Gaba metabolism in saccharomyces cerevisiae

The y-aminobutyric acid (GABA) is a non protein amino acid present in bacteria, plants, fungi and even in vertebrates. The role of GABA in the budding yeast Saccharomyces cerevisiae is not fully understood. GABA can be used by yeast as the sole nitrogen source and its carbon backbone enters the tric...

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Detalles Bibliográficos
Autores principales: Correa García, Susana Raquel, Bermudez Moretti, Mariana
Publicado: 2014
Materias:
GABA
Metabolism
Saccharomyces cerevisiae
Transcriptional regulation
Amino acids
Carboxylation
Nitrogen
Physiology
Aminobutyric acids
Direct oxidation
Nitrogen metabolism
Nitrogen sources
Subcellular compartments
Tricarboxylic acids
Yeast
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816332_v_n_p13_CorreaGarcia
http://hdl.handle.net/20.500.12110/paper_97816332_v_n_p13_CorreaGarcia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_97816332_v_n_p13_CorreaGarcia
record_format dspace
spelling paper:paper_97816332_v_n_p13_CorreaGarcia2023-06-08T16:38:30Z Gaba metabolism in saccharomyces cerevisiae Correa García, Susana Raquel Bermudez Moretti, Mariana GABA Metabolism Saccharomyces cerevisiae Transcriptional regulation Amino acids Carboxylation Metabolism Nitrogen Physiology Aminobutyric acids Direct oxidation GABA Nitrogen metabolism Nitrogen sources Subcellular compartments Transcriptional regulation Tricarboxylic acids Yeast The y-aminobutyric acid (GABA) is a non protein amino acid present in bacteria, plants, fungi and even in vertebrates. The role of GABA in the budding yeast Saccharomyces cerevisiae is not fully understood. GABA can be used by yeast as the sole nitrogen source and its carbon backbone enters the tricarboxylic acid (TCA) cycle. Three permeases are involved in extracellular GABA uptake: the general amino acid permease Gap1, the proline-specific permease Put4 and the GABA-specific permease Uga4. Once, within the cell, GABA is deaminated by the enzyme GABA transaminase, yielding glutamate and succinate semialdehyde. Glutamate enters the central nitrogen metabolism, while succinate semialdehyde is converted to succinate by the succinate semialhehyde dehydrogenase. Then, succinate enters the TCA cycle. However, most of the incorporated GABA is not catabolized but accumulated in a subcellular compartment, like the vacuole. On the other hand, GABA is synthesized in yeast cells by the decarboxylation of glutamate as an intermediate of the GABA shunt, by which a-ketoglutarate is converted to succinate. GABA shunt bypasses two steps of the TCA cycle and it is energetically less efficient than the direct oxidation of a-ketoglutarate at the TCA cycle. Strong evidence suggests that the GABA shunt plays a crucial role in protecting cells from stress. © 2014 by Nova Science Publishers, Inc. All rights reserved. Fil:Correa-García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bermúdez-Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816332_v_n_p13_CorreaGarcia http://hdl.handle.net/20.500.12110/paper_97816332_v_n_p13_CorreaGarcia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic GABA
Metabolism
Saccharomyces cerevisiae
Transcriptional regulation
Amino acids
Carboxylation
Metabolism
Nitrogen
Physiology
Aminobutyric acids
Direct oxidation
GABA
Nitrogen metabolism
Nitrogen sources
Subcellular compartments
Transcriptional regulation
Tricarboxylic acids
Yeast
spellingShingle GABA
Metabolism
Saccharomyces cerevisiae
Transcriptional regulation
Amino acids
Carboxylation
Metabolism
Nitrogen
Physiology
Aminobutyric acids
Direct oxidation
GABA
Nitrogen metabolism
Nitrogen sources
Subcellular compartments
Transcriptional regulation
Tricarboxylic acids
Yeast
Correa García, Susana Raquel
Bermudez Moretti, Mariana
Gaba metabolism in saccharomyces cerevisiae
topic_facet GABA
Metabolism
Saccharomyces cerevisiae
Transcriptional regulation
Amino acids
Carboxylation
Metabolism
Nitrogen
Physiology
Aminobutyric acids
Direct oxidation
GABA
Nitrogen metabolism
Nitrogen sources
Subcellular compartments
Transcriptional regulation
Tricarboxylic acids
Yeast
description The y-aminobutyric acid (GABA) is a non protein amino acid present in bacteria, plants, fungi and even in vertebrates. The role of GABA in the budding yeast Saccharomyces cerevisiae is not fully understood. GABA can be used by yeast as the sole nitrogen source and its carbon backbone enters the tricarboxylic acid (TCA) cycle. Three permeases are involved in extracellular GABA uptake: the general amino acid permease Gap1, the proline-specific permease Put4 and the GABA-specific permease Uga4. Once, within the cell, GABA is deaminated by the enzyme GABA transaminase, yielding glutamate and succinate semialdehyde. Glutamate enters the central nitrogen metabolism, while succinate semialdehyde is converted to succinate by the succinate semialhehyde dehydrogenase. Then, succinate enters the TCA cycle. However, most of the incorporated GABA is not catabolized but accumulated in a subcellular compartment, like the vacuole. On the other hand, GABA is synthesized in yeast cells by the decarboxylation of glutamate as an intermediate of the GABA shunt, by which a-ketoglutarate is converted to succinate. GABA shunt bypasses two steps of the TCA cycle and it is energetically less efficient than the direct oxidation of a-ketoglutarate at the TCA cycle. Strong evidence suggests that the GABA shunt plays a crucial role in protecting cells from stress. © 2014 by Nova Science Publishers, Inc. All rights reserved.
author Correa García, Susana Raquel
Bermudez Moretti, Mariana
author_facet Correa García, Susana Raquel
Bermudez Moretti, Mariana
author_sort Correa García, Susana Raquel
title Gaba metabolism in saccharomyces cerevisiae
title_short Gaba metabolism in saccharomyces cerevisiae
title_full Gaba metabolism in saccharomyces cerevisiae
title_fullStr Gaba metabolism in saccharomyces cerevisiae
title_full_unstemmed Gaba metabolism in saccharomyces cerevisiae
title_sort gaba metabolism in saccharomyces cerevisiae
publishDate 2014
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816332_v_n_p13_CorreaGarcia
http://hdl.handle.net/20.500.12110/paper_97816332_v_n_p13_CorreaGarcia
work_keys_str_mv AT correagarciasusanaraquel gabametabolisminsaccharomycescerevisiae
AT bermudezmorettimariana gabametabolisminsaccharomycescerevisiae
_version_ 1769175790799290368

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