Effect of flow in the formation of biofilms on different materials in drinking water distribution systems
Anywhere surface comes into contact with the water in a distribution system, biofilms are produced. Biofilms consist of microbial cells and their extracellular polymeric matrix (EPM) which facilitates bacterial attachment. The EPM consist of a complex mixture of cell derived exopolysaccharides (EPS)...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816183_v1_n_p620_Pujol http://hdl.handle.net/20.500.12110/paper_97816183_v1_n_p620_Pujol |
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paper:paper_97816183_v1_n_p620_Pujol2023-06-08T16:38:21Z Effect of flow in the formation of biofilms on different materials in drinking water distribution systems Biocorrosion Biofilm Biofilm formation capacity (BFC) Drinking water Flow condition Surface adherence Bacterial attachment Bacterial biofilm Bacterial colonization Bacterial count Biocorrosion Biofilm formation Biofilm structure Colorimetric methods Complex mixture Crystal violet Distribution systems Dynamic condition Exopolysaccharides Extracellular polymeric matrixes Flow condition Metallic material Metallic surface Microbial cells Staining method Static and dynamic Weight loss Biofilms Corrosion rate Iron Materials Nucleic acids Polypropylenes Potable water Proteins Thermoplastics Zinc Surfaces Anywhere surface comes into contact with the water in a distribution system, biofilms are produced. Biofilms consist of microbial cells and their extracellular polymeric matrix (EPM) which facilitates bacterial attachment. The EPM consist of a complex mixture of cell derived exopolysaccharides (EPS), proteins, lipids and nucleic acids. Biofilms can affect the corrosion rate of the surface. In this work, bacterial biofilms developed on different materials (commercial iron, zinc, copper and polypropylene) were submerged for 21 days in drinking water of La Plata city, were evaluated under flow and non-flow conditions. Three coupons of each material in both flow conditions were used for all determinations. Biofilm attached to the surface were removed. EPS and proteins were analyzed by standard colorimetric methods. Corrosion rates were estimated as the decrease in the weight of coupon. The relationship between biofilm and surface was determined by measuring biofilm formation capacity (BFC) using a crystal violet staining method under non-flow conditions. Commercial iron and zinc were the preferred materials for colonization, evaluated by bacterial counts, in both flow conditions. EPS and proteins were higher under dynamic condition, the highest values were found on commercial iron. All metallic materials exhibited weight loss at the end of the experiment, showing the same pattern of variation in the different materials under static and dynamic flow conditions. Commercial iron was the most affected material whilst the polypropylene did not show a weight loss. The highest BFC index was observed on commercial iron and zinc. These results show that the bacterial colonization was not affected by the flow condition but was sensitive to the kind of material. Biofilm structure was affected by both flow condition and the substrate. The presence of biofilm promotes metallic surface corrosion as evidenced by the existence of a relationship between weight loss and EPS and protein amounts, regardless of the flow condition. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816183_v1_n_p620_Pujol http://hdl.handle.net/20.500.12110/paper_97816183_v1_n_p620_Pujol |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biocorrosion Biofilm Biofilm formation capacity (BFC) Drinking water Flow condition Surface adherence Bacterial attachment Bacterial biofilm Bacterial colonization Bacterial count Biocorrosion Biofilm formation Biofilm structure Colorimetric methods Complex mixture Crystal violet Distribution systems Dynamic condition Exopolysaccharides Extracellular polymeric matrixes Flow condition Metallic material Metallic surface Microbial cells Staining method Static and dynamic Weight loss Biofilms Corrosion rate Iron Materials Nucleic acids Polypropylenes Potable water Proteins Thermoplastics Zinc Surfaces |
| spellingShingle |
Biocorrosion Biofilm Biofilm formation capacity (BFC) Drinking water Flow condition Surface adherence Bacterial attachment Bacterial biofilm Bacterial colonization Bacterial count Biocorrosion Biofilm formation Biofilm structure Colorimetric methods Complex mixture Crystal violet Distribution systems Dynamic condition Exopolysaccharides Extracellular polymeric matrixes Flow condition Metallic material Metallic surface Microbial cells Staining method Static and dynamic Weight loss Biofilms Corrosion rate Iron Materials Nucleic acids Polypropylenes Potable water Proteins Thermoplastics Zinc Surfaces Effect of flow in the formation of biofilms on different materials in drinking water distribution systems |
| topic_facet |
Biocorrosion Biofilm Biofilm formation capacity (BFC) Drinking water Flow condition Surface adherence Bacterial attachment Bacterial biofilm Bacterial colonization Bacterial count Biocorrosion Biofilm formation Biofilm structure Colorimetric methods Complex mixture Crystal violet Distribution systems Dynamic condition Exopolysaccharides Extracellular polymeric matrixes Flow condition Metallic material Metallic surface Microbial cells Staining method Static and dynamic Weight loss Biofilms Corrosion rate Iron Materials Nucleic acids Polypropylenes Potable water Proteins Thermoplastics Zinc Surfaces |
| description |
Anywhere surface comes into contact with the water in a distribution system, biofilms are produced. Biofilms consist of microbial cells and their extracellular polymeric matrix (EPM) which facilitates bacterial attachment. The EPM consist of a complex mixture of cell derived exopolysaccharides (EPS), proteins, lipids and nucleic acids. Biofilms can affect the corrosion rate of the surface. In this work, bacterial biofilms developed on different materials (commercial iron, zinc, copper and polypropylene) were submerged for 21 days in drinking water of La Plata city, were evaluated under flow and non-flow conditions. Three coupons of each material in both flow conditions were used for all determinations. Biofilm attached to the surface were removed. EPS and proteins were analyzed by standard colorimetric methods. Corrosion rates were estimated as the decrease in the weight of coupon. The relationship between biofilm and surface was determined by measuring biofilm formation capacity (BFC) using a crystal violet staining method under non-flow conditions. Commercial iron and zinc were the preferred materials for colonization, evaluated by bacterial counts, in both flow conditions. EPS and proteins were higher under dynamic condition, the highest values were found on commercial iron. All metallic materials exhibited weight loss at the end of the experiment, showing the same pattern of variation in the different materials under static and dynamic flow conditions. Commercial iron was the most affected material whilst the polypropylene did not show a weight loss. The highest BFC index was observed on commercial iron and zinc. These results show that the bacterial colonization was not affected by the flow condition but was sensitive to the kind of material. Biofilm structure was affected by both flow condition and the substrate. The presence of biofilm promotes metallic surface corrosion as evidenced by the existence of a relationship between weight loss and EPS and protein amounts, regardless of the flow condition. |
| title |
Effect of flow in the formation of biofilms on different materials in drinking water distribution systems |
| title_short |
Effect of flow in the formation of biofilms on different materials in drinking water distribution systems |
| title_full |
Effect of flow in the formation of biofilms on different materials in drinking water distribution systems |
| title_fullStr |
Effect of flow in the formation of biofilms on different materials in drinking water distribution systems |
| title_full_unstemmed |
Effect of flow in the formation of biofilms on different materials in drinking water distribution systems |
| title_sort |
effect of flow in the formation of biofilms on different materials in drinking water distribution systems |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816183_v1_n_p620_Pujol http://hdl.handle.net/20.500.12110/paper_97816183_v1_n_p620_Pujol |
| _version_ |
1769175835339653120 |