Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures
ABSTRACT: Similar localizations of the Con A and wheat germ lectin receptors were obtained by using fluorescent lectins, in nonfixed spermatozoa and in spermatozoa fixed with formaldehyde and methanol, showing that in samples with the same previous treatment, worked out by the same operator, in whic...
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1985
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87558920_v9_n4_p124_NARDONE http://hdl.handle.net/20.500.12110/paper_87558920_v9_n4_p124_NARDONE |
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paper:paper_87558920_v9_n4_p124_NARDONE2023-06-08T16:36:52Z Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures Cerezo, Alberto Saúl cytochemical localization human spermatozoa oligomannosidic oligosaccharides lectin oligosaccharide cytochemistry cytology human male genital system mannose oligosaccharide oligosaccharide receptor priority journal spermatozoon Comparative Study Concanavalin A Fixatives Fluoresceins Formaldehyde Histocytochemistry Human Lectins Male Methanol Receptors, Concanavalin A Receptors, Mitogen Spermatozoa Support, Non-U.S. Gov't ABSTRACT: Similar localizations of the Con A and wheat germ lectin receptors were obtained by using fluorescent lectins, in nonfixed spermatozoa and in spermatozoa fixed with formaldehyde and methanol, showing that in samples with the same previous treatment, worked out by the same operator, in which enough determinations have been performed to eliminate individual variations, the different procedures of fixation produced similar results. The localizations obtained with fluorescent lectins confirm previous results, produced with the peroxidase technique, indicating that the lectins interact with oligomannosidic oligosaccharide receptors situated mainly in the equatorial segment of the acrosome and postnu‐clear cap. They also indicate the presence of similar receptors that were not detected previously on the neck and intermediate segment. The larger size of the lectin‐peroxidase‐diaminebenzidine reagent compared to that of the fluorescent lectins suggests that the new receptors are semicriptic and were not detected by steric effects in the first case, but were able to interact with lower volume, fluorescent probes. It is suggested that these oligomannosidic chains could be recognition signals for the elimination of incompetent sperm during their passage through the female reproductive track. Also these oligosaccharides and its possible metabolic variations could be involved in the interaction between the acrosome‐reacted spermatozoa with the zone pellucida. 1985 Munksgaard Fil:CEREZO, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87558920_v9_n4_p124_NARDONE http://hdl.handle.net/20.500.12110/paper_87558920_v9_n4_p124_NARDONE |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cytochemical localization human spermatozoa oligomannosidic oligosaccharides lectin oligosaccharide cytochemistry cytology human male genital system mannose oligosaccharide oligosaccharide receptor priority journal spermatozoon Comparative Study Concanavalin A Fixatives Fluoresceins Formaldehyde Histocytochemistry Human Lectins Male Methanol Receptors, Concanavalin A Receptors, Mitogen Spermatozoa Support, Non-U.S. Gov't |
| spellingShingle |
cytochemical localization human spermatozoa oligomannosidic oligosaccharides lectin oligosaccharide cytochemistry cytology human male genital system mannose oligosaccharide oligosaccharide receptor priority journal spermatozoon Comparative Study Concanavalin A Fixatives Fluoresceins Formaldehyde Histocytochemistry Human Lectins Male Methanol Receptors, Concanavalin A Receptors, Mitogen Spermatozoa Support, Non-U.S. Gov't Cerezo, Alberto Saúl Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures |
| topic_facet |
cytochemical localization human spermatozoa oligomannosidic oligosaccharides lectin oligosaccharide cytochemistry cytology human male genital system mannose oligosaccharide oligosaccharide receptor priority journal spermatozoon Comparative Study Concanavalin A Fixatives Fluoresceins Formaldehyde Histocytochemistry Human Lectins Male Methanol Receptors, Concanavalin A Receptors, Mitogen Spermatozoa Support, Non-U.S. Gov't |
| description |
ABSTRACT: Similar localizations of the Con A and wheat germ lectin receptors were obtained by using fluorescent lectins, in nonfixed spermatozoa and in spermatozoa fixed with formaldehyde and methanol, showing that in samples with the same previous treatment, worked out by the same operator, in which enough determinations have been performed to eliminate individual variations, the different procedures of fixation produced similar results. The localizations obtained with fluorescent lectins confirm previous results, produced with the peroxidase technique, indicating that the lectins interact with oligomannosidic oligosaccharide receptors situated mainly in the equatorial segment of the acrosome and postnu‐clear cap. They also indicate the presence of similar receptors that were not detected previously on the neck and intermediate segment. The larger size of the lectin‐peroxidase‐diaminebenzidine reagent compared to that of the fluorescent lectins suggests that the new receptors are semicriptic and were not detected by steric effects in the first case, but were able to interact with lower volume, fluorescent probes. It is suggested that these oligomannosidic chains could be recognition signals for the elimination of incompetent sperm during their passage through the female reproductive track. Also these oligosaccharides and its possible metabolic variations could be involved in the interaction between the acrosome‐reacted spermatozoa with the zone pellucida. 1985 Munksgaard |
| author |
Cerezo, Alberto Saúl |
| author_facet |
Cerezo, Alberto Saúl |
| author_sort |
Cerezo, Alberto Saúl |
| title |
Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures |
| title_short |
Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures |
| title_full |
Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures |
| title_fullStr |
Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures |
| title_full_unstemmed |
Cytochemical Characterization and Localization of Oligomannosidic Oligosaccharide Receptors on the Normal Human Spermatozoa Using Fluorescent Lectins: Comparison of Different Fixation Procedures |
| title_sort |
cytochemical characterization and localization of oligomannosidic oligosaccharide receptors on the normal human spermatozoa using fluorescent lectins: comparison of different fixation procedures |
| publishDate |
1985 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87558920_v9_n4_p124_NARDONE http://hdl.handle.net/20.500.12110/paper_87558920_v9_n4_p124_NARDONE |
| work_keys_str_mv |
AT cerezoalbertosaul cytochemicalcharacterizationandlocalizationofoligomannosidicoligosaccharidereceptorsonthenormalhumanspermatozoausingfluorescentlectinscomparisonofdifferentfixationprocedures |
| _version_ |
1768541583221719040 |