Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)

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Mammalian fertilization is a complex multi-step process mediated by different molecules present on both gametes. Epididymal protein CRISP1, a member of the Cysteine-RIch Secretory Protein (CRISP) family, was identified by our laboratory and postulated to participate in both sperm-zona pellucida (ZP)...

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Autores principales:	Da Ros, V.G., Maldera, J.A., Willis, W.D., Cohen, D.J., Goulding, E.H., Gelman, D.M., Rubinstein, M., Eddy, E.M., Cuasnicu, P.S.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2008
Materias:	CRISP Egg Fertilization Sperm cysteine rich secretory protein 1 DNA RNA secretory protein tyrosine acrosome reaction animal cell animal experiment animal tissue article controlled study female fertility fertilization fertilization in vitro gene disruption genotype immunoblotting male mouse mutant nonhuman priority journal protein phosphorylation reverse transcription polymerase chain reaction sperm spermatozoon capacitation spermatozoon motility zona pellucida Animalia Mammalia Mus
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00121606_v320_n1_p12_DaRos
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_00121606_v320_n1_p12_DaRos
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spelling	paperaa:paper_00121606_v320_n1_p12_DaRos2023-06-12T16:41:34Z Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1) Dev. Biol. 2008;320(1):12-18 Da Ros, V.G. Maldera, J.A. Willis, W.D. Cohen, D.J. Goulding, E.H. Gelman, D.M. Rubinstein, M. Eddy, E.M. Cuasnicu, P.S. CRISP Egg Fertilization Sperm cysteine rich secretory protein 1 DNA RNA secretory protein tyrosine acrosome reaction animal cell animal experiment animal tissue article controlled study female fertility fertilization fertilization in vitro gene disruption genotype immunoblotting male mouse mutant nonhuman priority journal protein phosphorylation reverse transcription polymerase chain reaction sperm spermatozoon capacitation spermatozoon motility zona pellucida Animalia Mammalia Mus Mammalian fertilization is a complex multi-step process mediated by different molecules present on both gametes. Epididymal protein CRISP1, a member of the Cysteine-RIch Secretory Protein (CRISP) family, was identified by our laboratory and postulated to participate in both sperm-zona pellucida (ZP) interaction and gamete fusion by binding to egg-complementary sites. To elucidate the functional role of CRISP1 in vivo, we disrupted the Crisp1 gene and evaluated the effect on animal fertility and several sperm parameters. Male and female Crisp1-/- animals exhibited no differences in fertility compared to controls. Sperm motility and the ability to undergo a spontaneous or progesterone-induced acrosome reaction were neither affected in Crisp1-/- mice. However, the level of protein tyrosine phosphorylation during capacitation was clearly lower in mutant sperm than in controls. In vitro fertilization assays showed that Crisp1-/- sperm also exhibited a significantly reduced ability to penetrate both ZP-intact and ZP-free eggs. Moreover, when ZP-free eggs were simultaneously inseminated with Crisp1+/+ and Crisp1-/- sperm in a competition assay, the mutant sperm exhibited a greater disadvantage in their fusion ability. Finally, the finding that the fusion ability of Crisp1-/- sperm was further inhibited by the presence of CRISP1 or CRISP2 during gamete co-incubation, supports that another CRISP cooperates with CRISP1 during fertilization and might compensate for its lack in the mutant mice. Together, these results indicate that CRISP proteins are players in the mammalian fertilization process. To our knowledge this is the first knockout mice generated for a CRISP protein. The information obtained might have important functional implications for other members of the widely distributed and evolutionarily conserved CRISP family. © 2008 Elsevier Inc. Fil:Da Ros, V.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cohen, D.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gelman, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rubinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cuasnicu, P.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00121606_v320_n1_p12_DaRos
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	CRISP Egg Fertilization Sperm cysteine rich secretory protein 1 DNA RNA secretory protein tyrosine acrosome reaction animal cell animal experiment animal tissue article controlled study female fertility fertilization fertilization in vitro gene disruption genotype immunoblotting male mouse mutant nonhuman priority journal protein phosphorylation reverse transcription polymerase chain reaction sperm spermatozoon capacitation spermatozoon motility zona pellucida Animalia Mammalia Mus
spellingShingle	CRISP Egg Fertilization Sperm cysteine rich secretory protein 1 DNA RNA secretory protein tyrosine acrosome reaction animal cell animal experiment animal tissue article controlled study female fertility fertilization fertilization in vitro gene disruption genotype immunoblotting male mouse mutant nonhuman priority journal protein phosphorylation reverse transcription polymerase chain reaction sperm spermatozoon capacitation spermatozoon motility zona pellucida Animalia Mammalia Mus Da Ros, V.G. Maldera, J.A. Willis, W.D. Cohen, D.J. Goulding, E.H. Gelman, D.M. Rubinstein, M. Eddy, E.M. Cuasnicu, P.S. Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)
topic_facet	CRISP Egg Fertilization Sperm cysteine rich secretory protein 1 DNA RNA secretory protein tyrosine acrosome reaction animal cell animal experiment animal tissue article controlled study female fertility fertilization fertilization in vitro gene disruption genotype immunoblotting male mouse mutant nonhuman priority journal protein phosphorylation reverse transcription polymerase chain reaction sperm spermatozoon capacitation spermatozoon motility zona pellucida Animalia Mammalia Mus
description	Mammalian fertilization is a complex multi-step process mediated by different molecules present on both gametes. Epididymal protein CRISP1, a member of the Cysteine-RIch Secretory Protein (CRISP) family, was identified by our laboratory and postulated to participate in both sperm-zona pellucida (ZP) interaction and gamete fusion by binding to egg-complementary sites. To elucidate the functional role of CRISP1 in vivo, we disrupted the Crisp1 gene and evaluated the effect on animal fertility and several sperm parameters. Male and female Crisp1-/- animals exhibited no differences in fertility compared to controls. Sperm motility and the ability to undergo a spontaneous or progesterone-induced acrosome reaction were neither affected in Crisp1-/- mice. However, the level of protein tyrosine phosphorylation during capacitation was clearly lower in mutant sperm than in controls. In vitro fertilization assays showed that Crisp1-/- sperm also exhibited a significantly reduced ability to penetrate both ZP-intact and ZP-free eggs. Moreover, when ZP-free eggs were simultaneously inseminated with Crisp1+/+ and Crisp1-/- sperm in a competition assay, the mutant sperm exhibited a greater disadvantage in their fusion ability. Finally, the finding that the fusion ability of Crisp1-/- sperm was further inhibited by the presence of CRISP1 or CRISP2 during gamete co-incubation, supports that another CRISP cooperates with CRISP1 during fertilization and might compensate for its lack in the mutant mice. Together, these results indicate that CRISP proteins are players in the mammalian fertilization process. To our knowledge this is the first knockout mice generated for a CRISP protein. The information obtained might have important functional implications for other members of the widely distributed and evolutionarily conserved CRISP family. © 2008 Elsevier Inc.
format	Artículo Artículo publishedVersion
author	Da Ros, V.G. Maldera, J.A. Willis, W.D. Cohen, D.J. Goulding, E.H. Gelman, D.M. Rubinstein, M. Eddy, E.M. Cuasnicu, P.S.
author_facet	Da Ros, V.G. Maldera, J.A. Willis, W.D. Cohen, D.J. Goulding, E.H. Gelman, D.M. Rubinstein, M. Eddy, E.M. Cuasnicu, P.S.
author_sort	Da Ros, V.G.
title	Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)
title_short	Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)
title_full	Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)
title_fullStr	Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)
title_full_unstemmed	Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)
title_sort	impaired sperm fertilizing ability in mice lacking cysteine-rich secretory protein 1 (crisp1)
publishDate	2008
url	http://hdl.handle.net/20.500.12110/paper_00121606_v320_n1_p12_DaRos
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Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1)

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