Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells

Since antiretroviral therapy suppresses but does not eradicate HIV-1 infection, methods to purge viral reservoirs are required. Many strategies involve the reactivation of chronically HIV infected cells to induce the expression of integrated viral genome. In this study, five bioactive compounds, the...

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Publicado:	2014
Materias:	1 cinnamoyl 3,11 dihydroxymeliacarpin 22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antigen p24 antiretrovirus agent curcumin interleukin 2 interleukin 6 interleukin 7 nordihydroguaiaretic acid plant medicinal product reactive oxygen metabolite stigmasterol tumor necrosis factor alpha unclassified drug 1-cinnamoyl-3,11-dihydroxymeliacarpin 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one biological factor cholestane derivative limonoid plant extract article cell viability controlled study cytokine production cytokine release drug mechanism human human cell Human immunodeficiency virus 1 infection lymphocyte monocyte macrophage precursor cell oxidation reduction state protein expression virus reactivation virus replication analogs and derivatives cell line DNA replication drug effects Human immunodeficiency virus 1 Human immunodeficiency virus infection metabolism monocyte tumor cell line U937 cell line virology Biological Factors Cell Line Cell Line, Tumor Cholestanones Curcumin DNA Replication HIV Infections HIV-1 Humans Interleukin-6 Limonins Masoprocol Monocytes Plant Extracts Reactive Oxygen Species Stigmasterol Tumor Necrosis Factor-alpha U937 Cells Virus Replication
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_23146133_v2014_n_p_Barquero http://hdl.handle.net/20.500.12110/paper_23146133_v2014_n_p_Barquero
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_23146133_v2014_n_p_Barquero
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spelling	paper:paper_23146133_v2014_n_p_Barquero2023-06-08T16:35:34Z Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells 1 cinnamoyl 3,11 dihydroxymeliacarpin 22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antigen p24 antiretrovirus agent curcumin interleukin 2 interleukin 6 interleukin 7 nordihydroguaiaretic acid plant medicinal product reactive oxygen metabolite stigmasterol tumor necrosis factor alpha unclassified drug 1-cinnamoyl-3,11-dihydroxymeliacarpin 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one biological factor cholestane derivative interleukin 6 limonoid plant extract reactive oxygen metabolite stigmasterol tumor necrosis factor alpha article cell viability controlled study cytokine production cytokine release drug mechanism human human cell Human immunodeficiency virus 1 infection lymphocyte monocyte macrophage precursor cell oxidation reduction state protein expression virus reactivation virus replication analogs and derivatives cell line DNA replication drug effects Human immunodeficiency virus 1 Human immunodeficiency virus infection metabolism monocyte tumor cell line U937 cell line virology virus replication Biological Factors Cell Line Cell Line, Tumor Cholestanones Curcumin DNA Replication HIV Infections HIV-1 Humans Interleukin-6 Limonins Masoprocol Monocytes Plant Extracts Reactive Oxygen Species Stigmasterol Tumor Necrosis Factor-alpha U937 Cells Virus Replication Since antiretroviral therapy suppresses but does not eradicate HIV-1 infection, methods to purge viral reservoirs are required. Many strategies involve the reactivation of chronically HIV infected cells to induce the expression of integrated viral genome. In this study, five bioactive compounds, the plant derivatives 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM), nordihydroguaiaretic acid (NDGA), and curcumin (Cur) and the synthetic stigmasterol analogs (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one (compound 2), were evaluated for their ability to elicit HIV replication in promonocytic (U1) and lymphocytic (H9+) HIV-1 chronically infected cells. The results revealed that natural compounds CDM, NDGA, and Cur were able to increase HIV-1 p24 antigen, determined by ELISA, only in latently infected promonocytic cells. CDM would reactivate HIV from latency by modulating the release of IL-6 and TNF-α, since the amount of both cytokines measured through ELISA significantly increased in U1 treated cells. Besides, NDGA increased ROS production, which might be related to the increase on p24 level observed in NDGA treated U1. These findings suggest that CDM, NDGA, and Cur might be candidates for further studies on latency-reversing therapeutics to eliminate latently HIV-1 reservoirs. © 2014 Andrea Alejandra Barquero et al. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_23146133_v2014_n_p_Barquero http://hdl.handle.net/20.500.12110/paper_23146133_v2014_n_p_Barquero
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	1 cinnamoyl 3,11 dihydroxymeliacarpin 22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antigen p24 antiretrovirus agent curcumin interleukin 2 interleukin 6 interleukin 7 nordihydroguaiaretic acid plant medicinal product reactive oxygen metabolite stigmasterol tumor necrosis factor alpha unclassified drug 1-cinnamoyl-3,11-dihydroxymeliacarpin 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one biological factor cholestane derivative interleukin 6 limonoid plant extract reactive oxygen metabolite stigmasterol tumor necrosis factor alpha article cell viability controlled study cytokine production cytokine release drug mechanism human human cell Human immunodeficiency virus 1 infection lymphocyte monocyte macrophage precursor cell oxidation reduction state protein expression virus reactivation virus replication analogs and derivatives cell line DNA replication drug effects Human immunodeficiency virus 1 Human immunodeficiency virus infection metabolism monocyte tumor cell line U937 cell line virology virus replication Biological Factors Cell Line Cell Line, Tumor Cholestanones Curcumin DNA Replication HIV Infections HIV-1 Humans Interleukin-6 Limonins Masoprocol Monocytes Plant Extracts Reactive Oxygen Species Stigmasterol Tumor Necrosis Factor-alpha U937 Cells Virus Replication
spellingShingle	1 cinnamoyl 3,11 dihydroxymeliacarpin 22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antigen p24 antiretrovirus agent curcumin interleukin 2 interleukin 6 interleukin 7 nordihydroguaiaretic acid plant medicinal product reactive oxygen metabolite stigmasterol tumor necrosis factor alpha unclassified drug 1-cinnamoyl-3,11-dihydroxymeliacarpin 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one biological factor cholestane derivative interleukin 6 limonoid plant extract reactive oxygen metabolite stigmasterol tumor necrosis factor alpha article cell viability controlled study cytokine production cytokine release drug mechanism human human cell Human immunodeficiency virus 1 infection lymphocyte monocyte macrophage precursor cell oxidation reduction state protein expression virus reactivation virus replication analogs and derivatives cell line DNA replication drug effects Human immunodeficiency virus 1 Human immunodeficiency virus infection metabolism monocyte tumor cell line U937 cell line virology virus replication Biological Factors Cell Line Cell Line, Tumor Cholestanones Curcumin DNA Replication HIV Infections HIV-1 Humans Interleukin-6 Limonins Masoprocol Monocytes Plant Extracts Reactive Oxygen Species Stigmasterol Tumor Necrosis Factor-alpha U937 Cells Virus Replication Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells
topic_facet	1 cinnamoyl 3,11 dihydroxymeliacarpin 22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antigen p24 antiretrovirus agent curcumin interleukin 2 interleukin 6 interleukin 7 nordihydroguaiaretic acid plant medicinal product reactive oxygen metabolite stigmasterol tumor necrosis factor alpha unclassified drug 1-cinnamoyl-3,11-dihydroxymeliacarpin 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one biological factor cholestane derivative interleukin 6 limonoid plant extract reactive oxygen metabolite stigmasterol tumor necrosis factor alpha article cell viability controlled study cytokine production cytokine release drug mechanism human human cell Human immunodeficiency virus 1 infection lymphocyte monocyte macrophage precursor cell oxidation reduction state protein expression virus reactivation virus replication analogs and derivatives cell line DNA replication drug effects Human immunodeficiency virus 1 Human immunodeficiency virus infection metabolism monocyte tumor cell line U937 cell line virology virus replication Biological Factors Cell Line Cell Line, Tumor Cholestanones Curcumin DNA Replication HIV Infections HIV-1 Humans Interleukin-6 Limonins Masoprocol Monocytes Plant Extracts Reactive Oxygen Species Stigmasterol Tumor Necrosis Factor-alpha U937 Cells Virus Replication
description	Since antiretroviral therapy suppresses but does not eradicate HIV-1 infection, methods to purge viral reservoirs are required. Many strategies involve the reactivation of chronically HIV infected cells to induce the expression of integrated viral genome. In this study, five bioactive compounds, the plant derivatives 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM), nordihydroguaiaretic acid (NDGA), and curcumin (Cur) and the synthetic stigmasterol analogs (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one (compound 2), were evaluated for their ability to elicit HIV replication in promonocytic (U1) and lymphocytic (H9+) HIV-1 chronically infected cells. The results revealed that natural compounds CDM, NDGA, and Cur were able to increase HIV-1 p24 antigen, determined by ELISA, only in latently infected promonocytic cells. CDM would reactivate HIV from latency by modulating the release of IL-6 and TNF-α, since the amount of both cytokines measured through ELISA significantly increased in U1 treated cells. Besides, NDGA increased ROS production, which might be related to the increase on p24 level observed in NDGA treated U1. These findings suggest that CDM, NDGA, and Cur might be candidates for further studies on latency-reversing therapeutics to eliminate latently HIV-1 reservoirs. © 2014 Andrea Alejandra Barquero et al.
title	Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells
title_short	Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells
title_full	Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells
title_fullStr	Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells
title_full_unstemmed	Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells
title_sort	naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_23146133_v2014_n_p_Barquero http://hdl.handle.net/20.500.12110/paper_23146133_v2014_n_p_Barquero
_version_	1768544106674388992

Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells

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