High affinity binding of melatonin in bovine medial basal hypothalamus

Specific binding was linear up to 3 mg of protein in the incubation tube. Melatonin binding was pH-dependent the maximum being observed at pH 7.4. By adding 6 mM Ca++ to the buffer a 2-fold increase of specific binding was observed. Scatchard analysis revealed a population of high affinity binding s...

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Guardado en:
Detalles Bibliográficos
Publicado: 1978
Materias:
melatonin
animal experiment
cattle
central nervous system
endocrine system
hypothalamus
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18622_v6_n9_p357_Cardinali
http://hdl.handle.net/20.500.12110/paper_NIS18622_v6_n9_p357_Cardinali
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_NIS18622_v6_n9_p357_Cardinali
record_format dspace
spelling paper:paper_NIS18622_v6_n9_p357_Cardinali2023-06-08T16:39:48Z High affinity binding of melatonin in bovine medial basal hypothalamus melatonin animal experiment cattle central nervous system endocrine system hypothalamus Specific binding was linear up to 3 mg of protein in the incubation tube. Melatonin binding was pH-dependent the maximum being observed at pH 7.4. By adding 6 mM Ca++ to the buffer a 2-fold increase of specific binding was observed. Scatchard analysis revealed a population of high affinity binding sites with a Kd=(1.2±0.4)x10-8 M in 3 successive experiments. 1978 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18622_v6_n9_p357_Cardinali http://hdl.handle.net/20.500.12110/paper_NIS18622_v6_n9_p357_Cardinali
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic melatonin
animal experiment
cattle
central nervous system
endocrine system
hypothalamus
spellingShingle melatonin
animal experiment
cattle
central nervous system
endocrine system
hypothalamus
High affinity binding of melatonin in bovine medial basal hypothalamus
topic_facet melatonin
animal experiment
cattle
central nervous system
endocrine system
hypothalamus
description Specific binding was linear up to 3 mg of protein in the incubation tube. Melatonin binding was pH-dependent the maximum being observed at pH 7.4. By adding 6 mM Ca++ to the buffer a 2-fold increase of specific binding was observed. Scatchard analysis revealed a population of high affinity binding sites with a Kd=(1.2±0.4)x10-8 M in 3 successive experiments.
title High affinity binding of melatonin in bovine medial basal hypothalamus
title_short High affinity binding of melatonin in bovine medial basal hypothalamus
title_full High affinity binding of melatonin in bovine medial basal hypothalamus
title_fullStr High affinity binding of melatonin in bovine medial basal hypothalamus
title_full_unstemmed High affinity binding of melatonin in bovine medial basal hypothalamus
title_sort high affinity binding of melatonin in bovine medial basal hypothalamus
publishDate 1978
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18622_v6_n9_p357_Cardinali
http://hdl.handle.net/20.500.12110/paper_NIS18622_v6_n9_p357_Cardinali
_version_ 1769175827195363328

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