Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores

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The organization of the cytoplasm is regulated by molecular motors, which transport organelles and other cargoes along cytoskeleton tracks. In this work, we use single particle tracking to study the in vivo regulation of the transport driven by myosin-V along actin filaments in Xenopus laevis melano...

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Detalles Bibliográficos
Autores principales: Brunstein, M., Bruno, L., Desposito, M., Levi, V.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2009
Materias:
actin
molecular motor
myosin V
actin filament
active transport
animal cell
article
cell organelle
controlled study
diffusion
melanophore
melanosome
microtubule assembly
nonhuman
prediction
transport diffusion model
Xenopus laevis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00063495_v97_n6_p1548_Brunstein
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_00063495_v97_n6_p1548_Brunstein
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spelling paperaa:paper_00063495_v97_n6_p1548_Brunstein2023-06-12T16:41:20Z Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores Biophys. J. 2009;97(6):1548-1557 Brunstein, M. Bruno, L. Desposito, M. Levi, V. actin molecular motor myosin V actin filament active transport animal cell article cell organelle controlled study diffusion melanophore melanosome microtubule assembly nonhuman prediction transport diffusion model Xenopus laevis Xenopus laevis The organization of the cytoplasm is regulated by molecular motors, which transport organelles and other cargoes along cytoskeleton tracks. In this work, we use single particle tracking to study the in vivo regulation of the transport driven by myosin-V along actin filaments in Xenopus laevis melanophores. Melanophores have pigment organelles or melanosomes, which, in response to hormones, disperse in the cytoplasm or aggregate in the perinuclear region. We followed the motion of melanosomes in cells treated to depolymerize microtubules during aggregation and dispersion, focusing the analysis on the dynamics of these organelles in a time window not explored before to our knowledge. These data could not be explained by previous models that only consider active transport. We proposed a transport-diffusion model in which melanosomes may detach from actin tracks and reattach to nearby filaments to resume the active motion after a given time of diffusion. This model predicts that organelles spend -70% and 10% of the total time in active transport during dispersion and aggregation, respectively. Our results suggest that the transport along actin filaments and the switching from actin to microtubule networks are regulated by changes in the diffusion time between periods of active motion driven by myosin-V. © 2009 by the Biophysical Society. Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Desposito, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00063495_v97_n6_p1548_Brunstein
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic actin
molecular motor
myosin V
actin filament
active transport
animal cell
article
cell organelle
controlled study
diffusion
melanophore
melanosome
microtubule assembly
nonhuman
prediction
transport diffusion model
Xenopus laevis
Xenopus laevis
spellingShingle actin
molecular motor
myosin V
actin filament
active transport
animal cell
article
cell organelle
controlled study
diffusion
melanophore
melanosome
microtubule assembly
nonhuman
prediction
transport diffusion model
Xenopus laevis
Xenopus laevis
Brunstein, M.
Bruno, L.
Desposito, M.
Levi, V.
Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores
topic_facet actin
molecular motor
myosin V
actin filament
active transport
animal cell
article
cell organelle
controlled study
diffusion
melanophore
melanosome
microtubule assembly
nonhuman
prediction
transport diffusion model
Xenopus laevis
Xenopus laevis
description The organization of the cytoplasm is regulated by molecular motors, which transport organelles and other cargoes along cytoskeleton tracks. In this work, we use single particle tracking to study the in vivo regulation of the transport driven by myosin-V along actin filaments in Xenopus laevis melanophores. Melanophores have pigment organelles or melanosomes, which, in response to hormones, disperse in the cytoplasm or aggregate in the perinuclear region. We followed the motion of melanosomes in cells treated to depolymerize microtubules during aggregation and dispersion, focusing the analysis on the dynamics of these organelles in a time window not explored before to our knowledge. These data could not be explained by previous models that only consider active transport. We proposed a transport-diffusion model in which melanosomes may detach from actin tracks and reattach to nearby filaments to resume the active motion after a given time of diffusion. This model predicts that organelles spend -70% and 10% of the total time in active transport during dispersion and aggregation, respectively. Our results suggest that the transport along actin filaments and the switching from actin to microtubule networks are regulated by changes in the diffusion time between periods of active motion driven by myosin-V. © 2009 by the Biophysical Society.
format Artículo
Artículo
publishedVersion
author Brunstein, M.
Bruno, L.
Desposito, M.
Levi, V.
author_facet Brunstein, M.
Bruno, L.
Desposito, M.
Levi, V.
author_sort Brunstein, M.
title Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores
title_short Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores
title_full Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores
title_fullStr Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores
title_full_unstemmed Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores
title_sort anomalous dynamics of melanosomes driven by myosin-v in xenopus laevis melanophores
publishDate 2009
url http://hdl.handle.net/20.500.12110/paper_00063495_v97_n6_p1548_Brunstein
work_keys_str_mv AT brunsteinm anomalousdynamicsofmelanosomesdrivenbymyosinvinxenopuslaevismelanophores
AT brunol anomalousdynamicsofmelanosomesdrivenbymyosinvinxenopuslaevismelanophores
AT despositom anomalousdynamicsofmelanosomesdrivenbymyosinvinxenopuslaevismelanophores
AT leviv anomalousdynamicsofmelanosomesdrivenbymyosinvinxenopuslaevismelanophores
_version_ 1769810154530799616

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