Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography

Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We...

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Detalles Bibliográficos
Publicado:	2018
Materias:	biologics downstream processing peptide ligands recombinant biopharmaceuticals solid-phase peptide synthesis transgenic cow milk Affinity chromatography Chelation Purification Synthesis (chemical) Biopharmaceuticals Cow milk Downstream-processing Peptide ligand Solid phase peptide synthesis Peptides
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v34_n4_p999_Saavedra http://hdl.handle.net/20.500.12110/paper_87567938_v34_n4_p999_Saavedra
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_87567938_v34_n4_p999_Saavedra
record_format	dspace
spelling	paper:paper_87567938_v34_n4_p999_Saavedra2023-06-08T16:36:56Z Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography biologics downstream processing peptide ligands recombinant biopharmaceuticals solid-phase peptide synthesis transgenic cow milk Affinity chromatography Chelation Purification Synthesis (chemical) biologics Biopharmaceuticals Cow milk Downstream-processing Peptide ligand Solid phase peptide synthesis Peptides Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked milk with the hormone as a sample, rhGH was purified with 88% yield and 92% purity in a single step with a fold purification of 4.5. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:999–1005, 2018. © 2018 American Institute of Chemical Engineers 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v34_n4_p999_Saavedra http://hdl.handle.net/20.500.12110/paper_87567938_v34_n4_p999_Saavedra
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	biologics downstream processing peptide ligands recombinant biopharmaceuticals solid-phase peptide synthesis transgenic cow milk Affinity chromatography Chelation Purification Synthesis (chemical) biologics Biopharmaceuticals Cow milk Downstream-processing Peptide ligand Solid phase peptide synthesis Peptides
spellingShingle	biologics downstream processing peptide ligands recombinant biopharmaceuticals solid-phase peptide synthesis transgenic cow milk Affinity chromatography Chelation Purification Synthesis (chemical) biologics Biopharmaceuticals Cow milk Downstream-processing Peptide ligand Solid phase peptide synthesis Peptides Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography
topic_facet	biologics downstream processing peptide ligands recombinant biopharmaceuticals solid-phase peptide synthesis transgenic cow milk Affinity chromatography Chelation Purification Synthesis (chemical) biologics Biopharmaceuticals Cow milk Downstream-processing Peptide ligand Solid phase peptide synthesis Peptides
description	Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked milk with the hormone as a sample, rhGH was purified with 88% yield and 92% purity in a single step with a fold purification of 4.5. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:999–1005, 2018. © 2018 American Institute of Chemical Engineers
title	Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography
title_short	Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography
title_full	Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography
title_fullStr	Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography
title_full_unstemmed	Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography
title_sort	single step recombinant human growth hormone (rhgh) purification from milk by peptide affinity chromatography
publishDate	2018
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v34_n4_p999_Saavedra http://hdl.handle.net/20.500.12110/paper_87567938_v34_n4_p999_Saavedra
_version_	1768541868870598656

Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography

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