δ-aminolaevulinate synthetase in extracts of cultured soybean cells

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1. 1. δ-Aminolaevulinate synthetase has been detected in extracts of soybean callus tissues and the enzyme activity reached its maximum when callus were 11 days old. 2. 2. The presence of a compound which seems to control δ-aminolaevulinate synthetase activity was demonstrated. The enzyme was presen...

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Autores principales: De Xifra, E.A.W., Del C. Batlle, A.M., Tigier, H.A.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 1971
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00052744_v235_n3_p511_DeXifra
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_00052744_v235_n3_p511_DeXifra
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spelling paperaa:paper_00052744_v235_n3_p511_DeXifra2023-06-12T16:41:15Z δ-aminolaevulinate synthetase in extracts of cultured soybean cells BBA - Enzymology 1971;235(3):511-517 De Xifra, E.A.W. Del C. Batlle, A.M. Tigier, H.A. 1. 1. δ-Aminolaevulinate synthetase has been detected in extracts of soybean callus tissues and the enzyme activity reached its maximum when callus were 11 days old. 2. 2. The presence of a compound which seems to control δ-aminolaevulinate synthetase activity was demonstrated. The enzyme was present in the soluble fraction and was very labile. 3. 3. When crude extracts or 500 × g supernatant were stored at 4-6°, the apparent activity of δ-aminolaevulinate synthetase increased by as much as 3-6 times, while the activities of δ-aminolaevulinate dehydratase and succinyl-CoA synthetase did not significantly change during the storage. Activation was dependent on concentrations of cells suspensions during disruption and aging. 4. 4. Gel filtration with Sephadex G-25 of 2000 × g supernatants produced an enzyme fraction 30% more active. An increase in enzyme activity was observed when dark-grown callus were exposed to light. 5. 5. The addition of ATP, gibberellic acid and δ-aminolaevulinate to the culture media diminished activity; iron deficiency also produced an δ-aminolaevulinate synthetase less active. © 1971. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tigier, H.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1971 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00052744_v235_n3_p511_DeXifra
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
description 1. 1. δ-Aminolaevulinate synthetase has been detected in extracts of soybean callus tissues and the enzyme activity reached its maximum when callus were 11 days old. 2. 2. The presence of a compound which seems to control δ-aminolaevulinate synthetase activity was demonstrated. The enzyme was present in the soluble fraction and was very labile. 3. 3. When crude extracts or 500 × g supernatant were stored at 4-6°, the apparent activity of δ-aminolaevulinate synthetase increased by as much as 3-6 times, while the activities of δ-aminolaevulinate dehydratase and succinyl-CoA synthetase did not significantly change during the storage. Activation was dependent on concentrations of cells suspensions during disruption and aging. 4. 4. Gel filtration with Sephadex G-25 of 2000 × g supernatants produced an enzyme fraction 30% more active. An increase in enzyme activity was observed when dark-grown callus were exposed to light. 5. 5. The addition of ATP, gibberellic acid and δ-aminolaevulinate to the culture media diminished activity; iron deficiency also produced an δ-aminolaevulinate synthetase less active. © 1971.
format Artículo
Artículo
publishedVersion
author De Xifra, E.A.W.
Del C. Batlle, A.M.
Tigier, H.A.
spellingShingle De Xifra, E.A.W.
Del C. Batlle, A.M.
Tigier, H.A.
δ-aminolaevulinate synthetase in extracts of cultured soybean cells
author_facet De Xifra, E.A.W.
Del C. Batlle, A.M.
Tigier, H.A.
author_sort De Xifra, E.A.W.
title δ-aminolaevulinate synthetase in extracts of cultured soybean cells
title_short δ-aminolaevulinate synthetase in extracts of cultured soybean cells
title_full δ-aminolaevulinate synthetase in extracts of cultured soybean cells
title_fullStr δ-aminolaevulinate synthetase in extracts of cultured soybean cells
title_full_unstemmed δ-aminolaevulinate synthetase in extracts of cultured soybean cells
title_sort δ-aminolaevulinate synthetase in extracts of cultured soybean cells
publishDate 1971
url http://hdl.handle.net/20.500.12110/paper_00052744_v235_n3_p511_DeXifra
work_keys_str_mv AT dexifraeaw daminolaevulinatesynthetaseinextractsofculturedsoybeancells
AT delcbatlleam daminolaevulinatesynthetaseinextractsofculturedsoybeancells
AT tigierha daminolaevulinatesynthetaseinextractsofculturedsoybeancells
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