov't Trypanosoma cruzi Bacillus thuringiensis Trypanosoma Trypanosoma cruzi The lipopeptidophosphoglycan from Trypanosoma cruzi is a glycosylated inositol‐phosphoceramide isolated from epimastigotes at the stationary phase of growth (4–5 days). We have now purified two similar glycoinositolphospholipids (glycoinositolphospholipid A and glycoinositolphospholipid B) from epimastigotes after the second day of culture growth. [3H]Palmitic acid was incorporated into 1‐O‐hexadecyl‐2‐O‐palmitoylglycerol in glycoinositolphospholipid A and into ceramide in glycoinositolphospholipid B. The lipids were released by incubation with glycosylphosphatidylinositol‐specific phospholipase C from Bacillus thuringiensis or by chemical methods. After alkaline hydrolysis, the lipids were analysed by GLC/MS. In glycoinositolphospholipid A the resulting lipids corresponded to 1‐O‐hexadecylglycerol and palmitic acid. The ceramide components in glycoinositolphospholipid B are sphinganine, palmitic acid and lignoceric acid. The oligosaccharides could be degraded by nitrous acid and further enzymic treatment showed that the two glycoinositolphospholipids isolated from T. cruzi share the common core structure of the glycosylphosphatidylinositol membrane anchors. The microheterogeneity was determined, as well as the substitution by galactose, and was mainly in the furanose configuration as was previously described for lipopeptidophosphoglycan. However, methylation analysis indicated that 20% of the galactose is in the pyranose from. Both glycoinositolphospholipids mainly differ in the lipid moiety. Copyright © 1993, Wiley Blackwell. All rights reserved Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramirez, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1993 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142956_v218_n3_p929_DeLederkremer
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic ceramide
glycoinositolphospholipid
glycophospoinositol
phospholipase c
unclassified drug
article
epimastigote
nonhuman
priority journal
trypanosoma cruzi
Animal
Carbohydrate Sequence
Ceramides
Chromatography, Gas
Glyceryl Ethers
Glycosylation
Glycosylphosphatidylinositols
Lipids
Methylation
Molecular Sequence Data
Palmitates
Polysaccharides
Spectrum Analysis, Mass
Support, Non-U.S. Gov't
Trypanosoma cruzi
Bacillus thuringiensis
Trypanosoma
Trypanosoma cruzi
spellingShingle ceramide
glycoinositolphospholipid
glycophospoinositol
phospholipase c
unclassified drug
article
epimastigote
nonhuman
priority journal
trypanosoma cruzi
Animal
Carbohydrate Sequence
Ceramides
Chromatography, Gas
Glyceryl Ethers
Glycosylation
Glycosylphosphatidylinositols
Lipids
Methylation
Molecular Sequence Data
Palmitates
Polysaccharides
Spectrum Analysis, Mass
Support, Non-U.S. Gov't
Trypanosoma cruzi
Bacillus thuringiensis
Trypanosoma
Trypanosoma cruzi
De Lederkremer, R.M.
Lima, C.E.
Ramirez, M.I.
Goncalvez, M.F.
Colli, W.
Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi
topic_facet ceramide
glycoinositolphospholipid
glycophospoinositol
phospholipase c
unclassified drug
article
epimastigote
nonhuman
priority journal
trypanosoma cruzi
Animal
Carbohydrate Sequence
Ceramides
Chromatography, Gas
Glyceryl Ethers
Glycosylation
Glycosylphosphatidylinositols
Lipids
Methylation
Molecular Sequence Data
Palmitates
Polysaccharides
Spectrum Analysis, Mass
Support, Non-U.S. Gov't
Trypanosoma cruzi
Bacillus thuringiensis
Trypanosoma
Trypanosoma cruzi
description The lipopeptidophosphoglycan from Trypanosoma cruzi is a glycosylated inositol‐phosphoceramide isolated from epimastigotes at the stationary phase of growth (4–5 days). We have now purified two similar glycoinositolphospholipids (glycoinositolphospholipid A and glycoinositolphospholipid B) from epimastigotes after the second day of culture growth. [3H]Palmitic acid was incorporated into 1‐O‐hexadecyl‐2‐O‐palmitoylglycerol in glycoinositolphospholipid A and into ceramide in glycoinositolphospholipid B. The lipids were released by incubation with glycosylphosphatidylinositol‐specific phospholipase C from Bacillus thuringiensis or by chemical methods. After alkaline hydrolysis, the lipids were analysed by GLC/MS. In glycoinositolphospholipid A the resulting lipids corresponded to 1‐O‐hexadecylglycerol and palmitic acid. The ceramide components in glycoinositolphospholipid B are sphinganine, palmitic acid and lignoceric acid. The oligosaccharides could be degraded by nitrous acid and further enzymic treatment showed that the two glycoinositolphospholipids isolated from T. cruzi share the common core structure of the glycosylphosphatidylinositol membrane anchors. The microheterogeneity was determined, as well as the substitution by galactose, and was mainly in the furanose configuration as was previously described for lipopeptidophosphoglycan. However, methylation analysis indicated that 20% of the galactose is in the pyranose from. Both glycoinositolphospholipids mainly differ in the lipid moiety. Copyright © 1993, Wiley Blackwell. All rights reserved
format Artículo
Artículo
publishedVersion
author De Lederkremer, R.M.
Lima, C.E.
Ramirez, M.I.
Goncalvez, M.F.
Colli, W.
author_facet De Lederkremer, R.M.
Lima, C.E.
Ramirez, M.I.
Goncalvez, M.F.
Colli, W.
author_sort De Lederkremer, R.M.
title Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi
title_short Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi
title_full Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi
title_fullStr Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi
title_full_unstemmed Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi
title_sort hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in trypanosoma cruzi
publishDate 1993
url http://hdl.handle.net/20.500.12110/paper_00142956_v218_n3_p929_DeLederkremer
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AT limace hexadecylpalmitoyglycerolorceramideislinkedtosimilarglycophosphoinositolanchorlikestructuresintrypanosomacruzi
AT ramirezmi hexadecylpalmitoyglycerolorceramideislinkedtosimilarglycophosphoinositolanchorlikestructuresintrypanosomacruzi
AT goncalvezmf hexadecylpalmitoyglycerolorceramideislinkedtosimilarglycophosphoinositolanchorlikestructuresintrypanosomacruzi
AT colliw hexadecylpalmitoyglycerolorceramideislinkedtosimilarglycophosphoinositolanchorlikestructuresintrypanosomacruzi
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