Fructokinase from rat liver. I. Purification and properties

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Fructokinase (ATP:d-fructose-1-phosphate transferase, EC 2.7.1.3) from rat liver has been purified 400-fold. The purification procedure involves an acid treatment, a heat step at 65°, (NH4)2SO4 fractionation, chromatography on Sephadex G-100 and finally (NH4)2SO4 extraction. The enzyme appears nearl...

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Autores principales:	Sánchez, J.J., González, N.S., Pontis, H.G.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	1971
Materias:	4 chloromercuribenzoic acid acrylic acid derivative adenine nucleotide ammonium derivative absorption animal article biosynthesis colorimetry density gradient centrifugation Absorption Acrylates Adenine Nucleotides Ammonium Compounds Animal Centrifugation, Density Gradient Chloromercuribenzoates Colorimetry
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00052744_v227_n1_p67_Sanchez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_00052744_v227_n1_p67_Sanchez
record_format	dspace
spelling	paperaa:paper_00052744_v227_n1_p67_Sanchez2023-06-12T16:41:14Z Fructokinase from rat liver. I. Purification and properties BBA - Enzymology 1971;227(1):67-78 Sánchez, J.J. González, N.S. Pontis, H.G. 4 chloromercuribenzoic acid acrylic acid derivative adenine nucleotide ammonium derivative absorption animal article biosynthesis colorimetry density gradient centrifugation Absorption Acrylates Adenine Nucleotides Ammonium Compounds Animal Centrifugation, Density Gradient Chloromercuribenzoates Colorimetry Fructokinase (ATP:d-fructose-1-phosphate transferase, EC 2.7.1.3) from rat liver has been purified 400-fold. The purification procedure involves an acid treatment, a heat step at 65°, (NH4)2SO4 fractionation, chromatography on Sephadex G-100 and finally (NH4)2SO4 extraction. The enzyme appears nearly homogenous by density gradient centrifugation but gives a single peak in sedimentation velocity analysis. Purified liver fructokinase has a Km of 0.46-0.80 mM for fructose and 1.56-1.33 mM for MgATP at a K+ concentration of 0.4 and 0.1 M, respectively. The enzyme also phosphorylates l-sorbose and d-tagatose. No difference could be found in the phosphorylation of the pyranose and furanose forms of fructose. The enzyme is inhibited by p-chloromercuribenzoate and is stable up to 50-55°. © 1971. Fil:González, N.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pontis, H.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1971 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00052744_v227_n1_p67_Sanchez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	4 chloromercuribenzoic acid acrylic acid derivative adenine nucleotide ammonium derivative absorption animal article biosynthesis colorimetry density gradient centrifugation Absorption Acrylates Adenine Nucleotides Ammonium Compounds Animal Centrifugation, Density Gradient Chloromercuribenzoates Colorimetry
spellingShingle	4 chloromercuribenzoic acid acrylic acid derivative adenine nucleotide ammonium derivative absorption animal article biosynthesis colorimetry density gradient centrifugation Absorption Acrylates Adenine Nucleotides Ammonium Compounds Animal Centrifugation, Density Gradient Chloromercuribenzoates Colorimetry Sánchez, J.J. González, N.S. Pontis, H.G. Fructokinase from rat liver. I. Purification and properties
topic_facet	4 chloromercuribenzoic acid acrylic acid derivative adenine nucleotide ammonium derivative absorption animal article biosynthesis colorimetry density gradient centrifugation Absorption Acrylates Adenine Nucleotides Ammonium Compounds Animal Centrifugation, Density Gradient Chloromercuribenzoates Colorimetry
description	Fructokinase (ATP:d-fructose-1-phosphate transferase, EC 2.7.1.3) from rat liver has been purified 400-fold. The purification procedure involves an acid treatment, a heat step at 65°, (NH4)2SO4 fractionation, chromatography on Sephadex G-100 and finally (NH4)2SO4 extraction. The enzyme appears nearly homogenous by density gradient centrifugation but gives a single peak in sedimentation velocity analysis. Purified liver fructokinase has a Km of 0.46-0.80 mM for fructose and 1.56-1.33 mM for MgATP at a K+ concentration of 0.4 and 0.1 M, respectively. The enzyme also phosphorylates l-sorbose and d-tagatose. No difference could be found in the phosphorylation of the pyranose and furanose forms of fructose. The enzyme is inhibited by p-chloromercuribenzoate and is stable up to 50-55°. © 1971.
format	Artículo Artículo publishedVersion
author	Sánchez, J.J. González, N.S. Pontis, H.G.
author_facet	Sánchez, J.J. González, N.S. Pontis, H.G.
author_sort	Sánchez, J.J.
title	Fructokinase from rat liver. I. Purification and properties
title_short	Fructokinase from rat liver. I. Purification and properties
title_full	Fructokinase from rat liver. I. Purification and properties
title_fullStr	Fructokinase from rat liver. I. Purification and properties
title_full_unstemmed	Fructokinase from rat liver. I. Purification and properties
title_sort	fructokinase from rat liver. i. purification and properties
publishDate	1971
url	http://hdl.handle.net/20.500.12110/paper_00052744_v227_n1_p67_Sanchez
work_keys_str_mv	AT sanchezjj fructokinasefromratliveripurificationandproperties AT gonzalezns fructokinasefromratliveripurificationandproperties AT pontishg fructokinasefromratliveripurificationandproperties
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Fructokinase from rat liver. I. Purification and properties

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