Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey

Casein glycomacropeptide (CMP) is a 64- amino acid peptide found in cheese whey, which is released after κ-casein specific cleavage by chymosin. CMP lacks aromatic amino acids, a characteristic that makes it usable as a nutritional supplement for people with phenylketonuria. CMP consists of two nong...

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Autores principales:	Martínez, María Julia, Pilosof, Ana María Renata
Publicado:	2017
Materias:	bovine whey casein glycomacropeptide chitosan chromatography matrix wheat germ agglutinin Adsorption Affinity chromatography Amino acids Carboxylic acids Chitin Chitosan Chromatography Mammals Purification Adsorption capacities Aromatic amino acid Bovine whey Casein glycomacropeptide N-acetylneuraminic acid Nutritional supplements Purification process Wheat germ agglutinin Casein amino acid casein caseinomacropeptide n acetylneuraminic acid peptide fragment whey protein adsorption affinity chromatography animal bovine chemistry glycosylation isolation and purification metabolism milk whey Amino Acids Animals Caseins Cattle Chromatography, Affinity Glycosylation Milk N-Acetylneuraminic Acid Peptide Fragments Whey Whey Proteins
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v33_n1_p171_Baieli http://hdl.handle.net/20.500.12110/paper_87567938_v33_n1_p171_Baieli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_87567938_v33_n1_p171_Baieli2023-06-08T16:36:55Z Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey Martínez, María Julia Pilosof, Ana María Renata bovine whey casein glycomacropeptide chitosan chromatography matrix wheat germ agglutinin Adsorption Affinity chromatography Amino acids Carboxylic acids Chitin Chitosan Chromatography Mammals Purification Adsorption capacities Aromatic amino acid Bovine whey Casein glycomacropeptide N-acetylneuraminic acid Nutritional supplements Purification process Wheat germ agglutinin Casein amino acid casein caseinomacropeptide chitosan n acetylneuraminic acid peptide fragment whey protein adsorption affinity chromatography animal bovine chemistry glycosylation isolation and purification metabolism milk whey Adsorption Amino Acids Animals Caseins Cattle Chitosan Chromatography, Affinity Glycosylation Milk N-Acetylneuraminic Acid Peptide Fragments Whey Whey Proteins Casein glycomacropeptide (CMP) is a 64- amino acid peptide found in cheese whey, which is released after κ-casein specific cleavage by chymosin. CMP lacks aromatic amino acids, a characteristic that makes it usable as a nutritional supplement for people with phenylketonuria. CMP consists of two nonglycosylated isoforms (aCMP A and aCMP B) and its different glycosylated forms (gCMP A and gCMP B). The most predominant carbohydrate of gCMP is N-acetylneuraminic acid (sialic acid). Here, we developed a CMP purification process based on the affinity of sialic acid for wheat germ agglutinin (WGA). After formation of chitosan beads and adsorption of WGA, the agglutinin was covalently attached with glutaraldehyde. Two matrices with different WGA density were assayed for CMP adsorption. Maximum adsorption capacities were calculated according to the Langmuir model from adsorption isotherms developed at pH 7.0, being 137.0 mg/g for the matrix with the best performance. In CMP reduction from whey, maximum removal percentage was 79% (specifically 33.7% of gCMP A and B, 75.8% of aCMP A, and 93.9% of aCMP B). The CMP was recovered as an aggregate with an overall yield of 64%. Therefore, the matrices developed are promising for CMP purification from cheese whey. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:171–180, 2017. © 2016 American Institute of Chemical Engineers Fil:Martinez, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v33_n1_p171_Baieli http://hdl.handle.net/20.500.12110/paper_87567938_v33_n1_p171_Baieli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bovine whey casein glycomacropeptide chitosan chromatography matrix wheat germ agglutinin Adsorption Affinity chromatography Amino acids Carboxylic acids Chitin Chitosan Chromatography Mammals Purification Adsorption capacities Aromatic amino acid Bovine whey Casein glycomacropeptide N-acetylneuraminic acid Nutritional supplements Purification process Wheat germ agglutinin Casein amino acid casein caseinomacropeptide chitosan n acetylneuraminic acid peptide fragment whey protein adsorption affinity chromatography animal bovine chemistry glycosylation isolation and purification metabolism milk whey Adsorption Amino Acids Animals Caseins Cattle Chitosan Chromatography, Affinity Glycosylation Milk N-Acetylneuraminic Acid Peptide Fragments Whey Whey Proteins
spellingShingle	bovine whey casein glycomacropeptide chitosan chromatography matrix wheat germ agglutinin Adsorption Affinity chromatography Amino acids Carboxylic acids Chitin Chitosan Chromatography Mammals Purification Adsorption capacities Aromatic amino acid Bovine whey Casein glycomacropeptide N-acetylneuraminic acid Nutritional supplements Purification process Wheat germ agglutinin Casein amino acid casein caseinomacropeptide chitosan n acetylneuraminic acid peptide fragment whey protein adsorption affinity chromatography animal bovine chemistry glycosylation isolation and purification metabolism milk whey Adsorption Amino Acids Animals Caseins Cattle Chitosan Chromatography, Affinity Glycosylation Milk N-Acetylneuraminic Acid Peptide Fragments Whey Whey Proteins Martínez, María Julia Pilosof, Ana María Renata Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey
topic_facet	bovine whey casein glycomacropeptide chitosan chromatography matrix wheat germ agglutinin Adsorption Affinity chromatography Amino acids Carboxylic acids Chitin Chitosan Chromatography Mammals Purification Adsorption capacities Aromatic amino acid Bovine whey Casein glycomacropeptide N-acetylneuraminic acid Nutritional supplements Purification process Wheat germ agglutinin Casein amino acid casein caseinomacropeptide chitosan n acetylneuraminic acid peptide fragment whey protein adsorption affinity chromatography animal bovine chemistry glycosylation isolation and purification metabolism milk whey Adsorption Amino Acids Animals Caseins Cattle Chitosan Chromatography, Affinity Glycosylation Milk N-Acetylneuraminic Acid Peptide Fragments Whey Whey Proteins
description	Casein glycomacropeptide (CMP) is a 64- amino acid peptide found in cheese whey, which is released after κ-casein specific cleavage by chymosin. CMP lacks aromatic amino acids, a characteristic that makes it usable as a nutritional supplement for people with phenylketonuria. CMP consists of two nonglycosylated isoforms (aCMP A and aCMP B) and its different glycosylated forms (gCMP A and gCMP B). The most predominant carbohydrate of gCMP is N-acetylneuraminic acid (sialic acid). Here, we developed a CMP purification process based on the affinity of sialic acid for wheat germ agglutinin (WGA). After formation of chitosan beads and adsorption of WGA, the agglutinin was covalently attached with glutaraldehyde. Two matrices with different WGA density were assayed for CMP adsorption. Maximum adsorption capacities were calculated according to the Langmuir model from adsorption isotherms developed at pH 7.0, being 137.0 mg/g for the matrix with the best performance. In CMP reduction from whey, maximum removal percentage was 79% (specifically 33.7% of gCMP A and B, 75.8% of aCMP A, and 93.9% of aCMP B). The CMP was recovered as an aggregate with an overall yield of 64%. Therefore, the matrices developed are promising for CMP purification from cheese whey. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:171–180, 2017. © 2016 American Institute of Chemical Engineers
author	Martínez, María Julia Pilosof, Ana María Renata
author_facet	Martínez, María Julia Pilosof, Ana María Renata
author_sort	Martínez, María Julia
title	Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey
title_short	Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey
title_full	Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey
title_fullStr	Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey
title_full_unstemmed	Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey
title_sort	affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v33_n1_p171_Baieli http://hdl.handle.net/20.500.12110/paper_87567938_v33_n1_p171_Baieli
work_keys_str_mv	AT martinezmariajulia affinitychromatographymatricesfordepletionandpurificationofcaseinglycomacropeptidefrombovinewhey AT pilosofanamariarenata affinitychromatographymatricesfordepletionandpurificationofcaseinglycomacropeptidefrombovinewhey
_version_	1768545902471938048

Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey

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