Inorganic hydrogels for whole-culture encapsulation

Sol-gel encapsulation of living cells within inorganic hydrogels, mainly silica, is a promising technology for the design of biosensors. These host-guest functional materials maintain specific biologic functions of their guest while the properties of the host can be tuned to fulfill the requirements...

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Detalles Bibliográficos
Autores principales: Perullini, Ana Mercedes, Spedalieri, Ana Cecilia
Publicado: 2015
Materias:
Biosensors
Ecotoxicity
Sol-gel
Two-step procedure
Whole-culture encapsulation
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816346_v_n_p57_Perullini
http://hdl.handle.net/20.500.12110/paper_97816346_v_n_p57_Perullini
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_97816346_v_n_p57_Perullini
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spelling paper:paper_97816346_v_n_p57_Perullini2023-06-08T16:38:33Z Inorganic hydrogels for whole-culture encapsulation Perullini, Ana Mercedes Spedalieri, Ana Cecilia Biosensors Ecotoxicity Sol-gel Two-step procedure Whole-culture encapsulation Sol-gel encapsulation of living cells within inorganic hydrogels, mainly silica, is a promising technology for the design of biosensors. These host-guest functional materials maintain specific biologic functions of their guest while the properties of the host can be tuned to fulfill the requirements of particular applications. Inorganic immobilization hosts exhibit several advantages over their (bio)polymer-based counterparts. While both hosts provide tailored porosity, the former offers enhanced chemical stability towards biodegradation as well as higher physical stability (low swelling). However, inone-pot encapsulations, the direct contact of cells with precursors during the sol-gel synthesis and the constraints imposed by the inorganic matrix during operating conditions may influence the biological response. In order to prevent this, an alternative two-step procedure was proposed. Living cells are pre-encapsulated in biocompatible carriers based on biopolymers such as alginates that confer protection during the inorganic and more cytotoxic synthesis. By means of these carriers, whole cultures of microorganisms remain confined in small liquid volumes generated inside the inorganic host, providing near conventional liquid culture conditions. Moreover, this approach allows the encapsulation of multicellular organisms and the co-encapsulation of multiple isolated cultures within a single common monolithic host, creating an artificial ecosystem in a diminished scale isolated inside a nanoporous matrix that would allow ecotoxicity studies to be carried out in portable devices for on-line and in situ pollution level assessment. © 2015 by Nova Science Publishers, Inc. Fil:Perullini, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Spedalieri, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816346_v_n_p57_Perullini http://hdl.handle.net/20.500.12110/paper_97816346_v_n_p57_Perullini
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Biosensors
Ecotoxicity
Sol-gel
Two-step procedure
Whole-culture encapsulation
spellingShingle Biosensors
Ecotoxicity
Sol-gel
Two-step procedure
Whole-culture encapsulation
Perullini, Ana Mercedes
Spedalieri, Ana Cecilia
Inorganic hydrogels for whole-culture encapsulation
topic_facet Biosensors
Ecotoxicity
Sol-gel
Two-step procedure
Whole-culture encapsulation
description Sol-gel encapsulation of living cells within inorganic hydrogels, mainly silica, is a promising technology for the design of biosensors. These host-guest functional materials maintain specific biologic functions of their guest while the properties of the host can be tuned to fulfill the requirements of particular applications. Inorganic immobilization hosts exhibit several advantages over their (bio)polymer-based counterparts. While both hosts provide tailored porosity, the former offers enhanced chemical stability towards biodegradation as well as higher physical stability (low swelling). However, inone-pot encapsulations, the direct contact of cells with precursors during the sol-gel synthesis and the constraints imposed by the inorganic matrix during operating conditions may influence the biological response. In order to prevent this, an alternative two-step procedure was proposed. Living cells are pre-encapsulated in biocompatible carriers based on biopolymers such as alginates that confer protection during the inorganic and more cytotoxic synthesis. By means of these carriers, whole cultures of microorganisms remain confined in small liquid volumes generated inside the inorganic host, providing near conventional liquid culture conditions. Moreover, this approach allows the encapsulation of multicellular organisms and the co-encapsulation of multiple isolated cultures within a single common monolithic host, creating an artificial ecosystem in a diminished scale isolated inside a nanoporous matrix that would allow ecotoxicity studies to be carried out in portable devices for on-line and in situ pollution level assessment. © 2015 by Nova Science Publishers, Inc.
author Perullini, Ana Mercedes
Spedalieri, Ana Cecilia
author_facet Perullini, Ana Mercedes
Spedalieri, Ana Cecilia
author_sort Perullini, Ana Mercedes
title Inorganic hydrogels for whole-culture encapsulation
title_short Inorganic hydrogels for whole-culture encapsulation
title_full Inorganic hydrogels for whole-culture encapsulation
title_fullStr Inorganic hydrogels for whole-culture encapsulation
title_full_unstemmed Inorganic hydrogels for whole-culture encapsulation
title_sort inorganic hydrogels for whole-culture encapsulation
publishDate 2015
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816346_v_n_p57_Perullini
http://hdl.handle.net/20.500.12110/paper_97816346_v_n_p57_Perullini
work_keys_str_mv AT perullinianamercedes inorganichydrogelsforwholecultureencapsulation
AT spedalierianacecilia inorganichydrogelsforwholecultureencapsulation
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